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Product Name:

Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Product Type : ELISA Kit
Code : CSB-E16539m
Size : 96T,5×96T,10×96T
Uniprot NO. : Q8QZR5
Abbreviation : ALT/GPT
Alias : AAT1, ALT1, GPT1, glutamic pyruvate transaminase|glutamic-alanine transaminase 1
Species : Mouse
Sample Types : serum, plasma, cell culture supernates, cell lysates
Detect Range : 0.156 mU/ml-10 mU/ml
Sensitivity : 0.039 mU/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %83-95
1:2Average %97
Range %92-101
1:4Average %106
Range %98-113
1:8Average %94
Range %87-97
Recovery :
The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-97
EDTA plasma (n=4)10295-108
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
102.645 2.566 2.606 2.421
52.015 1.855 1.935 1.750
2.51.194 1.155 1.175 0.990
1.250.777 0.796 0.787 0.602
0.6250.492 0.461 0.477 0.292
0.3120.335 0.344 0.340 0.155
0.1560.278 0.288 0.283 0.098
00.191 0.178 0.185
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