Recombinant Xenopus laevis E3 ubiquitin-protein ligase synoviolin B (syvn1-b)

Code CSB-CF736440XBE
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
syvn1-b
Uniprot No.
Alternative Names
syvn1-b; hrd1-b; E3 ubiquitin-protein ligase synoviolin B; RING-type E3 ubiquitin transferase synoviolin B; Synovial apoptosis inhibitor 1-B
Species
Xenopus laevis (African clawed frog)
Expression Region
17-595
Target Protein Sequence
YYLKNQFYPTVVYLTKSSPSMAILYIQAFVLVFLLGKFMGKVFFGQLRAAEMEHLLERSW YAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLAEDRVDFMERSPNISWLFHFRIL ALMLLLGVLDAFFVSHAYNSLVTRGASVQLVFGFEYAILMTMILAVFIKYILHSVDLQSE NPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMVKVHTFPLFAIRPMYLAMRQFKKAVTDA VMSRRAIRNMNTLYPDATAEELQAMDNVCIICREEMVSGAKRLPCNHIFHTSCLRSWFQR QQTCPTCRMDVLRASLPTQPQTPAEQQNQHQAQQQPTPVVPPQPNFPPGMLPPFPPGMFP LWPPMGQFPPVPGAPVGNPPDEANPGSSSGSSARAGETSNVGSESHPGAALPGFPFPPPF LGMSILPPFGLPPMPMPPAGFAGLTDEELRAMEGHERQNLEARLQCLQNIHTLLDAAMLQ INQYLTVLASIGPPQPPVSSSSSSSASASTEPTTSSVSEPVIDTSSIVTTDSSQQSASPV PVNVETLGGAEGGETPTEEPDNVELRRRRLQKLETTDSQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins. Protects cells from ER stress-induced apoptosis. Sequesters p53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
HRD1 family
Database Links

KEGG: xla:494996

UniGene: Xl.64351

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