Over more than 18 years, CUSABIO Protein Expression Platform has established five recombinant expression systems, which includes Escherichia coli (E.coli) expression system, Pichia pastoris (Yeast) expression system, Baculovirus-infected insect cells expression system, Mammalian cells expression system and vitro E.coli expression system.
Vitro E.coli expression system, also known as the vitro translation system, simulates the life phenomena of biological cells and reproduces the transcription and translation process of intracellular proteins. Common components of a cell-free production include a cell extract, an energy source, a supply of amino acids, cofactors such as magnesium, and the DNA/mRNA with the desired genes which is the template of protein synthesis. The vitro protein synthesis of the target protein can be achieved by artificially controlling the substrates required for protein synthesis and transcription and translation-related protein factors.
CUSABIO's cell-free expression platform can provide you with complete technical services. It can solve specific problems related to protein expression, such as low protein yield, expression of special proteins (such as membrane proteins, toxic proteins, etc.), protein complexes production, parallel synthesis of many different proteins.
There are some features of vitro E.coli expression system as follows:
A. Compared with the traditional protein expression system, many processes are omitted, such as transformation of plasmids, cell culture, collection, crushing and centrifugation etc., which greatly improves the working efficiency;
B. The reaction system is small and can simultaneously parallel synthesizing many different proteins;
C. Short reaction cycles, meeting the scientific requirements for high-throughput ligand screening and proteomics;
D. The open reaction system is more easy change the reaction conditions and is conducive to the regulation of gene transcription, protein synthesis and post-translational modifications, and avoids the formation of inclusion bodies;
E. Stable reaction system, can be coupled with other processes to form automated, procedural, and scale production and accelerate the purification, functional characterization and subsequent structural analysis of recombinant proteins;
F. No cell structures restriction, can be used to produce exogenous proteins that are toxic to the host as it seeks to avoid the lethal effect of protein expression on host cells;
G. Add unnatural amino acids or isotopically labeled amino acids to express specific proteins.
H. Significant improvements have been made in items that have difficulty to express in common cell lines due to multiple transmembrane or hydrophobic conditions.
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Pichia pastoris (Yeast) expression system | Advantages |
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Baculovirus-infected insect cells expression system | Advantages |
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Mammalian cells expression system | Advantages |
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vitro E.coil expression system | Advantages |
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Expression System | System Benefits | Application | Features of CUSABIO |
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In vitro E.coli Expression System | Simple, take short time, high expression quantity, open and flexible, easy to express specific proteins, prepare protein complexes, parallel to synthesize a variety of different proteins, etc. | Toxic proteins, membrane proteins | Small amount expression conditions fumble, solve relative problems professionally, greatly reduce the experimental period, increase the expression quantity |
E. coli Expression System | High targeted gene expression quantity, low cost, simple culture conditions, product rapidly, strong scalability, simple conversion operation, easy to form disulfide bond | Prokaryotic proteins, simple eukaryotic proteins | Expression includes soluble protein, inclusion body, fusion proteins, etc., with wealthy experience and expertise, we can solve a variety of bottlenecks during the protein expression process |
Yeast Expression System | Cost-effective, low-cost for amplifying medium, simple culture conditions, production rapidly, strong scalability, good choice for secretory protein or intracellular protein expression, secrete proteins efficiently and allow simple purification, extensive post-translational modifications, no endotoxin | Industrial strain improvement, amplification | The combination of self-transformed efficient secretion vector and host can achieve the highest quality protein expression to the maximum extent; Patented Biobrick technology can be successfully used to the improvement and optimization of industrial strain |
Insect baculovirus expression system | Large gene capacity, high efficiency of exogenous gene expression, effective cell fold, moderate scalability, extensive post-translational modifications, glycosylation similar to mammalian cells, is relatively easy enzymatic deglycosylation, no endotoxin | Virus vaccines, signal proteins, cytokines, kinases, etc. | Adopt AcNPV-sf9 cells and high5 cells two expression systems, the selectivity of multiple expression systems, multiple hosts, multi-carrier greatly improve the success rate of protein expression |
Mammalian Cell Expression System | Higher expression levels, moderate scalability, cell suspension culture characteristic can do mass production, effective protein fold, suitable for protein secretion, full post-translational modifications, no endotoxin | Complex higher eukaryotes proteins | Adopt the specific combined methods of mammalian cell expression vector and a variety of transfections, optimize expression conditions, improve transfection efficiency, greatly shorten the experimental period, significantly increase the expression quantity |
A. Question about Tag removal, WB identification for Tag antibody, Endotoxin removal, aseptic manufacture process
Answer: Generally, these services such as WB identification of Tag antibody, Endotoxin removal, aseptic manufacture process can apply to all proteins include proteins which are expressed with E.coli system, but the tag removal service depends on the specific proteins, some proteins can remove tag but some can't. The lead time will prolong 2-3 working days accordingly for these services.
B. Question about shipping formats
Answer: The default shipping formats are liquid forms if you don’t have special demands. However, if you have special requirement on the shipping formats, please remark clearly when placing the order.
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