Your Good Partner in Biology Research
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Your Good Partner in Biology Research
Exosomes are small membrane vesicles (30–150 nm) of endocytic origin, which are shed by all cell types under normal- and patho-physiological conditions. Exosomes have pleiotropic physiological and pathological functions and play significant roles in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes have received significant attention for their roles in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation such as differential ultracentrifugation, density gradient ultracentrifugation, ultracentrifugation, chromatography, magnetic bead immunoassay, polypolymer precipitation method, etc. However, the quick and easy operation of exosome isolation kits is irreplaceable.
CUSABIO Exosome Isolation Kits purify exosome by affinity purification. High Purity exosomes and other Evs (Extracellular vesicles) from cell culture medium and body fluid (high yield by normal microfiltration) can be easily obtained by this method.
The experiments include Transmission electron microscope analysis, Nanoparticle tracking analysis, NanoFCM analysis, Western Blot, Fluorescence quantitative (qPCR), High-Throughput Sequencing, etc.
CUSABIO Exosome Isolation Kits are available to isolate exosomes from the supernatant of various cells, such as A375, HEPG2, PC-3, Hela, U87, MG63, etc.
ENO1 (CSB-MA007670A0m)
GAPDH (CSB-MA000071M0m)
ENO1 (CSB-MA007670A0m)
GAPDH (CSB-MA000071M0m)
ENO1 (CSB-MA007670A0m)
GAPDH (CSB-MA000071M0m)
We did some contrast experiments to compare the exosomes extracted by kits of CUSABIO and other companies. It turns out that CUSABIO Exosome Isolation Kits have better performance.
The exosomes extracted by CUSABIO kits are complete in shape, spherical or dish-shaped, with uniform particles. Vesicles are structurally intact. The following are a part of the results.
CUSABIO (CSB-EI0102, CSB-EI0110)
Company A
There are up to 3 x 1011 particles of purified exosomes per millilitre.
CUSABIO (CSB-EI0102, CSB-EI0110)
Particle size: 79.19 nm
Concentration: 3.08*1011 particles /mL
Company A
Particle size: 56.03 nm
Concentration: 9.62*108 particles /mL
The exosomes extracted by CUSABIO Exosome Isolation Kit are superior to the other three well-known brand kits and comparable to ultracentrifugation.
No ultracentrifugation or phenol/chloroform step required. No equipment requirement. Just follow our protocol, you will get the exosomes soon.
Prices of CUSABIO exosome isolation kits are very competitive. We have an efficient production and management system so that we can manufacture premium reagents at less cost and our customers can get better quotes.
What's more, CUSABIO has a powerful technical support team and complete after-sales service here to meet the needs of every purchase so that you have no worry and save time & energy for your research.
| Code | Size | Sample Types | Instructions |
|---|---|---|---|
| CSB-EI0102 | 2T | Cell culture supernates | Download |
| CSB-EI0110 | 10T | Cell culture supernates | Download |
Store at 4℃ for short term storage or -80℃ for long term storage. It is recommended to store it after aliquoting to avoid repeated freezing and thawing.
a. Ultra-centrifuge the cell culture serum for 10 hours to remove exosomes from the serum (aseptic operation).
b. Choose serum-free media for cell culture.
c. Choose exosome-free serum for cell culture.
WB validation of specific markers, transmission electron microscope (TEM), particle size (NanoFCM, NTA), etc.
a. Simple, stable and highly repeatable.
b. No equipment requirement.
Any questions, please feel free to send emails to cusabio@cusabio.com.
Why Study Exosomes?
Extracellular vesicles (EVs) are small membrane vesicles, composed of a lipid bilayer with inserted transmembrane proteins, enclosing cytosolic components (protein, mRNA, and miRNA) derived from the EV-producing cells.
These EVs such as exosomes have been found in biofluids, including blood, saliva, urine, cerebrospinal fluid (CSF) and latex, act as messengers of intercellular communication networks. EVs carry lipids, proteins, and RNAs derived from their producing cells, and have potential as biomarkers specific to cell types and even cell states.
You can click here to know more about Exosomes.
How Do You Get Exosomes?
However, conventional methods (such as ultracentrifugation or polymeric precipitation) for isolating EVs have disadvantages regarding purity and feasibility. You can click here to get the comparision of five exosome isolation methods.
An exosome isolation kit can solve all your problems on exosome isolation. It is also the reason why CUSABIO keeps invest in developing high quality exosome isolation kits for so many years.
