The cluster differentiation 276 (CD276), also known as B7-H3, is an immune checkpoint molecule of the B7-CD28 family. CD276/B7-H3 is lowly expressed on normal healthy tissues and activated cells of the immune system but is aberrantly overexpressed in various types of cancer. Increased expression of CD276/B7-H3 is correlated with growth, metastasis, invasion as well as poor clinical prognosis of tumors.
CD276/B7-H3 contains a putative 28 amino acid signal peptide, a 217 amino acid extracellular region harboring immunoglobulin constant (IgC) and variable (IgV) structures, a transmembrane region, and a 45 amino acid cytoplasmic domain.
Murine CD276/B7-H3 consists of a signal extracellular IgV-IgC domain and a signature intracellular 2Ig-B7-H3 domain, while CD276/B7-H3 in human 4Ig-B7-H3 that consists of nearly exact tandem duplication of the IgV-IgC domain. The intracellular tail of CD276/B7-H3 is short and has no known signaling motif.
CD276/B7-H3 plays a dual role in regulating innate immune responses. For example, CD276/B7-H3 expressed on the neuroblastoma cells protect them from NK cell-mediated lysis. Whereas, CD276/B7-H3 costimulates innate immunity by augmenting proinflammatory cytokines release from LPS-stimulated monocytes/macrophages, in both a Toll-like receptor 4- and 2-dependent manner. In terms of adaptive immunity, the function of CD276/B7-H3 is controversial. Human CD276/B7-H3 functions as a costimulatory molecular and augments TCR-mediated T-cell proliferation, IFNγ production, and generation of CTLs in vitro. Contrastly, mouse CD276/B7-H3 acts as a co-inhibitor and suppresses Th1-mediated immune response in airway inflammation and EAE development.
With a preferential expression on tumor cells, CD276/B7-H3 is an attractive target for cancer immunotherapy. The CD276/B7-H3 protein, especially when located in the cell membrane, may be an ideal choice for targeted drug development.
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Recombinant Human CD276 antigen(CD276), partial (Active) (C-terminal FC-Myc-tagged) (CSB-MP733578HU)
Validated Data
Greater than 93% as determined by SDS-PAGE.
Immobilized CD276 at 2 μg/ml can bind Anti-CD276 rabbit monoclonal antibody, the EC50 of human CD276 protein is 1.961-2.243 ng/ml.
The following Cd276 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Cd276 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004912GA01HU | CD276 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA005320 | CD276 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA745871ESR1HU | CD276 Antibody |
Human | ELISA, WB, IHC |
CSB-PA745871ESR2HU | CD276 Antibody |
Human | ELISA, IHC |
Cd276 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP731567XBE CSB-EP731567XBE CSB-BP731567XBE CSB-MP731567XBE CSB-EP731567XBE-B |
Recombinant Xenopus laevis CD276 antigen homolog (cd276), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Cd276 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP733578HU CSB-EP733578HU CSB-BP733578HU CSB-EP733578HU-B |
Recombinant Human CD276 antigen (CD276), partial |
Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
CSB-MP733578HU | Recombinant Human CD276 antigen (CD276), partial (Active) |
Mammalian cell |
Cd276 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP745871RA CSB-EP745871RA CSB-BP745871RA CSB-MP745871RA CSB-EP745871RA-B |
Recombinant Rat CD276 antigen (Cd276), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Cd276 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP848693MO CSB-EP848693MO CSB-BP848693MO CSB-MP848693MO CSB-EP848693MO-B |
Recombinant Mouse CD276 antigen (Cd276), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Cd276 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E14285h | Human Soluble CD276,sCD276/sB7-H3 ELISA Kit |
serum, plasma, urine, cell culture supernates | 0.78 ng/mL |