Tag/Control Antibodies

CUSABIO Tag/Control Antibodies include highly specific monoclonal and polyclonal antibodies.

What is Tag Antibodies?

Tag antibody is a specific region or group of an antigen, which determines the specificity of an antigen. The tag antibody can be used to detect expression, intracellular localization, and purification, qualitative or quantitative detection of the tag fusion protein. Fusion tags can be divided into two broad categories based on their relative molecular mass: large protein molecules and small polypeptide fragments. Please kindly view Tag Antibodies, Your Good Partner in Protein Research for more information.

All CUSABIO tag antibodies are purified by antigen affinity, and they can specifically bind to the corresponding tag proteins, then identify and purify the target proteins as well. Some of our tag antibodies are listed as below for your reference.

What is Control Antibodies?

Control antibodies, as the name means, are series of internal control antibodies. Generally speaking, control antibody can be divided into two groups, including isotype control antibodies and loading control antibodies. CUSABIO offers a wide selection of monoclonal loading control antibodies, including GAPDH, α-tubulin, and β-actin for several species. We also have some conjugated control antibodies, such as HRP- or biotin- conjugated control antibodies. Please kindly view the article How to Choose the Loading Control Antibodies for more information.

Some of our control antibodies are listed as below for your reference.

The Features of CUSABIO Tag/Control Antibodies

• The diversity of Tags and Controls
• High Sensitivity: Detects Lower Expression Levels

Western Blot analysis of HA-Tag Monoclonal Antibody (CSB-MA000141M0m)

Western Blot analysis of E-Tag Monoclonal Antibody (CSB-MA000151M0m)

Western Blot analysis of GAPDH Monoclonal Antibody (CSB-MA000071M0m)

• Higher reliability: Small difference between batches

TUBA1A Monoclonal Antibody (CSB-MA754656A0m)

Lot Number: I0308A

Lot Number: I0424A

Flag Tag Monoclonal Antibody (CSB-MA000021M0m)

Lot Number: I0603A

Lot Number: I0419A

• Multiple Validations

HA-Tag Monoclonal Antibody (CSB-MA000141M0m)

Western Blot (WB)

Positive WB detected in: 3 different overexpression lysates with HA tagged
All lanes: HA-Tag antibody at 1:1000

Flow cytometry (FC)

Overlay histogram showing 293F transfected cells stained with CSB-MA000141M0m (red line) at 1:100.

Immunoprecipitation (IP)

Immunoprecipitating HA-Tag in 293F transfected whole cell lysate.
Lane 1: Mouse control IgG (1µg); Lane 2: CSB-MA000141M0m (5µg) + 293F transfected whole cell lysate (500µg); Lane 3: 293F transfected whole cell lysate (20µg)

Immunofluorescence (IF)

Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI.

GAPDH Monoclonal Antibody (CSB-MA000071M0m)

Western Blot (WB)

Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: GAPDH antibody at 1:2000

Immunoprecipitation (IP)

Immunoprecipitating GAPDH in Hela whole cell lysate. Lane 1: Mouse control IgG; Lane 2: CSB-MA000071M0m (3μl) + Hela whole cell lysate (500μg); Lane 3: Hela whole cell lysate (20μg)

Flow cytometry (FC)

Overlay histogram showing Hela cells stained with CSB-MA000141M0m (red line) at 1:200.

Immunofluorescence (IF)

Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI.

Immunohistochemistry (IHC)

IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.