Recombinant Rat Prostaglandin E2 receptor EP2 subtype (Ptger2)

Code CSB-CF723483RA
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Ptger2
Uniprot No.
Alternative Names
Ptger2; Prostaglandin E2 receptor EP2 subtype; PGE receptor EP2 subtype; PGE2 receptor EP2 subtype; Prostanoid EP2 receptor
Species
Rattus norvegicus (Rat)
Expression Region
1-357
Target Protein Sequence
MDNSFNDSRRVENCESRQYLLSDESPAISSVMFTAGVLGNLIALALLARRWRGDTGCSAG SRTSISLFHVLVTELVLTDLLGTCLISPVVLASYSRNQTLVALAPESRACTYFAFTMTFF SLATMLMLFAMALERYLAIGHPYFYRRRVSRRGGLAVLPAIYGVSLLFCSLPLLNYGEYV QYCPGTWCFIQHGRTAYLQLYATVLLLLIVAVLGCNISVILNLIRMQLRSKRSRCGLSGS SLRGPGSRRRGERTSMAEETDHLILLAIMTITFAVCSLPFTIFAYMDETSSRKEKWDLRA LRFLSVNSIIDPWVFVILRPPVLRLMRSVLCCRTSLRAPEAPGASCSTQQTDLCGQL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Gene References into Functions
  1. EP2 contributes to generating mechanical hyperalgesia through positive feedback upregulation of COX-2 expression in muscle after lengthening contractions PMID: 28759126
  2. These results indicate that EP2 activation, which initially promotes microglial activation, later causes delayed death of activated microglia, potentially contributing to the resolution phase of neuroinflammation. PMID: 25715797
  3. In the spinal cord 1 day after painful joint injury there is increased expression of prostaglandin E2 receptor. PMID: 24253784
  4. Elastogenesis is spatially regulated by PGE-EP4 signaling in the ductus arteriosus. PMID: 24146253
  5. Data indicate that painful cervical facet joint distraction induces an immediate and sustained increase of prostaglandin E2 (PGE2) receptor EP2 expression in the dorsal root ganglion (DRG). PMID: 22789984
  6. EP2 receptor signaling pathways regulate classical activation of microglia PMID: 23404506
  7. EP2 receptors were expressed in forebrain neurons and were induced in endothelial cells after hypoxic-ischemic injury in the neonatal rat. Activation by misoprostol reduced the injury. PMID: 21939736
  8. EP(4), and to a lesser degree EP(2), receptors mediate the dilatory effect of PGE(2) in the craniovascular system in rats PMID: 20713561
  9. It is suggested that PGE(2) may regulate above inflammatory mediators in the activated microglia via EP2-cAMP signaling pathway in hypoxic conditions. PMID: 19712723
  10. EP2 receptor, cAMP, and PKA are critical components of the signaling cascade between mechanical strain and gap junction-mediated communication between osteocytes PMID: 12939279
  11. EP2 receptor-selective agonists stimulate new bone formation on trabecular, endocortical, and periosteal surfaces and enhances fracture healing PMID: 14606517
  12. EP2 xpression at implantation site may play an important role during embryo implantation. PMID: 16136483
  13. A short 3-min cerebral ischemia and a 3-min ischemia followed by a second lethal ischemia enhanced the expression of EP2 and EP4 receptors in CA1 pyramidal neurons of the hippocampus. PMID: 16437207
  14. This data should be able to contribute to understand the involvement of PGE(2) in normal brain function and its participation in neuroinflammatory processes. PMID: 16546360
  15. EP2 receptor stimulation triggers cyclic AMP production but also causes release of Ca2+ from intracellular stores. PMID: 17058034
  16. EP(2) and EP(4) receptor signaling inhibits ethanol-induced gastric mucosal injury through cancellation of collecting venule constriction by reducing LTC(4) production. PMID: 17947453
  17. EP(4) receptor subtype as the major mediator of PGE(2)-induced electrogenic ion secretion with a lesser induction through the EP(2) receptor subtype. PMID: 18684231
  18. PGE(2) receptor types exist in the retina of rat pups and remain functional when the retina is cultured. Activation of EP2 receptors with butaprost attenuates an insult caused by insufficient serum that may occur in situ by reduced trophic support. PMID: 19524109
  19. PGE2 action at microglial EP2 receptors inhibits the microglial production of the cholinergic differentiating cocktail. PMID: 19555672

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Database Links
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