Recombinant Mouse Cation-dependent mannose-6-phosphate receptor (M6pr)

Code CSB-CF013293MO
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
M6pr
Uniprot No.
Alternative Names
M6pr; 46mpr; Cation-dependent mannose-6-phosphate receptor; CD Man-6-P receptor; CD-MPR; 46 kDa mannose 6-phosphate receptor; MPR 46
Species
Mus musculus (Mouse)
Expression Region
22-278
Target Protein Sequence
VRESWQIEEKSCDLVGEKDKESKNEVALLERLRPLFNKSFESTVGQGSDTYSYIFRVCREASNHSSGAGLVQINKSNDKETVVGRINETHIFNGSNWIMLIYKGGDEYDNHCGKEQRRAVVMISCNRHTLAANFNPVSEERGKVQDCFYLFEMDSSLACSPEVSHLSVGSILLVIFASLVAVYIIGGFLYQRLVVGAKGMEQFPHLAFWQDLGNLVADGCDFVCRSKPRNVPAAYRGVGDDQLGEESEERDDHLLPM
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
Gene References into Functions
  1. Finally, Chlamydia trachomatis infection interferes with the SNX5:cation-independent mannose-6-phosphate receptor interaction, suggesting that Chlamydia IncE and cation-independent mannose-6-phosphate receptor are dependent on the same binding surface on SNX5. PMID: 28252385
  2. We conclude that M6PR is an important receptor used by HIV-1 to cross the blood-brain barrier. PMID: 22761827
  3. Mannose receptor interacts with Fc receptors and is critical for the development of crescentic glomerulonephritis in mice PMID: 20407205
  4. Mannose 6-phosphate receptor has a role in acid sphingomyelinase deficiency PMID: 14557264
  5. in hepatocytes of MPR-deficient neonatal mice lysosomal storage occurs when both MPRs are lacking, whereas deficiency of CI-MPR only has no effect on the ultrastructure of the lysosomal system. PMID: 14585283
  6. Data characterize the retrograde transport of mannose 6-phosphate receptor MPR46 from endosomes to the trans-Golgi network by an in vitro transport assay using mouse fibroblast cell lines. PMID: 14617361
  7. there is not a an Mpr300- or Mpr46-dependent pathway of granzyme-mediated killing in internalization of monomeric and complexed granzyme B PMID: 14985351
  8. These results indicate that beta-glucuronidase transport into brain parenchyma in early postnatal life is mediated by the mannose 6-phosphate/insulin-like growth factor II receptor. PMID: 15314220
  9. Mpr46 is dispensable on target cells for lysis and apoptosis mediated by alloreactive cytotoxic T lymphocytes. PMID: 15371683
  10. The murine 46-kDa mannose 6-phosphate receptor has the capacity to deliver its cargo proteins to lysosomes even in the absence of endosomal acidification. PMID: 17105763
  11. epinephrine restores the M6P receptor-mediated functional transport of (131)I-P-beta-glucuronidase across the blood brain barrier in adults to levels seen in the neonate PMID: 17646643
  12. the alpha-galactosidase enzyme is incorporated via cation-independent mannose 6-phosphate receptors in Schwann cells PMID: 17965825

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Subcellular Location
Lysosome membrane; Single-pass type I membrane protein.
Database Links
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