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Cathepsin W is a protein in humans that is encoded by CTSW gene. May have a specific function in the mechanism or regulation of T-cell cytolytic activity.
The following Ctsw reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Ctsw Antibodies for Mus musculus (Mouse)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA006205ZA01MO | Ctsw Antibody |
Mus musculus | ELISA, WB (ensure identification of antigen) |
Ctsw Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP006205HU CSB-BP006205HU CSB-MP006205HU CSB-EP006205HU-B |
Recombinant Human Cathepsin W (CTSW) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Ctsw Proteins for Mus musculus (Mouse)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP006205MO CSB-BP006205MO CSB-MP006205MO CSB-EP006205MO-B |
Recombinant Mouse Cathepsin W (Ctsw) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Ctsw Proteins for Felis catus (Cat) (Felis silvestris catus)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP006205CA CSB-EP006205CA CSB-BP006205CA CSB-MP006205CA CSB-EP006205CA-B |
Recombinant Cat Cathepsin W (CTSW) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Ctsw Proteins for Mus musculus(Mouse)
| Code | Product Name | Source |
|---|---|---|
| CSB-EP006205MO | Recombinant Mouse Cathepsin W (Ctsw) |
E.coli |
Cathepsin W, a cysteine endopeptidase of the papain family, is encoded by the CTSW gene that mapped to 11q13.1 [1]. The CTSW gene is tightly linked to cathepsin F, on human chromosome 11q13 [2]. So CTSW is highly similar to CTSF. CTSW consists of a putative 21-residue signal peptide, a 106-residue propeptide, and a 252-residue mature protein [3]. A 21-aa insertion between the His291 (numbering from the Met start residue) and Asn331 that contains a serine-proline-glutamine rich stretch (SSQSQPQPP) in CTSW makes it unique in the cathepsins. CTSW contains a fourth disulfide bond that is absent in other cathepsins, as well as an “orphan” cysteine (Cys132) capable of contributing to dimerization [4]. The 8-aa C-terminal extension in CTSW is also not found in other cathepsins [3]. CTSW is mainly expressed in CD8+ lymphocytes and natural killer (NK) cells [5] and may exert a role in cell-mediated cytotoxicity [3][6]. The expression of CTSW is upregulated by interleukin (IL)-2 [7]. CTSW is detected in up to 65% of CD45+ cells in autoimmune gastritis [8]. In contrast, the number of CTSW-expressing cells in Crohn's disease and ulcerative colitis is much lower [8]. These differences imply a distinct involvement of cytotoxic cells expressing CTSW in the pathogenesis among these diseases.
[1] Wex T, Levy B, et al. Genomic structure, chromosomal localization, and expression of human cathepsin W [J]. Biochem Biophys Res Commun. 1998 Jul 20; 248(2):255-61.
[2] Wex, T., Buhling, F., et al. Human Cathepsins F and W: A New Subgroup of Cathepsins [J]. Biochem. Biophys. Res. Commun. 1999, 259, 401-407.
[3] Linnevers, C., Smeekens, S. P., et al. Human Cathepsin W, a Putative Cysteine Protease Predominantly Expressed in CD8+ T-lymphocytes [J]. FEBS Lett. 1997, 405, 253-259.
[4] Brinkworth, R. I., Tort, J. F., et al. Phylogenetic Relationships and Theoretical Model of Human Cathepsin W (Lymphopain), a Cysteine Proteinase From Cytotoxic T Lymphocytes [J]. Int. J. Biochem. Cell Biol. 2000, 32, 373-384.
[5] T. Wex, H. Wex, R. Hartig, et al. Functional involvement of cathepsin W in the cytotoxic activity of NK-92 cells [J]. FEBS Lett., 552 (2003), pp. 115-119.
[6] Wex T, Bühling F, et al. Human cathepsin W, a cysteine protease predominantly expressed in NK cells, is mainly localized in the endoplasmic reticulum [J]. J Immunol. 2001 Aug 15; 167(4):2172-8.
[7] Thomas Wex, Frank Bühlin, et al. Human Cathepsin W, a Cysteine Protease Predominantly Expressed in NK Cells, Is Mainly Localized in the Endoplasmic Reticulum [J]. J Immunol August 15, 2001, 167 (4) 2172-2178.
[8] Frank Buhling, Udo Kellner, et al. Characterization of Novel Anti-Cathepsin W Antibodies and Cellular Distribution of Cathepsin W in the Gastrointestinal Tract [J]. Biol. Chem. 2002, 383, 1285-1289.
