Recombinant Mouse Interferon-induced transmembrane protein 5 (Ifitm5)

Code CSB-CF011028MO
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Ifitm5
Uniprot No.
Alternative Names
Ifitm5; Bril; Fragilis4; Interferon-induced transmembrane protein 5; Bone-restricted interferon-induced transmembrane protein-like protein; Dispanin subfamily A member 1; DSPA1; Fragilis family member 4
Species
Mus musculus (Mouse)
Expression Region
1-134
Target Protein Sequence
MDTSYPREDPRAPSSRKADAAAHTALSMGTPGPTPRDHMLWSVFSTMYLNLCCLGFLALV HSVKARDQKMAGNLEAARQYGSKAKCYNILAAMWTLVPPLLLLGLVVTGALHLSKLAKDS AAFFSTKFDEEDYN
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for normal bone mineralization.
Gene References into Functions
  1. results suggest that expression of mutant IFITM5 causes abnormal skeletal development, low bone mass, and abnormal osteoblast differentiation PMID: 25251575
  2. The purpose of the current study was to re-assess the topology, localization, and biochemical properties of BRIL and compare it to the osteogenesis imperfecta type V mutant in MC3T3 osteoblasts. PMID: 24715519
  3. These results indicate that the S-palmitoylation on IFITM5 promotes the interaction with FKBP11. PMID: 24058703
  4. Key roles for the Sp members and GLI2 that possibly cooperate to activate Bril when the promoter becomes demethylated. PMID: 23530031
  5. Natural antisense transcripts enhance bone formation by increasing sense IFITM5 transcription. PMID: 22884724
  6. Ifitm5 deficiency might have a greater effect on prenatal bone development. PMID: 20838829
  7. these results suggest that IFITM5 is involved not only in bone formation, but also in immune system activity. PMID: 21600883
  8. Bril is a novel osteoblast protein and showed a role in mineralization, possibly identifying a new regulatory pathway in bone formation. PMID: 18442316

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
CD225/Dispanin family
Tissue Specificity
Detected in embryonic bone (at protein level). Highly expressed in osteoblasts of adults and embryos. Expressed in primitive hemopoietic cells.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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