Nucleoprotein Proteins

The coronavirus nucleocapsid (N) is a structural protein that forms complexes with genomic RNA, interacts with the viral membrane protein during virion assembly and plays a critical role in enhancing the efficiency of virus transcription and assembly.[1] It is the most abundant protein in coronavirus and often used as a marker in diagnostic assays. CUSABIO started the research of nucleoprotein at the beginning of SARS outbreak. And the related products have been manufactured since the beginning of February 2020.

CUSABIO provides 29 recombinant Nucleoprotein of mutiple species, including BCoV, HCoV-229E, HCoV-OC43 and SARS-CoV-2 etc. 6 of them can be used for SARS-CoV-2/ 2019-nCoV research use. The expression systems include E.coli, Mammalian cell, Yeast, and Baculovirus. Two of them have a purity of more than 90%. The other two of them have a purity of more than 85%. Moreover, CUSABIO 100% offers Risk-free Performance Guarantee. So you will have no worries.

Features of SARS-CoV-2 Nucleoprotein Proteins by CUSABIO

Nucleoprotein Proteins Catalog

Nucleoprotein Proteins for Bovine coronavirus (strain Mebus) (BCoV)(BCV)

Nucleoprotein Proteins for Human coronavirus 229E (HCoV-229E)

Nucleoprotein Proteins for Human coronavirus OC43 (HCoV-OC43)

Nucleoprotein Proteins for Human coronavirus NL63 (HCoV-NL63)

Nucleoprotein Proteins for Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Nucleoprotein Proteins for Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Nucleoprotein Background

A new coronavirus, SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), has recently emerged to cause a human pandemic [2]. About 2 to 14 days after exposure, the COVID-19 patients mainly manifest fever, cough, shortness of breath or difficulty breathing, chills, accompanied by severe pulmonary infection. After the virus is sequenced, nucleic acid tests have been used to detect SARS-CoV-2 infection. Nasopharyngeal swab nucleic acid tests detect viral RNA and indicate current viral infection, and they are an essential part of contact tracing and testing [3]. However, they are far not to meet the huge demand for the PCR primers and global supply challenges. Berend-Jan Bosch, a coronavirus specialist at Utrecht University in the Netherlands, said that all viral proteins will elicit antibody responses to some extent. And Peng Zhou, who leads the bat virus infection and immunity group at the Wuhan Institute of Virology in China, said that since the nucleocapsid protein is the most abundant viral protein, it's easy to detect. But spike protein is very specific for the new coronaviruses. In response to the viral proteins, the immune system triggers the production of antibodies that flag or neutralize the virus. So neutralizing antibodies of spike protein and nucleocapsid can be an indicator for the SARS-CoV-2. Based on these factors, scientific researchers developed serological assays for the detection of SARS-CoV-2 neutralizing, spike- and nucleocapsid-specific antibodies. Package of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) forms the nucleocapsid of the SARS-CoV-2. People commonly call it N protein. The nucleocapsid is a structural protein complexed with genomic RNA, and it interacts with the viral membrane protein during virion assembly. N protein is important in enhancing the efficiency of virus transcription and assembly [1]. Coronavirus N proteins have three distinct and highly conserved domains: the N terminal domain (NTD/domain 1) and the C-terminal domain (CTD/domain 3), which are separated by an intrinsically disordered central region (RNA-binding domain/domain 2) [1]. Besides, N protein plays a crucial role in viral pathogenesis. Theoretically, the viral antigen is the specific marker of the virus and appears before the corresponding antibody within the contracted population. Some experiments have demonstrated that nucleocapsid protein can indicate the presence of COVID-19.

[1] Ruth McBride, Marjorie van Zyl, et al. The Coronavirus Nucleocapsid Is a Multifunctional Protein [J]. Viruses. 2014 Aug; 6(8): 2991–3018.
[2] Zhou P, Yang XL, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020 Mar;579(7798):270-3.
[3] Corman VM, Landt O, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro surveillance : bulletin Europeen sur les maladies transmissibles European communicable disease bulletin. 2020 Jan;25(3):2000045.


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