Inhibitor of nuclear factor kappa-B kinase subunit alpha is a protein in humans that is encoded by CHUK gene. Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis.
The following chuk reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
chuk Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA916377 | Phospho-CHUK (Thr23) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA904221 | CHUK (Ab-23) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA586587 | Phospho-CHUK/IKBKB (Ser180/181) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA983649 | Phospho-CHUK/IKBKB (Ser176/177) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA000550 | Phospho-CHUK (T23) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA003008 | CHUK Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA974724 | CHUK Antibody |
Human,Mouse | ELISA,WB,IHC |
chuk Antibodies for Danio rerio (Zebrafish) (Brachydanio rerio)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA676836XA01DIL | chuk Antibody |
Danio rerio (Zebrafish) (Brachydanio rerio) | ELISA, WB (ensure identification of antigen) |
chuk Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP005420HU CSB-EP005420HU CSB-BP005420HU CSB-MP005420HU CSB-EP005420HU-B |
Recombinant Human Inhibitor of nuclear factor kappa-B kinase subunit alpha (CHUK) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
chuk Proteins for Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Code | Product Name | Source |
---|---|---|
CSB-YP637943XBF CSB-EP637943XBF CSB-BP637943XBF CSB-MP637943XBF CSB-EP637943XBF-B |
Recombinant Xenopus tropicalis Inhibitor of nuclear factor kappa-B kinase subunit alpha (chuk), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
chuk Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)
Code | Product Name | Source |
---|---|---|
CSB-YP676836DIL CSB-EP676836DIL CSB-BP676836DIL CSB-MP676836DIL CSB-EP676836DIL-B |
Recombinant Danio rerio Inhibitor of nuclear factor kappa-B kinase subunit alpha (chuk), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
chuk Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP713884MO CSB-EP713884MO CSB-BP713884MO CSB-MP713884MO CSB-EP713884MO-B |
Recombinant Mouse Inhibitor of nuclear factor kappa-B kinase subunit alpha (Chuk) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
chuk Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP719996CH CSB-EP719996CH CSB-BP719996CH CSB-MP719996CH CSB-EP719996CH-B |
Recombinant Chicken Inhibitor of nuclear factor kappa-B kinase subunit alpha (CHUK), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
chuk Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP740092XBE CSB-EP740092XBE CSB-BP740092XBE CSB-MP740092XBE CSB-EP740092XBE-B |
Recombinant Xenopus laevis Inhibitor of nuclear factor kappa-B kinase subunit alpha (chuk), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
chuk Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP836097BO CSB-EP836097BO CSB-BP836097BO CSB-MP836097BO CSB-EP836097BO-B |
Recombinant Bovine Inhibitor of nuclear factor kappa-B kinase subunit alpha (CHUK), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Conserved helix-loop-helix ubiquitous kinase, abbreviated for CHUK, is also called inhibitor of nuclear factor kappa-B kinase subunit alpha (IKK-α/IKKA) or IKK1 [1]. IKK-α, a serine⁄threonine kinase, belongs to the catalytic component of the inhibitor of the NF-kappa B Kinase(IKK) complex that plays a central role in regulating the NF-κB transcription factor [2]. Under homeostatic conditions, NF-κ B is weakly active due to cytoplasmic sequestration via noncovalent binding to IκB (inhibitor of NF-κB) proteins [3]. An NF-κB-inducing kinase (NIK), a central signaling component of the noncanonical NF-κB pathway, is targeted for continuous degradation by a tumor necrosis factor (TNF) receptor-associated factor-3 (TRAF3)-dependent E3 ubiquitin ligase [4]. Stimulated by non-canonical signals, NIK is stabilized from proteolysis to the extent that it becomes capable of recruiting and inducing the kinase activity of IKK-α [5] Subsequently, IKK-α and NIK phosphorylate three serines of NF-κB2/β100 within its C-terminal IκBδ segment, leading to the processing of NF-κB2 precursor protein p100 to p52. Phosphorylation-dependent ubiquitylation and proteasomal degradation of the IκB proteins lead to the release of active NF-κB dimers [6]. In turn, free NF-κB is translocated into the nucleus and activates the transcription of multiple genes involved in immune response, growth control, or protection against apoptosis. Since weak IKK- α activity has been reported in a large percentage of human squamous cell carcinomas, Lahtela J et al. found that normalizing IKK-α in mouse models of skin cancer exhibited an anti-tumorigenic effect [7]. IKK-α mutations in humans have been associated with lethal fetal malformations, which are characterized by shiny, thickened skin and truncated limbs in appearance [8].
[1] Mock BA, Connelly MA, et al. CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human chromosome 10 and mouse chromosome 19 [J]. Genomics. 1995, 27 (2): 348-51.
[2] Häcker H, Karin M. Regulation and function of IKK and IKK-related kinases [J]. Sci. STKE. 2006 (357): re13.
[3] Hinz M, Scheidereit C The IκB kinase complex in NF-κB regulation and beyond [J]. EMBO Rep. 2014 Jan; 15(1):46-61.
[4] Qing G, Qu Z, Xiao G Stabilization of basally translated NF-kappaB-inducing kinase (NIK) protein functions as a molecular switch of processing of NF-kappaB2 p100 [J]. J Biol Chem. 2005 Dec 9; 280 (49):40578-82.
[5] Vallabhapurapu S, Matsuzawa A, et al. Nonredundant and complementary functions of TRAF2 and TRAF3 in a ubiquitination cascade that activates NIK-dependent alternative NF-kappaB signaling [J]. Nat Immunol. 2008 Dec; 9(12):1364-70.
[6] Hayden MS, Ghosh S Shared principles in NF-kappaB signaling [J]. Cell. 2008 Feb 8; 132(3):344-62.
[7] Liu B, Park E, et al. A critical role for IκB kinase α in the development of human and mouse squamous cell carcinomas [J]. Proc. Natl. Acad. Sci. U.S.A. 2006, 103 (46): 17202-7.
[8] Lahtela J, Nousiainen HO, et al.Mutant CHUK and severe fetal encasement malformation [J]. New England Journal of Medicine. 2010, 363 (17): 1631-1637.