Ghr

GHR is a gene encoding a protein named growth hormone receptor (also abbreviated as GHR). GHR protein belongs to the class I cytokine receptor family that is widely expressed in GH target cells. The first step for GH to act at the tissue and cell level is to bind to GHR on the surface of the target cell, and GHR mediates the signal transmission into cells to activate diverse signal cascades, leading to growth promotion, cell division, and regeneration.

What is the Structure of GHR?

GHR is a single-chain transmembrane glycoprotein consisting of three regions, including extracellular region (245aa), transmembrane region (25aa) and intracellular region (350aa). In the extracellular region, there are five conserved glycosylation sites, which are the sites for ligand binding. Near the cell membrane, there is a WSXWS-like motif in the extracellular domain , which is a conserved sequence consisting of 5 amino acid residues such as Trp-Ser-XXX-Trp-Ser (XXX represents any amino acid). This structure may play a role in stabilizing the spatial conformation during the binding of GH to GHR. In the intracellular region, two conserved sequences have been found, a sequence close to the cell membrane-sequence box I (Box1) consists of 8 amino acids and is rich in proline. Another segment-sequence box 2 (Box2) contains 15 amino acids and is 30 amino acid residues away from Box I.

What are Signaling Pathways Meditating by GHR?

GHR belongs to the cytokinin/hematopoietin receptor superfamily. When GH binds to GHR, GHR undergoes a dimerization process, and finally one GH binds to two GHRs. The signal transduction pathways mediated by GHR mainly include: tyrosine kinase (JKA2) system and protein kinase C (PKC) pathway. Additionally, emerging evidence also revealed that GHR can also signal through the IRS pathway. And nutritional status and endocrine factors such as GH also have regulatory effects on the expression of GHR.

Popular Product

● Recombinant Human Growth hormone receptor(GHR),partial (Active) (CSB-MP009411HU)

Validated Data

High Purity Validated by SDS-PAGE

Greater than 93% as determined by SDS-PAGE.

Excellent Bioactivity Validated by Functional ELISA

Immobilized GH1 (CSB-MP009407HU) at 1 μg/ml can bind human GHR, the EC₅₀ of human GHR protein is 24.96-33.39 ng/ml.

High Bioactivity Validated by LSPR

Human GH1 protein his/myc tag (CSB-MP009407HU) captured on COOH chip can bind Human GHR protein Fc tag with an affinity constant of 6.1 nM as detected by LSPR Assay.

● Recombinant Human Growth hormone receptor(GHR),partial,Biotinylated (Active) (CSB-MP009411HUj1-B)

Validated Data

Purity determined by SDS-PAGE

Greater than 95% as determined by SDS-PAGE.

Excellent Bioactivity Validated by Functional ELISA

Immobilized human GH1 (CSB-MP009407HU) at 2 μg/ml can bind Biotinylated human GHR, the EC₅₀ is 2.067-3.208 ng/ml.