CUSABIO Protein Expression Quotation Request Form

I.Your Information

  • Name*

    This field is required.

  • Email*

    This field is required.

  • Country*

    This field is required.

II.Product Information

  • Product Name (including species)*

    This field is required.

  • Uniprot No*

    This field is required.

  • Order Quantity

    This field is required.

  • Expression System

    This field is required.

  • Expression Region

    This field is required.

  • Sequence

    This sequence does not include the Tag sequence. Please fill if you have special demand.

  • Do you need tag ?

    Tag Removal Service

    If customer needs to remove the tag, please remark with tag removal service, otherwise, we won't remove the tag.

    There are extra charges for tag removal service. The cost will be list in the quotation.

    Not all protein tags can be removed as some proteins will be very unstable after tag removal.

    If we fail in removing the tag, we won’t charge for any extra cost, and remark this information in COA as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”

    Generally, the delivery time will be extended for 2-3 days.

    This field is required.

  • Molecular Weight

  • Application*

    This field is required.

III.Customer’s Requirement(Click here to show more)

  • Tag Removal Service

  • Endotoxin Level Requirement

  • Aseptic Manufacture Processing

  • Western Blot Service

  • Purity Requirement

  • Concentration Requirement

  • Storage Buffer Requirement

  • Delivery Form Requirement

  • Package Size Requirement

  • Other Requirement

Please refer to the following general statement for proteins before placing the order.

IV. General Statement

  • Tag Removal Service

    If customer needs to remove the tag, please remark with tag removal service, otherwise, we won't remove the tag.

    There are extra charges for tag removal service. The cost will be list in the quotation.

    Not all protein tags can be removed as some proteins will be very unstable after tag removal.

    If we fail in removing the tag, we won’t charge for any extra cost, and remark this information in COA as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”

    Generally, the delivery time will be extended for 2-3 days.

  • Endotoxin Removal Service

    If customer plans to use a protein in cell culture or has special requirement for endotoxin level, we recommend you to choose endotoxin removal service, it’s for free.

    We use conjugated PMB affinity filler adsorption to remove endotoxin and guarantee endotoxin level within 0.1ng / ug (1EU / ug).

  • Aseptic Manufacture Processing

    If customer plans to use a protein in cell culture, we recommend to choose aseptic manufacture processing when placing the order.

    We could offer aseptic manufacture processing service for free. The delivery time won’t be extended.

    The liquid proteins will be performed aseptic processing before lyophilization, but there may exist contamination during lyophilization process, so we can't guarantee bacteria free for the whole process.

    Aseptic manufacture processing will be shown in both of datasheet and COA as follows “0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, 50% glycerol”.

  • Western Blot Service

    We could offer Western Blot service for tag antibody free of charge. If customer has this demand, please remark this information when placing the order.

    Generally, the delivery time will be extended for 2-3 days.

    WB detection result will be shown in COA, but won’t be shown in datasheet.

  • Protein Purity

    We can guarantee the purity higher than 80% as determined by SDS-PAGE. The final actual purity will be shown in the COA.

  • Protein Concentration

    The protein concentration of each batch won't be exactly the same but we could guarantee 0.1-2.0 mg/ml concentration for E. coli and Yeast expressed proteins, and 0.1-0.5mg/ml for Baculovirus and Mammalian cell expressed proteins.

    If customer has special requirement for protein concentration, please remark your requirement in this form.

  • Storage Buffer

    The default storage buffer is 20mM Tris-HCl based buffer, pH8.0, 50% glycerol.

    If customer has special requirement for storage buffer, please remark when placing the order.

  • Storage Conditions

    For short-term storage, store at 4℃. For long-term storage, store at -20℃ or -80℃.

  • Protein Delivery Form

    The default delivery form is liquid form. If the customer has demand for lyophilized form, please remark this requirement when placing the order.

    Relatively speaking, the lyophilized form will be more stable than the liquid form, and the shelf life will be longer. But lyophilization may have damage to protein structure, and may affect activity of some proteins.

  • Buffer Before Lyophilization

    The buffer before lyophilization for different proteins will be different. Please ask the buffer composition each time.

  • Reconstitution

    The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins.(Dissolve completely. The concentration could depend on customers' experiment demand.)

    It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration. If customer has special requirements for concentration, then they could dilute the protein according to their experiment demand.

    We haven’t studied whether the optimal concentration range will vary among different hosts such as E.coli, yeast, mammalian or baculovirus, so we can’t provide useful advice.

    If customer will use immediately after dissolving one protein, then they don't need to add glycerol. If they won't use the protein in short term, we suggest to add some glycerol for storage. We suggest customer to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder. You could remark "The lyophilized powder should be packed into multiple vials" when placing order. The amount of lyophilized powder in each vial will depend on customer's experiment demand.

    In principle, the final concentration of glycerol from 5% to 50% all will be OK. Our default final concentration of glycerol is 50%. Customers could use it as reference.

  • Protein Shelf Life

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.

    So the shelf life of different proteins won't be exactly the same. We haven’t performed detailed research for this at present.

    Relatively speaking, the lyophilized form will be more stable than liquid form, and the shelf life will be longer. But lyophilization may have damage to protein structure, and may affect activity of some proteins.

    The shelf life of liquid form protein is 6 months around at -20℃/-80℃.

    The shelf life of lyophilized form is 12 months around at -20℃/-80℃.

    The shelf life of reconstituted recombinant protein is 6 months around at -20℃/-80℃. It refers that customer reconstitutes protein immediately using sterile water once receiving the item, and then add glycerol with certain final concentration, and finally store at -20℃/-80℃, and then the reconstituted protein can be stored for six months. If customers use the protein immediately, then they don't need to add glycerol, and the protein can be stored for about one week at 4℃.

    The above shelf life are concluded by our current storage experience. The specific shelf life will depend on specific protein.

  • Notes

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.