Recombinant Pseudomonas aeruginosa Sigma factor AlgU negative regulatory protein (mucA)

Code CSB-CF334370EZX
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
mucA
Uniprot No.
Alternative Names
mucA; PA0763; Sigma factor AlgU negative regulatory protein
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
1-194
Target Protein Sequence
MSREALQETLSAVMDNEADELELRRVLAACGEDAELRSTWSRYQLARSVMHREPTLPKLD IAAAVSAALADEAAPPKAEKGPWRMVGRLAVAASVTLAVLAGVRLYNQNDALPQMAQQGT TPQIALPQVKGPAVLAGYSEEQGAPQVITNSSSSDTRWHEQRLPIYLRQHVQQSAVSGTE SALPYARAASLENR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Negative regulator of the sigma factor AlgU. Plays a role in the differentiation of P.aeruginosa into the alginate-producing form. Inactivation of mucA causes a switch from the non-mucoid to mucoid state resulting in constitutive expression of alginate biosynthetic genes.
Gene References into Functions
  1. mucA gene mutation influenced the virulence of Pseudomonas aeruginosa in P. aeruginosa pneumonia in COPD patients. PMID: 29957276
  2. Inactivation of mucA (the gene responsible for the mucoid phenotype) in PAO1 leads to p38alpha MAPK activation by both TLR2 and TLR5, as observed in the cystic fibrosis clinical mucoid isolate PACF508. PMID: 23068105
  3. Alginate biosynthesis in Pseudomonas aeruginosa is a highly regulated by algU and mucA genes. PMID: 22842599
  4. Alginate induction in PAO1 on Pseudomonas isolation agar with ammonium metavanadate was correlated with increased proteolytic degradation of MucA. PMID: 21631603
  5. Genotypic variants of the P. aeruginosa dominant strains can coexist in the chronic colonization in CF patients. These variants can be undetected by RFLP and they might present variable antibiotic susceptibility. PMID: 21194804
  6. MucA mutation correlates with the alginate production and phenotype of bacterial colonies. PMID: 20045914
  7. Physiological experiments confirmed that the isogenic mucA22 mutant is less resistant to osmotic stress than the parental PA01 wild-type strain, but only in the stationary phase of growth. PMID: 20174684
  8. AlgW-mediated MucA degradation required algB and rpoN in the kinB mutant. PMID: 19168621
  9. Binding of MucB to MucA inhibits MucA cleavage by AlgW. PMID: 19298369

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Subcellular Location
Cell membrane; Single-pass membrane protein.
Protein Families
RseA family
Database Links

KEGG: pae:PA0763

STRING: 208964.PA0763

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