Recombinant Xenopus laevis E3 ubiquitin-protein ligase RNF19B (rnf19b)

Code CSB-CF600935XBE
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Source in vitro E.coli expression system
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Product Details

Target Names
rnf19b
Uniprot No.
Alternative Names
rnf19b; E3 ubiquitin-protein ligase RNF19B; RING finger protein 19B
Species
Xenopus laevis (African clawed frog)
Expression Region
1-687
Target Protein Sequence
MRLRNDCLVRLLTSWFGIFCLYEMTEGSAEPPPCPGARRRRLLLSLPNVFPGRTRAAPEP SVPSPPPSPPPPPPPPVSVPPPPSSPGGSESLIECPLCLVRQPPEEIPELLSCRHRSCLR CLRQYLRIEICESRVNLRCPECAERLSPQHVRAILRDPLLTRKYEEFLLRRCLAADPDCR WCPAPDCGYAVIAYGCASCPKLTCEREGCRTEFCYHCKHVWHPNQTCDMARQQRAPSLGV RRKHPSGISYGQESGSADDMKSCPRCSAYIIKMNDGSCNHMTCSVCGCEFCWLCMKEISD LHYLSPSGCTFWGKKPWSRKKKIIWQLSTLIGAPVGISLIAGIAIPAMVIGIPVYVGRKI HGRFENKKTSRHKKNLAVTGGVILSVIASPVVAAVSVGIGVPIMLAYVYGVVPVSLCRGG GCGVTTANGKGVKIDFEEDGPITVADAWRALKNPSIGESSMEGLTSVLSTSGSPTDGLSV LQGNYSETASFAALAGGTLTGGMLSGGRAKYCRLEVQADVQKETCQKDSVSLGAVSDSAS TRAMAGSIISSYNPQEREVNNMEIQVHIEAKPSRYQLMSESSTEESLHASAPLVESEDAE ACRNQVAACDITLAQPESIRSDLESSDAQSDDVPDLASEEYDSPHLFPPSPSNALQESPP HRMCAQEEGLCAHEESLSKVEIIELRV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. Protects against staurosporin-induced cell death.
Subcellular Location
Cytoplasmic granule membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
RBR family, RNF19 subfamily
Database Links

UniGene: Xl.6482

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