Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.
The following Spike glycoprotein reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Spike glycoprotein Antibodies for Human coronavirus OC43
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA336163EA01HIY | S Antibody |
Human coronavirus OC43 | ELISA |
Spike glycoprotein Antibodies for Bovine coronavirus
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA333052LA01BJO | S Antibody |
Bovine coronavirus | ELISA |
CSB-PA333052LC01BJO | S Antibody, FITC conjugated |
Bovine coronavirus | |
CSB-PA333052LD01BJO | S Antibody, Biotin conjugated |
Bovine coronavirus | ELISA |
Spike glycoprotein Antibodies for Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-RA33245A0GMY | S Recombinant Monoclonal Antibody |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA, GICA |
CSB-RA33245A1GMY | S Recombinant Monoclonal Antibody |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA, GICA, Neutralising |
CSB-RA33245D1GMY | S Recombinant Monoclonal Antibody, Biotin conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-RA33245C1GMY | S Recombinant Monoclonal Antibody, FITC conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | |
CSB-RA33245B1GMY | S Recombinant Monoclonal Antibody, HRP conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-RA33245A2GMY | SARS-CoV-2 Spike RBD Recombinant Nanobody |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA, GICA, Neutralising |
CSB-EAP33245 | SARS-CoV-2 Spike RBD Antibody Pair 1 |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | S-ELISA |
CSB-RA33245D2GMY | SARS-CoV-2 Spike RBD Recombinant Nanobody, Biotin conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-RA33245C2GMY | SARS-CoV-2 Spike RBD Recombinant Nanobody, FITC conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-RA33245B2GMY | SARS-CoV-2 Spike RBD Recombinant Nanobody, HRP conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-MA33245A2m | S Monoclonal Antibody |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-MA33245A1m | S Monoclonal Antibody |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-MA33245A0m | S Monoclonal Antibody |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-PA33245YA01GMY | S Antibody |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-PA33245YD01GMY | S Antibody, Biotin conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-PA33245YA11GMY | S Antibody |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-PA33245YB11GMY | S Antibody, HRP conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-PA33245YC11GMY | S Antibody, FITC conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | |
CSB-PA33245YD11GMY | S Antibody, Biotin conjugated |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ELISA |
CSB-EAP33255A1 | SARS-CoV-2 N Antibody Pair 1 |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | S-ELISA |
CSB-EAP33255A2 | SARS-CoV-2 N Antibody Pair 2 |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | S-ELISA |
CSB-EAP33255A3 | SARS-CoV-2 N Antibody Pair 3 |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | S-ELISA |
CSB-EAP33255A4 | SARS-CoV-2 N Antibody Pair 4 |
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | S-ELISA |
Spike glycoprotein Antibodies for Human coronavirus HKU1 (isolate N5) (HCoV-HKU1)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA607323ZA01HIW | N Antibody |
Human coronavirus HKU1 | ELISA, WB (ensure identification of antigen) |
Spike glycoprotein Antibodies for Human coronavirus 229E
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA321366ZA01HIT | S Antibody |
Human coronavirus 229E | ELISA, WB |
Spike glycoprotein Proteins for Bovine coronavirus (strain vaccine) (BCoV) (BCV)
Code | Product Name | Source |
---|---|---|
CSB-EP333052BJO | Recombinant Bovine coronavirus Spike glycoprotein (S), partial |
E.coli |
CSB-YP333052BJO | Recombinant Bovine coronavirus Spike glycoprotein (S), partial |
Yeast |
CSB-BP333052BJO CSB-MP333052BJO CSB-EP333052BJO-B |
Recombinant Bovine coronavirus Spike glycoprotein (S), partial |
Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP333052BJOc7 | Recombinant Bovine coronavirus Spike glycoprotein (S), partial |
E.coli |
Spike glycoprotein Proteins for Human coronavirus OC43 (HCoV-OC43)
Code | Product Name | Source |
---|---|---|
CSB-MP336163HIY CSB-EP336163HIY-B |
Recombinant Human coronavirus OC43 Spike glycoprotein (S), partial |
Mammalian cell In Vivo Biotinylation in E.coli |
CSB-YP336163HIY | Recombinant Human coronavirus OC43 Spike glycoprotein (S), partial |
Yeast |
CSB-EP336163HIY | Recombinant Human coronavirus OC43 Spike glycoprotein (S), partial |
E.coli |
CSB-EP336163HIY1 | Recombinant Human coronavirus OC43 Spike glycoprotein (S), partial |
E.coli |
CSB-BP336163HIY | Recombinant Human coronavirus OC43 Spike glycoprotein (S), partial |
Baculovirus |
CSB-MP336163HIY1 | Recombinant Human coronavirus OC43 Spike glycoprotein (S), partial |
Mammalian cell |
Spike glycoprotein Proteins for Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Code | Product Name | Source |
---|---|---|
CSB-EP348663HQE CSB-BP348663HQE CSB-EP348663HQE-B |
Recombinant Human SARS coronavirus Spike glycoprotein (S), partial |
E.coli Baculovirus In Vivo Biotinylation in E.coli |
CSB-MP348663HQE | Recombinant Human SARS coronavirus Spike glycoprotein (S), partial (Active) |
Mammalian cell |
CSB-YP348663HQE | Recombinant Human SARS coronavirus Spike glycoprotein (S), partial |
Yeast |
CSB-YP348663HQEa4 | Recombinant Human SARS coronavirus Spike glycoprotein (S), partial |
Yeast |
Spike glycoprotein Proteins for Human coronavirus HKU1 (isolate N5) (HCoV-HKU1)
Code | Product Name | Source |
---|---|---|
CSB-YP607323HIW CSB-BP607323HIW CSB-EP607323HIW-B |
Recombinant Human coronavirus HKU1 Nucleoprotein (N) |
Yeast Baculovirus In Vivo Biotinylation in E.coli |
CSB-EP607323HIW | Recombinant Human coronavirus HKU1 Nucleoprotein (N) |
E.coli |
CSB-EP607323HIWc7 | Recombinant Human coronavirus HKU1 Nucleoprotein (N) |
E.coli |
CSB-MP607323HIW | Recombinant Human coronavirus HKU1 Nucleoprotein (N) |
Mammalian cell |
Spike glycoprotein Proteins for Human coronavirus NL63 (HCoV-NL63)
Code | Product Name | Source |
---|---|---|
CSB-EP747509HIX CSB-BP747509HIX CSB-MP747509HIX CSB-EP747509HIX-B |
Recombinant Human coronavirus NL63 Spike glycoprotein (S), partial |
E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-YP747509HIX1 | Recombinant Human coronavirus NL63 Spike glycoprotein (S), partial |
Yeast |
CSB-YP747509HIX | Recombinant Human coronavirus NL63 Spike glycoprotein (S), partial |
Yeast |
Spike glycoprotein Proteins for Human coronavirus 229E (HCoV-229E)
Code | Product Name | Source |
---|---|---|
CSB-YP321366HIT CSB-BP321366HIT CSB-MP321366HIT CSB-EP321366HIT-B |
Recombinant Human coronavirus 229E Spike glycoprotein (S), partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP321366HIT | Recombinant Human coronavirus 229E Spike glycoprotein (S), partial |
E.coli |
CSB-MP321366HIT1 | Recombinant Human coronavirus 229E Spike glycoprotein (S), partial |
Mammalian cell |
Spike glycoprotein Proteins for Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Spike glycoprotein Proteins for Severe acute respiratory syndrome coronavirus (SARS-CoV)
Code | Product Name | Source |
---|---|---|
CSB-MP348663HQEc7 | Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial |
Mammalian cell |
Spike glycoprotein ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL33241HU | Human SARS-CoV-2 S1 RBD IgG Antibody ELISA Kit |
serum, plasma | |
CSB-EL33242HU | Human SARS-CoV-2 S1 RBD IgM Antibody ELISA Kit |
serum, plasma |