Product Features

● 8 Key Quality Control Standards

CUSABIO ELISA kits are ready-to-use ELISA kits with complete reagents, strict quality control, and validated performance. During the development process, multiple stringent steps are taken to ensure the outstanding quality of the kits, guaranteeing the products provided to customers have high standards in terms of linearity, specificity, stability, and sensitivity.

01
Detection Range

The actual detection sample value is calculated through the standard curve, and the correlation coefficient R of the standard curve for quantitative kits is greater than 0.99.

Take CSB-E07270h (Human Adiponectin (ADP) ELISA Kit) as an example of the standard curve.

ng/ml OD1 OD2 Average Corrected
100 2.839 2.897 2.868 2.718
50 1.968 1.974 1.971 1.821
25 1.144 1.192 1.168 1.018
12.5 0.709 0.733 0.721 0.571
6.25 0.433 0.450 0.441 0.291
3.125 0.303 0.301 0.302 0.152
1.562 0.242 0.223 0.233 0.083
0 0.149 0.151 0.150
CSB-E07270h standard curve
02
Specificity

Specificity is a key indicator of ELISA kits. It is mainly affected by the interference of highly homologous proteins between different species, target protein ligands, or target protein receptors.

CUSABIO selects raw materials with high specificity to ensure the excellent specificity of ELISA kits, analyze cross-reactions and interference, and confirm that only the target analyte can be identified and detected.

03
Lower Detection Limit

The lower detection limit is an indicator of sensitivity. It is determined by factors such as the antibody titer used, and during the development process, the sensitivity and low background detection results are generally optimized to significantly improve the sensitivity of the kit. The lower the detection limit, the higher the sensitivity.

Sensitivity is determined by adding 3 standard deviations to the average O.D. obtained by repeating the zero standard.

04
Linearity

Linearity is a criterion for determining the accuracy and specificity of the kit. To evaluate the linearity of the assay, samples with high concentration analytes are added to various matrices and diluted with sample diluent to produce samples with values within the dynamic range of the assay. Dilution linearity tests are performed for each validated sample type; if the results are within 80%-120% of the expected concentration for each dilution, the linearity is considered good. Linearity is crucial for the accurate measurement of analyte concentrations within the detection dynamic range.

Taking CSB-E07270h (Human Adiponectin (ADP) ELISA Kit) for example: Serum samples are diluted at 1:100, 1:200, 1:400, and 1:800. Cell culture supernatants are diluted at 1:2, 1:4, 1:8, and 1:16. The test values show good linearity within 80-120%.

Sample Serum(n=4)
1:100 Average( %) 91
Range ( %) 85-97
1:200 Average( %) 97
Range ( %) 91-103
1:400 Average( %) 93
Range ( %) 85-95
1:800 Average( %) 95
Range ( %) 90-100
Sample Cell Culture Supernates(n=4)
1:1 Average( %) 95
Range ( %) 83-101
1:2 Average( %) 97
Range ( %) 86-115
1:4 Average( %) 90
Range ( %) 83-98
1:8 Average( %) 91
Range ( %) 80-107
05
Recovery Rate

Recovery rate = measured target protein concentration / added target protein concentration * 100%

It reflects the degree of analyte loss during the sample analysis process; the higher the recovery rate, the higher the accuracy.

Recovery experiments can test whether the kit is affected by interfering factors. Using a natural sample matrix without added protein as a blank control, the recovery rate should be between 80% and 120%, indicating good kit performance.

For example, the recovery rate of CSB-E07270h (Human Adiponectin, ADP ELISA Kit):

Sample Type Average(%) Range(%)
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
06
Accuracy

The coefficient of variation (CV%) represents the consistency of the measurement, i.e., the degree of conformity for repeated measurements of the same sample.

Intra-assay accuracy: The precision testing method within the same batch: measure low, medium, and high-value test samples with the same batch of products 20 times (n) each, calculate the average and standard deviation (SD) of the 20 measurement results, and calculate the coefficient of variation (CV) using the formula CV = standard deviation/average × 100%.

To evaluate the accuracy of CSB-E07270h (Human Adiponectin (ADP) ELISA Kit): CV% <8%.

Inter-assay accuracy: The precision testing method between different batches: measure low, medium, and high-value test samples with three batches of products 20 times (n) each, calculate the average and standard deviation (SD) of the 20 measurement results, and calculate the coefficient of variation (CV) using the formula CV = standard deviation/average × 100%.

To evaluate the accuracy of CSB-E07270h (Human Adiponectin (ADP) ELISA Kit): CV% <10%.

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(ng/ml) 5.345 16.273 29.471 29.471 20.841 34.563
SD 0.24 0.62 0.62 0.43 1.65 2.34
CV(%) 4.49 3.81 6.42 5.14 7.92 6.77
07
Stability

The better the stability, the longer the storage time.

The main components and assay plates of CUSABIO ELISA kits are subjected to heat damage testing at 37°C for 7 days, and then compared with materials stored at 4°C. After 7 days, if the OD value is ≤30%, this is considered satisfactory.

Some reagents can withstand high-temperature heat damage at 60°C, which greatly minimizes the inactivation of antigens and antibodies during long-distance transportation and storage.

08
Natural Sample Validation

CUSABIO ELISA kits can detect natural samples, selecting appropriate natural samples (serum, plasma, cell supernatant, etc.) according to the target properties, providing customers with the most accurate experimental data reference.

Taking CSB-E07270h (Human Adiponectin (ADP) ELISA Kit) as an example, we previously tested serum and plasma samples at a 1:500 dilution, and the test values were S3-S5.

● 15 Research Areas

● 20+ Species

CUSABIO ELISA kits cover more than 20 species, involving multiple research areas, meeting the needs of different customers.

● Wide Range of Detectable Samples

CUSABIO ELISA kits can detect various biological samples, including serum, plasma, body fluids such as urine, cell culture supernatants, and cell/tissue lysates.

● Ready for Research Use

Each ELISA kit contains a plate coated with antibodies and ready-to-use reagents. Simple operation, no special handling is required.

● 20,000+ Published Publication

CUSABIO ELISA kits are trusted by thousands of researchers worldwide and have been cited in many prestigious high-impact journals, such as Nature, Science, Cell, Immunity, Nature Medicine, Cell Metabolism, Gastroenterology, etc.


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Customer Reviews

Write Product Reviews
Average Rating:
4.9 - 205 reviews

Review: MUC5AC levels in bronchoalveolar lavage fluid (BALF) of normal and smoking C57B/L mice were detected by CSB-E11040m. The OD values of normal mice ranged from 0.061 to 0.415, and those of smoking mice ranged from 0.652 to 0.776. Simple and convenient operation, recommended!

Review: We used CSB-E04639m to measure the IL-6 level in bronchoalveolar lavage fluid of normal and smoking C57B/L mice. The OD value of normal group was 0.049-0.478, and that of smoking group was 0.844-1.252.

Review: CSB-E08054m was used to detect the levels of IL-1β in bronchoalveolar lavage fluid of normal and smoking C57B/L mice. The OD values of the standard curve were 1.717, 1.113, 0.715, 0.443, 0.257, 0.204, 0.145, 0.074. The OD values of normal mice ranged from 0.055 to 0.404, and the OD values of smoking mice ranged from 0.533 to 1.011. Simple and convenient operation, recommended!

Review: CSB-E14068m kit was used to detect the serum level of CRF in mice. After 5 times of dilution, the normal and model groups and the administration groups were all within the range of standard curve. All experiments were completed within three and a half hours, and the test results were stable and the standard curve was good

Review: CSB-E14068m kit was used to detect the serum level of CRF in mice. After 5 times of dilution, the normal and model groups and the administration groups were all within the range of standard curve. All experiments were completed within three and a half hours, and the test results were stable and the standard curve was good

Review: CSB-E06874m kit was used to detect the serum ACTH level of mice. After 4 times dilution, the serum ACTH level of the normal group, the model group and the administration group were mostly within the standard curve range, but if the sample was sufficient, 1:1 dilution or the use of stock solution could be considered, and the stock solution was still within the standard curve range after the conversion of dilution. All experiments were completed within 6 hours. The results were stable and the standard curves were good.

Citations

Mouse Lipopolysaccharides(LPS) ELISA Kit cited in "Hepatic pIgR-mediated secretion of IgA limits bacterial translocation and prevents ethanol-induced liver disease in mice". Gut, 2023

Fish adrenocorticotropic hormone (ACTH) ELISA kit cited in "Acute stress response on Atlantic salmon: a time-course study of the effects on plasma metabolites, mucus cortisol levels, and head kidney transcriptome profile". Fish physiology and biochemistry, 2023

Human Interleukin 6,IL-6 ELISA KIT cited in "Novel Siprulina platensis Bilosomes for Combating UVB Induced Skin Damage". Pharmaceuticals, 2023

Human irisin ELISA kit cited in "Relationship among Body Composition, Adipocytokines, and Irisin on Exercise Capacity and Quality of Life in COPD: A Pilot Study". Biomolecules, 2023

Rat cyclooxygenase-2,COX-2 ELISA Kit cited in "Cyclooxygenase-2 activates EGFR–ERK1/2 pathway via PGE2-mediated ADAM-17 signaling in testosterone-induced benign prostatic hyperplasia". Inflammopharmacology, 2023

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Request Trial Samples

24T trial size is available for all ELISA kits except food safety ELISA kits.

Only one 24T trial size kit can be applied for each product for a single customer. No quantity limit for products you can apply.

How to apply for 24T trial size:

StepⅠ: Search for the ELISA kit you need on our website.

StepⅡ: Apply for 24T trial size at the product page.

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