Recombinant Nitrosococcus oceani Lipid A export ATP-binding/permease protein MsbA(msbA)

Code CSB-CF660520NAAI
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Source in vitro E.coli expression system
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Product Details

Target Names msbA
Uniprot No. Q3J7R8
Alternative Names msbA; Noc_2675; ATP-dependent lipid A-core flippase; Lipid A export ATP-binding/permease protein MsbA
Species Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107)
Expression Region 1-597
Target Protein Sequence MTFTPSLNSGLAVYRRLLSYTRPYRWIFAASIITMAIYAATETGLAALMKPLMDGSFIER DPATIQIIPLLLIGLFVIRGGANFITQYGLKWVARRVVRDLREQMFCHLLALPARYYDQK ASGQLLAKLIYDVEQVSNAATDAILTIIRDSLTILGLLAWMAYLNGLLTLIILVTAPLIA LIIWWVSHRFRRISRKIQNSMGDVSQVAQETIEGHREVKIFGGQTYEAERFDQVNEQNRR QTMKMAATDAISQPVVQLIAVLGLAGVIHLATRESMLAQISVGTFISFITAMMLLLGPVK RLTKINGTLQRGIAAAQSIFGLLAETPEADRGQQSLRRARGAIRFEHLSFCYEPAKGPVL ENIDLEIKPYQTIALVGHSGSGKSTLVSLLARFYETTSGRILIDEMDIQTLRLTELRRQI ALVSQQIILFNDTIAHNIAYGSYQQTSKQDIIRAAEAAHAMEFINRLPDGLDTVIGEKGV LLSGGQRQRLAIARALLKDAPILILDEATASLDTEAERHIQAALETLMRQRTTLVIAHRL STVENADQIIVLHQGQIIERGTHSQLLARESHYAGLYRLQFRHSHEHVSPLSANVGL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Involved in lipopolysaccharide (LPS) biosynthesis. Translocates lipid A-core from the inner to the outer leaflet of the inner membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.
Subcellular Location Cell inner membrane; Multi-pass membrane protein.
Protein Families ABC transporter superfamily, Lipid exporter (TC 3.A.1.106) family
Database Links

KEGG: noc:Noc_2675

STRING: 323261.Noc_2675

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