The Primer Pair is stable for up to one year at
-20° C in a non-frost free freezer.
Avoid repeated freeze-thaw cycles.
Human SFRP5 Primer Pair 2 for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.
Tested Applications :
Quality Control :
Primer Pairs for RT-PCR provide convenient primers for analyzing the expression of specific mRNAs or got specific PCR fragment by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 50μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR using human embryonic kidney 293T cells or human hepatic L02 cells total RNA.
PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.
Technical Hints :
• Thaw all reagents completely on ice before use.
• Use either random primers or oligo (dT) for reverse transcription.
• All PCR reactions should be assembled on ice.
• The recommended annealing