Recombinant Mouse Transmembrane protein 8A (Tmem8a)

Code CSB-CF875366MO
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Source in vitro E.coli expression system
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Product Details

Target Names
Tmem8a
Uniprot No.
Alternative Names
Pgap6; Tmem8; Tmem8a; Post-GPI attachment to proteins factor 6; GPI processing phospholipase A2; GPI-PLA2; M83 protein; Transmembrane protein 8; Transmembrane protein 8A
Species
Mus musculus (Mouse)
Expression Region
34-769
Target Protein Sequence
SNSKESEAGLVSEHFSQAPQKLSFYSWYGSTRLFHFRVPPDTVLLRWLLHVSQGSPSCTD EEITVHFRYGAPPVINPLGTSFPDNTLSHASFHIRALLSTLMLDNTSVNISHPAPGDWFL VAHLPPSSQKIQVKGFVPTCAYIFQPDMLVMRVVEVSTLEPDVPLPQTLLSYPSYLKIFV PEYTQELRLELQGCVSSVSPGCPVRVTVGATTLPRNFQRVLTCTGLAPSCHLLLSSPPWG RWLQVTFESLAEPHVTVGFTAKAVFTVCRPWSVTIHHLIQNNPNQTYDTSAIQLSQSAVH RDLGRSSRVDSGPFCLLNYPVLREDTDVVSVHFQPLNGAFVLVHSSMPSVMQLRLDTGMD SGGSFIIVLRTNKTEVTNGTLVAACVNAASPFLSFNTSLNCTTAFFQGYPMFLRASSHMA NLIMPFPETDNWYLSLQLVCPESPEDCEQAVVRVETILYLVPCLNDCGPYGQCLLLRRYG YVYAGCSCKAGWRGWSCTDNSTAQTVAQQRAAALLLTLSNLMFLAPIAISLHRSFLVEAS VYFYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGASTWVTILCMARLKTILKQV LLVLGTLVIAMSLQMDRRGIWNLMGPCVFAFVIMASMWIYRCGHRGQCYPTSWQRWVFYL LPGISMASVGIAMYTSMMTSDNYYYTHSIWHILLAGSAAFLLPPREEKAGSWACLQKFPC HYQICRNDRDELYTVT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchor proteins (GPI-AP). Has phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI. Required for the shedding of the GPI-AP TDGF1, but not CFC1, at the cell surface. Shedding of TDGF1 modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. Also indirectly involved in the translocation of RAC1 from the cytosol to the plasma membrane by maintaining the steady state amount of CAV1-enriched plasma membrane subdomains, stabilizing RAC1 at the plasma membrane.
Gene References into Functions
  1. PGAP6 plays a critical role in Nodal signaling modulation through CRIPTO shedding. PMID: 27881714
Subcellular Location
Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
Protein Families
TMEM8 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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