RT-PCR Primers

CUSABIO provides RT-PCR amplification of a particular RNA sequence requires two PCR primers that are specific for the gene transcript of interest. The primer design should allow differentiation between the amplified product from cDNA and an amplified product derived from contaminating genomic DNA. The primer used for reverse transcription affects both the size and the specificity of the cDNA produced. Four kinds of CUSABIO RT-PCR Primers are commonly used in RT-PCR, each with specific advantages.