Mini Sample ELISA Kits — Evaluate Before You Buy

What Are Mini Sample ELISA Kits?

Mini sample ELISA kits are specialized immunoassay formats engineered to accurately quantify target proteins or biomarkers using significantly reduced sample volumes – as low as 25 μL – rather than the 100 μL or more typically required by standard ELISA formats. This capability is essential for researchers working with limited, irreplaceable, or precious biological samples, such as pediatric serum, tissue biopsies, cerebrospinal fluid (CSF), or serial samples from longitudinal studies.

The kits achieve this exceptional low-volume sensitivity primarily through the use of optimized antibody pairs, polymerized Horseradish Peroxidase (Poly-HRP) conjugated detection antibodies, and enhanced plate coatings. This technology significantly amplifies the detection signal by incorporating multiple enzyme molecules per antibody binding event, enabling robust signal generation even with minimal analyte present in tiny sample volumes.

Why Choose CUSABIO Mini Sample ELISA Kits?

Maximize Conservation of Precious Samples

Sample volume reduced by up to 75%. Enables continuous research with precious samples such as transgenic animals, clinical puncture fluids, and pediatric specimens.

Significantly Reduce Experimental Costs

Lower sample usage directly translates to reduced consumption of detection reagents, effectively cutting costly sample collection and reagent expenses.

Exceptional Performance Without Compromise

Rigorously validated, CUSABIO's Mini Sample ELISA kits match or surpass conventional 100μL kits in sensitivity, specificity, and precision.

Mini Sample vs. Traditional

Detection Parameters	CUSABIO Mini sample ELISA Kit (25-50μL)	Traditional ELISA Kit (100μL)
Sample Volume	50 μL	100 μL
Sample Consumption	Extremely Low (Saves 50%-75%)	Standard
Reagent Consumption	Reduced	Higher
Applicable Scenarios	Precious Samples	Standard Samples

Selected Mini Sample ELISA Kits

We have selected the most in-demand targets to launch our micro-volume ELISA kits. Certain products require sample volumes as low as 25 μL, with more targets continuously under development!

Mini Sample Mouse Interleukin 1β,IL-1β ELISA Kit

Code: CSB-E08054m-MS
Detection Range: 12.5 pg/mL-800 pg/mL
Sensitivity: 6.25 pg/mL
Sample Types: serum, plasma, cell culture supernates, tissue homogenates

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Mini Sample Mouse Interleukin 6,IL-6 ELISA Kit

Code: CSB-E04639m-MS
Detection Range: 15.6 pg/mL-1000 pg/mL
Sensitivity: 7.8 pg/mL
Sample Types: serum, plasma, cell culture supernates, tissue homogenates

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Mini Sample Mouse chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) ELISA kit

Code: CSB-E17286m-MS
Detection Range: 4.69 pg/mL-300 pg/mL.
Sensitivity: 2.345 pg/mL
Sample Types: serum, plasma, cell culture supernates, tissue homogenates

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Mini Sample Mouse Interleukin 17,IL-17 ELISA Kit

Code: CSB-E04608m-MS
Detection Range: 10.938 pg/mL-700 pg/mL
Sensitivity: 5.469 pg/mL
Sample Types: serum, plasma, cell culture supernates, tissue homogenates

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Mini Sample Mouse Interleukin 2,IL-2 ELISA kit

Code: CSB-E04627m-MS
Detection Range: 3.9 pg/mL-250 pg/mL
Sensitivity: 1.95 pg/mL
Sample Types: serum, plasma, cell culture supernates, tissue homogenates

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Mini Sample Mouse Interleukin 13,IL-13 ELISA Kit

Code: CSB-E04602m-MS
Detection Range: 7.8 pg/mL-500 pg/mL
Sensitivity: 3.9 pg/mL
Sample Types: serum, plasma, cell culture supernates, tissue homogenates

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CUSABIO Quality Commitment

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Rigorous Quality Control

Each kit is triple-checked against eight key standards to ensure reliable and reproducible results.

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Over 27,000 Global Citations

Our products have been widely adopted by top research institutions worldwide, with cumulative citations exceeding 27,000 citations. Their reliability has been validated through the most rigorous scientific practice.

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Professional Technical Support

Our dedicated scientific team provides expert technical support to address any experimental inquiries you may have.

Frequently Asked Questions

Will a 25μL sample loading volume affect detection sensitivity and accuracy? +

Depending on the characteristics and technical requirements of different targets, we employ tailored optimization strategies. While the standardized sample loading volume is 50μL, certain products require a two-fold dilution, resulting in an actual sample consumption of 25μL. Regardless of whether 25μL or 50μL is used, both options significantly reduce sample consumption compared to traditional 100μL kits.

Why are the sample loading volumes 25μL for some products and 50μL for others? +

We employ tailored optimization strategies based on the characteristics and technical complexity of different targets. Some products achieve a 25μL loading volume through more advanced technologies, while others currently utilize 50μL. Regardless of whether 25μL or 50μL is used, both options significantly reduce sample consumption compared to traditional 100μL kits.

Is the 25μL kit truly suitable for my limited sample volume? +

Absolutely! This is precisely why we developed this product series. The 25μL loading volume can save up to 75% of your precious samples, enabling research that was previously impossible due to insufficient sample availability.

Why is the current selection of detectable targets limited? +

Thank you for your interest! We are rolling out our micro-volume ELISA kits in phases, with the initial release carefully selected based on the most pressing market demands. Our team is actively developing additional targets. If you have specific requirements, please feel free to contact us—your feedback will directly influence our product development roadmap.

Is special equipment needed for low-volume pipetting? +

No, standard laboratory pipettes are sufficient. For optimal precision, we recommend using calibrated pipettes and low-retention tips, which are common in most labs.

How is the protocol of the mini sample ELISA kit different from that of a standard ELISA? +

The protocol is optimized but follows the same familiar steps. The key difference is the reduced volumes, which are clearly specified in the provided protocol to ensure ease of use.

Can I use the min sample ELISA kit if my sample volume is even lower than 25µL? +

For volumes below the recommended minimum, we advise consulting our technical support team for guidance on potential sample concentration strategies or custom solutions.

As low as 25μL, unlocking new heights in precise detection

What Are Mini Sample ELISA Kits?

Why Choose CUSABIO Mini Sample ELISA Kits?

Maximize Conservation of Precious Samples

Significantly Reduce Experimental Costs

Exceptional Performance Without Compromise

Mini Sample vs. Traditional

Selected Mini Sample ELISA Kits

Mini Sample Mouse Interleukin 1β,IL-1β ELISA Kit

Mini Sample Mouse Interleukin 6,IL-6 ELISA Kit

Mini Sample Mouse chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) ELISA kit

Mini Sample Mouse Interleukin 17,IL-17 ELISA Kit

Mini Sample Mouse Interleukin 2,IL-2 ELISA kit

Mini Sample Mouse Interleukin 13,IL-13 ELISA Kit

CUSABIO Quality Commitment

Rigorous Quality Control

Over 27,000 Global Citations

Professional Technical Support

Frequently Asked Questions