Recombinant Xenopus laevis Ephrin-B1 (efnb1)

Code CSB-CF007465XBE
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
efnb1
Uniprot No.
Alternative Names
efnb1; eplg2; lerk2; Ephrin-B1; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2; XlERK
Species
Xenopus laevis (African clawed frog)
Expression Region
21-329
Target Protein Sequence
LGKNLEPVTWNSQNPRFISGKGLVLYPEIGDRLDIICPKGDSSQPYEYYKLYMVRRDQLEACSTVIDPNVLVTCNQPGKEYRFTIKFQEFSPNYMGLEFRRNQDYYITSTSNSTLQGLENREGGVCQTRSMKIIMKVGQDPNAVPPEQLTTTRPSKEADNTGKIATFGPWNGPVENPGKSDTNLSDKPTAGGGVDGFFNSKIAVFAAIGAGCVIFILIIIFLVVLLIKIRKRHRKHTQQRAAALSLSTLASPKCSGNAGSEPSDIIIPLRTTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May have a role in the developing mesenchymal and nervous tissue.
Gene References into Functions
  1. interactions between Smurfs and ephrinB1 regulates the maintenance of tissue boundaries through the control of ephrinB protein levels PMID: 23475958
  2. Results identify the transcriptional co-repressor xTLE1/Groucho as an EphrinB1 interacting protein. PMID: 21429299
  3. Ephrin-B1 and Wnt3A signaling contribute to region-specific neuronal plasticity in the developing optic tectum. PMID: 20346764
  4. Tyrosine-298 in ephrin B1 is required for binding Grb4. PMID: 14535844
  5. EphrinB1 reverse signaling is required to promote cellular movements into the eye field, and can rescue the FGF receptor-induced repression of retinal fate. respectively. PMID: 14723847
  6. Gain- and loss-of-function experiments suggest that Xdsh associates with ephrinB1 and mediates ephrinB1 signalling through downstream members of the PCP pathway during eye field formation. PMID: 16362052
  7. recruitment of STAT3 to ephrinB1, and its resulting Jak2-dependent activation and transcription of reporter targets, reveals a signaling pathway from ephrinB1 to the nucle PMID: 17954917
  8. ephrinB1 associates with the Par polarity complex protein Par-6 and can compete with the small GTPase Cdc42 for association with Par-6. PMID: 18604196
  9. Data demonstrate that phosphorylation of tyrosines 324 and 325 of ephrin B1 disrupts the ephrinB1/Dishevelled interaction, thus modulating retinal progenitor movement that is dependent on the planar cell polarity pathway. PMID: 19005214

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Ephrin family
Tissue Specificity
Expressed at low levels in most tissues with highest levels in the kidney, oocytes, ovary and testis.
Database Links

KEGG: xla:394292

UniGene: Xl.302

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