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A high-efficient exosome isolation kit can help a lot for every exosome researchers. High purity, high yield, and easy operation make it a great research tool.
As we know, exosomes are small membrane vesicles (30–150 nm) of endocytic origin, which are shed by all cell types under normal- and patho-physiological conditions. Exosomes have pleiotropic physiological and pathological functions and play significant roles in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes have received significant attention for their roles in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation such as differential ultracentrifugation, density gradient ultracentrifugation, ultracentrifugation, chromatography, magnetic bead immunoassay, polypolymer precipitation method, etc. However, the quick and easy operation of exosome isolation kits is irreplaceable.
CUSABIO Exosome Isolation Kits purify exosome by affinity purification. High Purity exosomes and other Evs (Extracellular vesicles) from cell culture medium and body fluid (high yield by normal microfiltration) can be easily obtained by this method.
The experiments include Transmission electron microscope analysis, Nanoparticle tracking analysis, NanoFCM analysis, Western Blot, Fluorescence quantitative (qPCR), High-Throughput Sequencing, etc.
CUSABIO Exosome isolation kit provides a simple and reliable method to extract intact exosomes from the cell culture supernatant, serum, plasma, Breast Milk, urine, saliva, Yeast, Plant.
CD9 (CSB-MA004969A0m )
ENO1 (CSB-MA007670A0m )
Exosomes extracted from Hela cells
Exosomes extracted from serum
Exosomes extracted from plasma
Exosomes extracted from Breast Milk
Exosomes extracted from saliva
Exosomes extracted from urine
Exosomes extracted from pichia pastoris
Exosomes extracted from kluyveromyces lactis
CUSABIO Exosome Isolation Kits are available to isolate exosomes from the supernatant of various cells, such as A375, HEPG2, PC-3, Hela, U87, MG63, etc.
ENO1 (CSB-MA007670A0m)
GAPDH (CSB-MA000071M0m)
ENO1 (CSB-MA007670A0m)
GAPDH (CSB-MA000071M0m)
ENO1 (CSB-MA007670A0m)
GAPDH (CSB-MA000071M0m)
CD63 (CSB-MA004950A0m)
CD63 (CSB-MA004950A1m)
The exosomes extracted by CUSABIO kits are complete in shape, spherical or dish-shaped, with uniform particles. Vesicles are structurally intact. The following are a part of the results.
There are up to 3 x 1011 particles of purified exosomes per millilitre.
CUSABIO (CSB-EI0102, CSB-EI0110)
Particle size: 79.19 nm
Concentration: 3.08*1011 particles /mL
The exosomes extracted by CUSABIO Exosome Isolation Kit are superior to the other three well-known brand kits and comparable to ultracentrifugation.
No ultracentrifugation or phenol/chloroform step required. No equipment requirement. Just follow our protocol, you will get the exosomes soon.
Prices of CUSABIO exosome isolation kits are very competitive. We have an efficient production and management system so that we can manufacture premium reagents at less cost and our customers can get better quotes.
What's more, CUSABIO has a powerful technical support team and complete after-sales service here to meet the needs of every purchase so that you have no worry and save time & energy for your research.
| Code | Size | Sample Types | Instructions |
|---|---|---|---|
| CSB-EI0102 | 2T | Cell Culture Supernatant, Serum, Plasma, Breast Milk, Urine, Saliva | Download |
| CSB-EI0110 | 10T | Cell Culture Supernatant, Serum, Plasma, Breast Milk, Urine, Saliva | Download |
Store at 4℃ for short term storage or -80℃ for long term storage. It is recommended to store it after aliquoting to avoid repeated freezing and thawing.
a. Ultra-centrifuge the cell culture serum for 10 hours to remove exosomes from the serum (aseptic operation).
b. Choose serum-free media for cell culture.
c. Choose exosome-free serum for cell culture.
WB validation of specific markers, transmission electron microscope (TEM), particle size (NanoFCM, NTA), etc.
a. Simple, stable and highly repeatable.
b. No equipment requirement.
Exosomes contain cell-specific cargos of nucleic acids, proteins, lipids, and metabolites, which facilitate intercellular communication that contributes to a spectrum of biological processes in health and disease. Exosomes are related to immune responses, viral pathogenicity, chronic inflammation, neurodegenerative and cardiovascular diseases, even cancer progression. Additionally, exosomes are engineered to therapeutic nanocarriers for drug and gene delivery to the desired target, thus achieving the therapeutic control of many diseases.
Upon exosome binding to target cells through ligand-receptor interactions, the contents of exosomes are internalized into recipient cells. These biological active molecules, such as proteins, lipids, and genetic materials, that mediate exosomal intercellular communication, ultimately reprograming the recipient cells.
Exosomes play a dual role in human health. They keep cell health by removing damaged and unwanted proteins and structures in the cells. And they are also involved in immunity and reproduction in healthy individuals. However, cancer-derived exosomes participate in tumor growth, progression, dissemination, angiogenesis, and metastasis by transferring their cargoes of cytokines, mRNAs, and miRNAs to targeted cells. Anti-cancer therapy resistance is also attributed to exosomes of tumor origin. Besides, some exosomes are implicated in the pathogenesis of the disease, including neurodegenerative and cardiovascular diseases.
Exosome therapy is a form of regenerating treatment in which therapeutic payloads are delivered through exosomes to the affected site to achieve therapeutic effects. Therapeutic exosomes are administered through injection or infusion into the treatment location, where they target and release their content of endogenous and modified bioactive molecules, such as miRNA and proteins into damaged cells or tissues, stimulating quicker healing and diminish inflammation.
Compared to stem cells, exosomes are simpler, safer, cheaper, more easily stored and transported. In terms of therapeutic effects, exosome shows very low immunogenicity and do not trigger complications. Additionally, exosomes can not replicate, do not deteriorate, and are not infected by viruses.
Why Study Exosomes?
Extracellular vesicles (EVs) are small membrane vesicles, composed of a lipid bilayer with inserted transmembrane proteins, enclosing cytosolic components (protein, mRNA, and miRNA) derived from the EV-producing cells.
These EVs such as exosomes have been found in biofluids, including blood, saliva, urine, cerebrospinal fluid (CSF) and Breast Milk, act as messengers of intercellular communication networks. EVs carry lipids, proteins, and RNAs derived from their producing cells, and have potential as biomarkers specific to cell types and even cell states.
You can click here to know more about Exosomes.
How Do You Get Exosomes?
However, conventional methods (such as ultracentrifugation or polymeric precipitation) for isolating EVs have disadvantages regarding purity and feasibility. You can click here to get the comparision of five exosome isolation methods.
An exosome isolation kit can solve all your problems on exosome isolation. It is also the reason why CUSABIO keeps invest in developing high quality exosome isolation kits for so many years.
