Custom Polyclonal Antibody Production

ELISA
Antigen coating concentration 2 ug/ml
Antiserum 1:2000 is more than diluted
Secondary：Goat polyclonal to rabbit IgG at 1/50000 dilution

Western Blot
Positive WB detected in：K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate,Rat liver tissue, Mouse brain tissue, Mouse lung tissue
All lanes：a antibody at 2ug/ ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 KDa
Observed band size: 64 KDa

Western blot
WT: Wild-type 293 cells
KO: Knockout 293 cells

IHC image of A antibody diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with A antibody at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).