ELISA kits are a quick, convenient, and accurate research tool for the detection and quantitation of targets of interest in cultures and samples. Because of their exceptional advantage, ELISA kits are used in many important research areas, including cancer, cardiovascular, cell biology, epigenetics, metabolism, immunology, neuroscience, signal transduction, etc.
CUSABIO has 13+ years of experience in manufacturing ELISA kits and has formed a sound platform for the development of ELISA kits.
As a trustworthy ELISA kit supplier, we offer 4000+ ELISA test kits mainly in sandwich ELISA format, including 3000 targets, covering species of human, mouse, rat, bovine, etc.
As your partner in biological research, we completely understand the hardships of your work, and we try our best support to your research by optimizing every detail of our products and service.
CUSABIO has the strictest quality control system to ensure every ELISA kit has no quality issues so that you can get accurate data in your experiments. And CUSABIO technical team will be on your back all the time.
Each ELISA kit consists of a coated plate and a ready-to-use solution. No special handling required. Simple operation.
- Multiple Species
CUSABIO ELISA kits cover more than 30 species with broad research areas, including human, mouse, rat, bovine, rabbit, pig, sheep, goat, monkey, etc.
- High Sensitivity
We randomly compared our ELISA kits sensitivity with other suppliers'. Click here to read the data.
- Multiple Biological Samples
CUSABIO ELISA kits are validated in multiple biological samples:
serum, plasma, body fluids, such as urine, cell culture supernatant, cell/ tissue lysate, tear, breast milk, saliva, etc.
- Cited in more than 7000 References
CUSABIO ELSA kits are cited in more and more publications in well-known magazines such as Nature, PLOS One, JASN, Bio-Factors, Scientific Reports, APS and so on.
ELISA Kits Catalog
Here is the catalog of all ELISA kits. You can find the right ones for your research, or search them in the search box on the header part.
What is ELISA?
ELISA, short for enzyme-linked immunosorbent assay, is a method to detect the presence of a ligand, which usually is a protein, in a liquid sample using antibodies directed against the protein to be measured.
Compared with other immunoassay methods, ELISA is featured with higher sensitivity, specificity and throughput. Moreover, it is easy to perform. These advantages make ELISA be a very popular choice for researchers from various areas.
How does an ELISA Kit Work? What is the Typical ELISA Workflow?
Firstly, the antigens of interest are immobilized on the microplate by overnight incubation. This step forms antigen-coated wells.
Secondly, add the enzyme-labeled antibodies to the wells. Sometimes the unconjugated antibodies are added before the addition of the enzyme-labeled antibodies according to the experiment requirement. The antigens are directly captured by the enzyme-labeled primary antibodies or indirectly captured via the enzyme-labeled secondary antibodies that have been attached to the unconjugated antibodies on the microplate.
Thirdly, specific biotinylated detection antibodies are added to the wells to enable detection of the captured antibodies. The addition of the substrate solution is then added to each well. The color reaction is presented after incubation of the plated at 37 degrees celsius for 5 minutes.
Here is the typical ELISA workflow.
Click here to read a detailed ELISA Protocol, which includes Direct ELISA Protocol, Indirect ELISA Protocol, and Sandwich ELISA Protocol.
Select Right ELISA kits for Your Experiments
Once mentioned ELISA, the first problem is how to choose a right ELISA kit for experiments. Before that, you must distinguish the four common types of ELISA, including direct ELISA, indirect ELISA, sandwich ELISA and competitive ELISA, and figure out the advantages and disadvantages of each ELISA.
Start your ELISA Experiment
After choosing your right ELISA kit, a guide on how to do the pre-experiment before ELISA experiment must be required. And we have prepared it for you. Besides a general ELISA Protocol is also ready.
In addition, the CUSABIO's technicians have summarized the process of how to do ELISA data analysis after accomplished the experiment. We believe it is very helpful.
If you get into trouble in the process, don't forget to check the common problems of ELISA experiment and ELISA FAQs. You may find answers to the problems.
Rat Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit referenced in "Experience on the diagnosis and treatment of multiple active primary cancers in our Institution". Journal of Clinical Oncology, 2021
Human Undercarboxylated Osteocalcin (ucOC) ELISA Kit referenced in "Increased lipocalin 2 levels in adolescents with type 2 diabetes mellitus". Journal of Pediatric Endocrinology and Metabolism,2021
Human complient factor H,CFH ELISA Kit referenced in "Relationship between advanced glycated end products and maternal cognition in gestational diabetes: a case control study". The Journal of Maternal-Fetal & Neonatal Medicine, 2021
Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit referenced in "MiR-133a is a potential target for arterial calcification in patients with end-stage renal disease". International Urology and Nephrology, 2021
Rat prostate specific antigen,PSA ELISA Kit referenced in "Standardized Saw Palmetto Extract Directly and Indirectly Affects Testosterone Biosynthesis and Spermatogenesis". Journal of Medicinal Food, 2021
Read More >>
Request Trial Samples
24T trial size is available for all ELISA kits except food safety ELISA kits.
Only one 24T trial size kit can be applied for each product for a single customer. No quantity limit for products you can apply.
How to apply for 24T trial size:
StepⅠ: Search for the ELISA kit you need on our website.
StepⅡ: Apply for 24T trial size at the product page.
Explore More >>