Recombinant Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (Hcn3)

Code CSB-CF864350RA
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Source in vitro E.coli expression system
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Product Details

Target Names
Hcn3
Uniprot No.
Alternative Names
Hcn3; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Species
Rattus norvegicus (Rat)
Expression Region
1-780
Target Protein Sequence
MEEEARPAVGDGEAATPARETPPAAPAQARAASGGVPESAPEPKRRQLGTLLQPTVNKFS LRVFGSHKAVEIEQERVKSAGAWIIHPYSDFRFYWDLIMLLLMVGNLIVLPVGITFFKEE NSPPWIVFNVLSDTFFLLDLVLNFRTGIVVEEGAEILLAPRAIRTRYLRTWFLVDLISSI PVDYIFLVVELEPRLDAEVYKTARALRIVRFTKILSLLRLLRLSRLIRYMHQWEEIFHMT YDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPSDCWVSMNRMVNHSWGRQYSHA LFKAMSHMLCIGYGQQAPVGMPDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQ EKYKQVEQYMSFHKLPADTRQRIHEYYEHRYQGKMFDEESILGELSEPLREEIINFTCRG LVAHMPLFAHADPSFVTAVLTKLRFEVFQPGDLVVREGSVGRKMYFIQHGLLSVLARGAR DTRLTDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDHFNAVLEEFPMMRRAFETVAM DRLRRIGKKNSILQRKRSEPSPGSSSGGVMEQHLVQHDRDMARGIRGLAPGTGARLSGKP VLWEPLVHAPLQAAAVTSNVAIALTHQRGPLPLSPDSPATLLARSARRSAGSPASPLVPV RAGPLLARGPWASTSRLPAPPARTLHASLSRTGRSQVSLLGPPPGGGGRRLGPRGRPLSA SQPSLPQRATGDGSPRRKGSGSERLPPSGLLAKPPGTVQPSRSSVPEPVTPRGPQISANM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Hyperpolarization-activated potassium channel. May also facilitate the permeation of sodium ions.
Gene References into Functions
  1. The low-intensity HCN3 band density in trigeminal ganglion neurons increased with postnatal age P1-P35. PMID: 21753027
  2. Immunoreactivity for HCN3 in rat brain shows subcortical distribution mainly concentrated in the hypothalamus. PMID: 14991560
  3. Of particular importance is that we detected no currents upon hyperpolarization in the ST14A cells at the nonproliferative stage when only HCN3 protein was present. PMID: 16175581
  4. HCN3 mRNA was preferentially expressed in small diameter (20-30 microm) neurons and HCN3 are present in large diameter neurons and small diameter neurons. PMID: 18450385
  5. These data establish HCN3 up-regulation as a novel candidate mechanism that might contribute to the in vivo changes of electrical activity in basal ganglia output neurons of the parkinsonian brain. PMID: 19320057

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Potassium channel HCN family
Database Links
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