Recombinant Mouse Insulin receptor(Insr)

Code CSB-CF011753MO
Size Pls inquire other sizes
Source in vitro E.coli expression system
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Product Details

Target Names Insr
Uniprot No. P15208
Alternative Names Insr; Insulin receptor; IR; EC 2.7.10.1; CD antigen CD220) [Cleaved into: Insulin receptor subunit alpha; Insulin receptor subunit beta]
Species Mus musculus (Mouse)
Expression Region 28-748
Target Protein Sequence HLYPGEVCPGMDIRNNLTRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDLFPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSVEDNYIVLNKDDNEECGDVCPGTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHKECLGNCSEPDDPTKCVACRNFYLDGQCVETCPPPYYHFQDWRCVNFSFCQDLHFKCRNSRKPGCHQYVIHNNKCIPECPSGYTMNSSNLMCTPCLGPCPKVCQILEGEKTIDSVTSAQELRGCTVINGSLIINIRGGNNLAAELEANLGLIEEISGFLKIRRSYALVSLSFFRKLHLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLTITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELLKFSFIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPQRSNDPKSQTPSHPGWLMRGLKPWTQYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVYWERQAEDSELFELDYCLKGLKLPSRTWSPPFESDDSQKHNQSEYDDSASECCSCPKTDSQILKELEESSFRKTFEDYLHNVVFVPRPSRKRRSLEEVGNVTATTLTLPDFPNVSS
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways
Gene References into Functions
  1. Loss of Endothelial IR Impairs Barrier Function in the Brain. PMID: 28923931
  2. in beta cells, INSR-B has a protective role, while INSR-A expression sensitizes beta cells to programmed cell death. PMID: 27526875
  3. we show that glucagon receptor (GCGR) inhibition with a monoclonal antibody normalized blood glucose and beta-hydroxybutyrate levels. Insulin receptor antagonism increased pancreatic beta-cell mass threefold. Normalization of blood glucose levels with GCGR-blocking antibody unexpectedly doubled beta-cell mass relative to that observed with S961 alone and 5.8-fold over control PMID: 28115707
  4. Data (including data from studies in knockout mice) suggest double knockout (DKO) mice lacking Insr and Igf1r exhibit obesity with insulin resistance and increased adiposity; on high-fat diet, DKO mice exhibit metabolic syndrome. (Insr = insulin receptor; Igf1r = insulin-like growth factor I receptor) PMID: 29040448
  5. The data in this paper demonstrate that IR knockdown in primary tumors partially reverses the growth-promoting effects of hyperinsulinemia as well as highlighting the importance of the insulin receptor signaling pathway in cancer progression, and more specifically in epithelial-mesenchymal transition. PMID: 27435064
  6. long-term hepatic expression of IRA could be a promising therapeutic approach for the treatment of type 2 diabetes mellitus. PMID: 27562101
  7. the overlap of IR and IGF1R signaling is critical to the regulation of muscle protein turnover, and this regulation depends on suppression of FoxO-regulated, autophagy-mediated protein degradation PMID: 27525440
  8. These data reveal a critical pathophysiological role for INSR Thr1160 phosphorylation and provide further mechanistic links between PKCepsilon and INSR in mediating Nonalcoholic fatty liver disease -induced hepatic insulin resistance. PMID: 27760050
  9. Insr was downregulated in the arcuate nucleus of type 2 diabetic mice. PMID: 28456145
  10. Mice lacking the insulin receptor in AgRP neurons (AgRP IR KO) exhibited impaired hepatic insulin action because the ability of insulin to suppress hepatic glucose production (hGP) was reduced, but the ability of insulin to suppress lipolysis was unaltered. To the contrary, in POMC IR KO mice, insulin lowered hGP but failed to suppress adipose tissue lipolysis. PMID: 28385803
  11. Intracellular retention of the insulin receptor is caused by elevated amounts of alpha-taxilin, a free syntaxin binding protein, in HBV expressing hepatocytes preventing proper targeting of the insulin receptor to the cell surface. PMID: 27155659
  12. Results found that glioblastoma tumors resistant to PDGFR inhibition required the expression and activation of the insulin receptor (IR)/insulin growth-like factor receptor (IGF1R) for tumor cell proliferation and survival. PMID: 28138037
  13. IR is critical in adipocyte maintenance. PMID: 28065828
  14. The IR in the intestinal epithelium plays important roles in intestinal gene expression, glucose uptake, and GIP production, which may contribute to pathophysiological changes in individuals with diabetes, metabolic syndrome, and other insulin-resistant states. PMID: 28096258
  15. In conclusion, we have identified that ARL15 acts as an insulin-sensitizing effector molecule to upregulate the phosphorylation of members of the canonical IR/IRS1/PDPK1/AKT insulin pathway by interacting with its GAP ASAP2 and activating PDPK1. This research may provide new insights into GTPase-mediated insulin signalling regulation and facilitate the development of new pharmacotherapeutic targets for insulin sensitizati PMID: 28322786
  16. Data suggest IGT10 mice, diabetes type 2 model, exhibit 2 genetic defects: haploinsufficiency (heterozygosity for null allele) of insulin receptor (Insr); splice-site mutation in protein phosphatase 2 regulatory subunit B alpha (Ppp2r2a). Inheritance of either allele results in insulin resistance but not overt diabetes. Double heterozygosity leads to insulin resistance and diabetes type 2 without increase in body weight. PMID: 26868295
  17. adipocyte IR function is crucial to systemic energy metabolism and has profound effects on adiposity, hepatic homeostasis and lifespan PMID: 27246738
  18. ZIP14-mediated zinc transport contributes to regulation of endosomal insulin receptor activity and glucose homeostasis in hepatocytes. PMID: 27703010
  19. Suggest that overexpression of IGF-IR or IRA isoform, as homodimers or as part of IRA/IGF-IR hybrid receptors, confers a stronger migratory capability to vascular smooth muscle cells and might occur in early stages of atherosclerotic process. PMID: 27905925
  20. Acute knockdown of Insr or both Irs1 and Irs2 in adipocytes increased Adipoq mRNA expression but reduced adiponectin secretion. PMID: 26888756
  21. unsuppressed lipolysis in adipocytes elicited by HFD feeding is linked with enhanced gluconeogenesis from glycerol and with alterations in BA physiology in Insr(P1195L/+)/HFD liver. PMID: 26615883
  22. NF-kappaB-miR-195/497-Igf1r/Insr-Ccnd2/Ccne1 plays important roles in myogenesis. PMID: 26567220
  23. Therefore, overexpression of insulin receptor improves obese and diabetic phenotypes in db/db mice, with consequences on growth. PMID: 26096452
  24. only Ptprj was co-expressed with the IR in major insulin target tissues : the skeletal muscle, liver and adipose tissue. the activation of IR and Akt by insulin was enhanced, and glucose and insulin tolerance was improved in Ptprj-deficient mice PMID: 26063811
  25. Data reports that IRA in beta cells confers a stronger proliferation capability favoring the mitogenic effects of IGF-I and also increasing glucose uptake of these cells, a key factor in pancreatic beta cell proliferation. PMID: 25797178
  26. Our results indicate that the insulin receptor may have some role in controlling the rate of rod response decay, but they exclude a major role of the insulin receptor pathway in phototransduction. PMID: 25598343
  27. Mutation in the insulin receptor attenuated the oxidative stress and apoptosis in beta-cells. PMID: 25295420
  28. Our results highlight the mitogenic role of the IR in mammary tumor progression with a direct link to CD24 expression PMID: 25694511
  29. Deletion of the insulin receptor alone or in combination with the IGF-1 receptor or treatment with rapamycin prevented hyperphosphorylation of S6RP without affecting the mitochondrial structural defect in PHB2-deficient animals. PMID: 25643582
  30. Data suggest that insulin/insulin receptor-stimulated GLUT4 (facilitated glucose transporter member 4) translocation to plasma membrane in adipocytes may require assembly of SNARE protein complexes. [REVIEW] PMID: 25233421
  31. Data indicate that insulin and insulin-like growth factor 1 receptors (IR and IGF1R) signals that regulating expression of imprinted genes and miRNAs through transcriptional mechanisms distinct from classical imprinting control. PMID: 25246545
  32. Leptin knockouts are hyperphagic and obese, whereas insulin receptor knockouts are similar to controls but double knockouts exihibit higher body weight and adiposity solely due to reduced energy expenditure. PMID: 25125486
  33. insulin receptor expression in osteoblasts is critically important for proper bone development and maintenance of structural integrity PMID: 24963495
  34. Male insulin receptor knock-out mice exhibited significantly augmented LH concentration and a trend toward reduced seminal vesicle weight compared with control mice, which may be indicative of primary hypogonadism. PMID: 25116708
  35. MsrA and protein oxidation play a role in the regulation of glucose homeostasis; data support a novel hypothesis that obesity-induced insulin resistance is caused in part by reduced function of insulin signaling proteins arising from protein oxidation PMID: 23089224
  36. Nox2-derived ROS played a key role in damaging insulin receptor and endothelial function in dietary obesity after middle-age. PMID: 23957783
  37. GM3 supplementation or inhibition of IGF-1R or PI3K reverses the increased migration of GM3S(-/-) keratinocytes, whereas IR knockdown only partially suppresses migration. PMID: 24326453
  38. GH signaling in mouse calvarial cells depends on insulin-like growth factor-1 receptor (IGF-1R), but not insulin receptor (IR) PMID: 24302626
  39. IGF-II promotes stemness of neural stem cells via the IR-A and not through activation of either the IGF-1R or the IGF-2R. PMID: 24398690
  40. SIRP-alpha is part of a novel mechanism for inflammation-mediated insulin resistance in muscle and muscle wasting in chronic kidney disease. PMID: 23515050
  41. LMBD1 plays an imperative role in mediating and regulating the endocytosis of the IR. PMID: 24078630
  42. The insulin/IGF signaling pathway is required for FSH-mediated Sertoli cell proliferation. PMID: 23518924
  43. We conclude that insulin interacting with IR is essential for mammary differentiation during murine pregnancy PMID: 23982156
  44. these results support a role for insulin receptor in the proximal tubule in the modulation of systemic glucose levels. PMID: 23723425
  45. Data indicate that insulin receptor (InsR) is important for epithelial sodium channel (ENaC) activity. PMID: 23558339
  46. Insulin receptor signaling in cones. PMID: 23673657
  47. These results suggest that insulin stimulates the association of insulin receptor with syndecan-1 and the complex formation of syndecan-1 and integrin could play an important role in ERK I/II-ALP signaling pathway in osteoblast cells. PMID: 23252577
  48. Data from knockout mice and cocultured hepatocytes/sinusoidal endothelial cells suggest that up-regulation (not down-regulation) of insulin/Insr/insulin receptor substrate 1 signaling in liver sinusoidal endothelium leads mice to insulin resistance. PMID: 23349480
  49. These findings indicate that prior to sex determination somatic progenitors in Insr;Igf1r mutant gonads are not lineage primed and thus incapable of upregulating/repressing the male and female genetic programs required for cell fate restriction. PMID: 23300479
  50. Data indicate that insulin receptors in pro-opiomelanocortin neurons (IR/LepR(POMC) mice may serve as a new mouse model to clarify the involvement of adipose and liver tissue in the pathogenesis and etiology of polycystic ovary syndrome (PCOS). PMID: 23119079
  51. Synaptic plasticity and recognition memory are impaired in IR beta-subunit heterozygous mice. PMID: 22661254
  52. There is a fundamental role for insulin through its classic receptor in the modulation of electrolyte homeostasis and blood pressure. PMID: 23195676
  53. Data indicate that knockdown of Crebh or Cb1r antagonism attenuated 2-arachidonoylglycerol mediated induction of Lipin1 gene expression and decreased diacylglycerol production in liver and subsequently restored insulin receptor signaling. PMID: 22989885
  54. cell-autonomous role of InsR/FoxO1 signaling in regulating POMC neuron number, distinct from its established role to activate Pomc transcription. PMID: 22319636
  55. expression of adipocytokines is associated with brown fat lipoatrophy and increases visceral adiposity on triggering vascular insulin resistance and dysfunction in brown adipose tissue (BAT) insulin receptor knockout (BATIRKO) mice PMID: 22253415
  56. These data provide evidence that human beta- and alpha-cells can enter the cell-cycle, but proliferation of beta-cells in type 2 diabetes mellitus fails due to G1-to-S phase arrest secondary to defective insulin signaling. PMID: 22140505
  57. Defective insulin signaling is involved in accelerated atherosclerosis in Insr(+/-)Irs1(+/-)Apoe(-/-) mice by promoting vascular dysfunction and inflammation. PMID: 22199371
  58. Identification of a molecular activator for insulin receptor with potent anti-diabetic effects. PMID: 21908618
  59. Deletion of interleukin-6 improves pyruvate tolerance without altering hepatic insulin signaling in the leptin receptor-deficient mouse. PMID: 21632071
  60. Loss of placental Cited1 leads to intrauterine organ growth restriction with decreased IGF-I and IGF-II expression and in IR and Igf1r signaling. PMID: 21486933
  61. The methylation levels are increased in the Igf1 receptor (but not the insulin receptor) promoter in skeletal muscle of db/db male mice. PMID: 21474992
  62. a novel AD mouse model carrying the same insulin receptor mutation showed that the combination of insulin resistance and hyperinsulinemia did not accelerate plaque formation or memory abnormalities in these mice. PMID: 21549686
  63. The findings provide direct evidence for a functional interaction between CB1R and IR signaling involved in the regulation of beta-cell proliferation. PMID: 21346174
  64. Data indicate that IR and IGF1R have bidirectional roles in the control of cell survival and can be viewed as dependence receptors. PMID: 21139139
  65. Data suggest that insulin receptor signaling in the pituitary is required for the reproductive impairment seen in diet-induced obesity and that gonadotrophs maintain insulin sensitivity in a setting of peripheral insulin resistance. PMID: 20816095
  66. insulin receptor and key targets of the insulin signaling pathway are activated differently from insulin by oligomers of grape-seed procyanidin extract PMID: 19443198
  67. Study shows that insulin receptor (IR) signaling in osteoblasts controls osteoblast development and osteocalcin expression by suppressing the Runx2 inhibitor Twist2. PMID: 20655471
  68. IR and IGF1R act as identical portals to the regulation of gene expression, with differences between insulin and IGF-1 effects due to a modulation of the amplitude of the signal created by the specific ligand-receptor interaction PMID: 20360006
  69. retinal insulin receptor-activated neuroprotective survival signal in mice lacking the protein-tyrosine phosphatase-1B gene PMID: 20061388
  70. Data showed that the female reproductive functions are not affected when Insr, Igf1r or both Insr;Igf1r are ablated in oocytes. PMID: 19851056
  71. Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor PMID: 11782469
  72. To investigate the role of insulin signaling on postnatal cardiac development, physiology, and cardiac metabolism, we generated mice with a cardiomyocyte-selective insulin receptor knockout (CIRKO) using cre/loxP recombination. PMID: 11877471
  73. Peptides identify the critical hotspots involved in the biological activation of the insulin receptor PMID: 11964401
  74. role in catalysing tyrosine phosphorylation of caveolin-1 PMID: 12036959
  75. Insulin-IGF1 hybrid receptors have different tissue-specific responses based on their isoforms PMID: 12138094
  76. In mice deficient in this protein, transgenic overexpression of glucokinase in the liver improves metabolic disorders PMID: 12242462
  77. role of activation in secretion of annexin II PMID: 12431980
  78. The IR may serve as a cellular substrate for both protein tyrosine phosphatase forms TC48 and TC45. Differentially localized variants of TCPTP may dephosphorylate the IR and downregulate insulin-induced signaling in vivo. PMID: 12612081
  79. Mice heterozygous for deletion of this gene are likely to develop diabetes. PMID: 12765966
  80. IR in muscle and adipose tissue is critical for regulation of adiposity [review] PMID: 12788932
  81. role in retinal neovascularization through the expression of vascular mediators PMID: 12813019
  82. the insulin receptor tyrosine kinase family, comprising Ir, Igf1r and Irr, is required for the appearance of male gonads and thus for male sexual differentiation PMID: 14628051
  83. The insulin receptor knockout mouse confirms importance of white adipose tissue plasticity in maintenance of whole body insulin sensitivity and is an interesting model to search for new secreted molecules that positively alter adipose tissue biology. PMID: 14684612
  84. the number of insulin receptors is an important determinant of the specificity of insulin action PMID: 14722613
  85. These observations indicate that neither insulin nor IGF-1 signaling in vascular endothelial cells is required for development and maintenance of BBB or BRB. PMID: 15063759
  86. Intrinsic heterogeneity in adipose tissue of fat-specific insulin receptor knock-out mice is associated with differences in patterns of gene expression. PMID: 15131119
  87. Data show that restoration of insulin receptor function in brain, liver, and pancreatic beta cells rescues insulin receptor knockout mice from neonatal death, prevents diabetes, and normalizes adipose tissue content, lifespan, and reproductive function. PMID: 15254588
  88. Knockout mice had increased systolic blood pressure and increase in tension of phenylephrine preconstricted aortic rings in response to NO synthase inhibitors PMID: 15448096
  89. the insulin receptor has roles in islet beta cell gene expression and function PMID: 15546857
  90. skeletal muscle gene-expression profiles of muscle insulin receptor knockout (MIRKO) mice and their Lox controls in the basal, streptozotocin-induced diabetic, and insulin-treated diabetic states PMID: 15546994
  91. binding of IGF2 to insulin receptor isoform A induces a partially different gene expression profile from insulin binding PMID: 15650270
  92. Homozygous mutants died in the neonatal stage whereas heterozygotes showed the suppressed kinase activity of the insulin receptor but without developing diabetes PMID: 15713666
  93. In response to hypoglycemia, epinephrine levels rose 5.7-fold in controls but only 3.5-fold in insulin receptor knockout mice. PMID: 15855332
  94. restoration of hepatic insulin signaling in knockout mice fails to normalize the in vivo response to insulin PMID: 15864351
  95. Insulin receptor substrate proteins regulate a necdin-E2F4 interaction that represses peroxisome-proliferator-activated receptor gamma (PPARgamma) transcription via a cyclic AMP response element binding protein (CREB)-dependent pathway. PMID: 15895078
  96. Timp3 has a role in diabetes and vascular inflammation through the insulin receptor and TNF-alpha PMID: 16294222
  97. Western blot analysis revealed reduced Akt expression and insulin (5 mU g(-1))-stimulated Akt phosphorylation, elevated PTP1B expression and diminished basal insulin receptor tyrosine phosphorylation. PMID: 16626396
  98. IR mediates glucose uptake through its specific association with endogenous, but not with exogenous, glucose transporters in neonatal hepatocytes PMID: 16644916
  99. Reduced insulin receptor signaling in bone is not a major factor contributing to bone loss in type I diabetes. PMID: 16973725
  100. IR protein expression is regionally different in males and females, gender-dependent, and modulated during the estrus cycle. It isn't modified on inhibition of oxidative phosphorylation in any region in females and altered in hippocampus solely in males. PMID: 17086487

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Database Links

KEGG: mmu:16337

STRING: 10090.ENSMUSP00000088837

UniGene: Mm.268003

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