Baculovirus Expression Vector System (BEVS) is a kind of Eukaryotic expression system with high security. Due to a large genome so the BEVS can accommodate larger foreign gene, besides this, the recombinant viruses gained by BEVS can be easy to be screened. And it also has perfect post-translation modification system and efficient exogenous gene expression ability. BEVS is composed of transfer vector, baculovirus vector and host cells, which uses one or more baculovirus super promoter, and it can get recombinant baculovirus after the foreign target gene being inserted into the promoter. This recombinant baculovirus will replicate in insect cells and at the same time, the exogenous genes will get efficient expression.Baculovirus Expression Vector System (BEVS) has the following advantages compared with other expression vector systems.
•High Biologically Activity of Recombinant Protein: Baculoviruses can be propagated in insect hosts and the recombinant protein gained by BEVS can have a posttranslational modification in a manner similar to the mammalian cells. Most importantly, the expression product has strong biological activity.
|Insect cell expression system:|
Restructuring for constructing physical grain
By use of mating-assisted genetic integrated cloning (MAGIC)，we only need simple bacteria confusion, and then the recombinant baculoviruses vectors will be got rapidly, so a lot of time, energy and cost will be saved
30-50% off can be provided for renewal order
We can provide the detailed information of protein expression and purification, experimental data, as well as high titer of the virus liquid
Recombinant baculovirus carrier construction
Express conditions optimization
|Protein expression and purification|
|Insect cells protein expression items|
|1. protokaryon cloning||
||Nucleic acid electrophoresis images||Two weeks|
●PCR amplification product connected to pFastBac modified by our company
|●Get correct recombinant donor plasmid|
|2. xtensification training||
●Recombinant Baculoviruses obtain
●Recombinant Baculoviruses plasmid transfected insect cells
●High titer of the virus obtain
Plasmid DNA electrophoresis images with recombinant baculovirus vector,
|Three to four weeks|
|3. Optimization expression||
●High titer of the virus liquid infects insect cells again
|SDS-PAG, western blot||A half and one weeks|
|4. Protein expression and purification||●1L insect cells expression system and further purification||1L purified product of cell culture supernatant||One to two weeks|
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