Recombinant Xenopus laevis Vang-like protein 2-B (vangl2-b)

Code CSB-CF841936XBE
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
vangl2-b
Uniprot No.
Alternative Names
vangl2-b; stbm-a; Vang-like protein 2-B; Protein strabismus-B; Van Gogh-like protein 2-B; Xstrabismus-B; Xstbm-B
Species
Xenopus laevis (African clawed frog)
Expression Region
1-521
Target Protein Sequence
MDNDSQYSGYSYKSGQSRSSRKHRDRRERHRSKSREGSRGDKSVTIQAPGEPLLDNESTR GEDRDDNWGETTTVVTGTSEHSISHDDITRITKDMEDSAKLDCSRHLGVVIAGALALLSF LTPIAFMLLPQILWREDLEQCGTACEGLFISVAFKLLILLLGSWALFFRRPKAFFPRVFV FRALLMVLVFLLVVSYWLFYGVRILESRDKNYQGIVQYAVSLVDALLFVHYLAVVLLELR QLQPQFTIKVVRSTDGASRFYNIGHLSIQRVAVWILENYYHDFPVYNPALLNLPKSILSK KMSGFKVYSLGEENNTNNSTGQSRAVIAAAARRRDNSHNEYYYEEAEHERRVRKRKARLV VAVEEAFTHIKRLQDEDPKNPREIMDPREAAQAIFASMARAMQKYLRTTKQQPYHTMESI LQHLEFCITHDMTPKAFLERYLGPGPTIQYHKDRWLAKQWTLVSEEPVTNGLKDGVVFEL KRQDFSLVVSTKKIPFFKLSEEFVDPKSHKFVMRLQSETSV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Has a role in non-canonical Wnt/planar cell polarity (PCP) signaling; can recruit dvl/dsh and prickle from the cytoplasm to the plasma membrane. Acts in a PCP complex to regulate the polarized assembly of fibronectrin on the surface of the mesoderm during gastrulation. Regulates convergent extension in both dorsal mesoderm and neural tissue without affecting cell fate. Regulates neural fold closure during neurulation. May be required for cell surface localization of fzd3 and fzd6 in the inner ear.
Gene References into Functions
  1. The interaction and interdependence of Vangl2, VAMP1, aPKC and the stable microtubule cytoskeleton in the oocyte, shows that maternal Vangl2 and aPKC are required for specific oocyte asymmetries and vertebrate embryonic patterning. PMID: 21813572
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Vang family
Tissue Specificity
During gastrulation, broadly expressed in the dorsal region in both mesodermal and neural tissues. From the neurula stages, expressed throughout the neural tube. In tailbud stages, expression declines in the anterior notochord but remains strong in the po
Database Links

KEGG: xla:399177

UniGene: Xl.5981

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