Recombinant Mouse Elongation of very long chain fatty acids protein 6 (Elovl6)

Code CSB-CF856352MO
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Elovl6
Uniprot No.
Alternative Names
Elovl6; Face; Lce; Masr; Elongation of very long chain fatty acids protein 6; 3-keto acyl-CoA synthase Elovl6; ELOVL fatty acid elongase 6; ELOVL FA elongase 6; Fatty acyl-CoA elongase; Long chain fatty acid elongase; Myelin-associated SUR4 protein; Very long chain 3-ketoacyl-CoA synthase 6; Very long chain 3-oxoacyl-CoA synthase 6
Species
Mus musculus (Mouse)
Expression Region
1-267
Target Protein Sequence
MNMSVLTLQEYEFEKQFNENEAIQWMQENWKKSFLFSALYAAFIFGGRHLMNKRAKFELR KPLVLWSLTLAVFSIFGALRTGAYMLYILMTKGLKQSVCDQSFYNGPVSKFWAYAFVLSK APELGDTIFIILRKQKLIFLHWYHHITVLLYSWYSYKDMVAGGGWFMTMNYGVHAVMYSY YALRAAGFRVSRKFAMFITLSQITQMLMGCVINYLVFNWMQHDNDQCYSHFQNIFWSSLM YLSYLVLFCHFFFEAYIGKVKKATKAE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that elongates fatty acids with 12, 14 and 16 carbons with higher activity toward C16:0 acyl-CoAs. Catalyzes the synthesis of unsaturated C16 long chain fatty acids and, to a lesser extent, C18:0 and those with low desaturation degree. May participate in the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
Gene References into Functions
  1. Elovl6 is a fundamental factor linking dysregulated lipid metabolism to beta-cell dysfunction, islet inflammation, and beta-cell apoptosis in T2D, highlighting oleate as the potential culprit of beta-cell lipotoxicity. PMID: 28461456
  2. data suggest that Elovl6 plays a critical role for proper development of embryonic growth plate PMID: 27467521
  3. the synergistic action of ChREBP and SREBP-1c is necessary for the maximal induction of Elovl6 expression in the liver. PMID: 27524233
  4. These findings suggest that Elovl6-mediated changes in hepatic FA composition, especially oleic acid (C18:1n-9), control handling of hepatic cholesterol and BA, which protects against hepatotoxicity and steatohepatitis PMID: 26619823
  5. Elongation of Very Long Chain fatty acids 6 (Elovl6) is necessary for the thermogenic action of brown adipose tissue PMID: 26628376
  6. Data (including data from studies in knockout mice) confirm Elovl6 as only enzyme elongating palmitate to oleate; elongation of palmitoleate to vaccinate does not require Elovl6; deletion of Elovl6 does not prevent nonalcoholic fatty liver disease. PMID: 25281760
  7. Our findings suggest that Elovl6 could be involved in insulin secretory capacity per beta-cell and diabetes. PMID: 24938128
  8. Elovl6 deficiency results in a change in fatty acid composition, induction of apoptosis, TGF-beta1 expression and reactive oxygen species generation. PMID: 24113622
  9. Elovl6 is a critical modulator for atherogenic high-fat diet-induced inflammation, oxidative stress, and fibrosis in the liver. PMID: 22753171
  10. REVIEW: fatty acid metabolism and lipotoxicity; discussion of the role of Elovl6 in newly recognized aspects of metabolic regulation PMID: 22266797
  11. Elovl6 in macrophages may contribute to foam cell formation and progression of atherosclerosis. PMID: 21817094
  12. data indicate this elongase is a lipogenic enzyme regulated by SREBP-1, playing an important role in de novo synthesis of long-chain saturated and monosaturated fatty acids in conjunction with fatty acid synthase and stearoyl-CoA desaturase. PMID: 12032166
  13. These data demonstrated that Elovl-6 is regulated directly and primarily by SREBP-1c. PMID: 18226595
  14. Change in composition of fatty acids due to Elovl-6 is the dicisive factor for gene expression of energy metabolism and life-style associated disorders. (review) PMID: 18810844
  15. Studies suggest that inhibition of ELOVL6 could be a new therapeutic approach for the treatment of insulin resistance, diabetes, cardiovascular disease, and other metabolic diseases. PMID: 19259639

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
ELO family, ELOVL6 subfamily
Tissue Specificity
Highly expressed in adrenal gland, liver, white adipose tissue (WAT), adult and fetal brain, cerebellum, spinal cord, testis, skin and peripheral nerve; where lipogenesis and steroidogenesis are active. Weakly expressed in kidney, heart, skeletal muscle,
Database Links
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