Custom Recombinant Antibody Production

Recombinant antibody, also known as genetic engineering antibody, refers to the usage of recombinant DNA and protein engineering technology to modify or recombine antibody genes according to various needs, then expressed after transfection via appropriate receptor cell.
Cusabio could offer one-stop service of recombinant antibody production to customers upon your various needs.
The production process of Cusabio custom recombinant antibody is as follows.

Process & Deliverables:

Antigen Preparation Process Deliverables Production Time
Peptide Synthesis
  • Select Positive Gene
  • Recombinant Antibody Expression
  • Protein A/G Purification
  • ELISA, WB Validation
  1. QC report of antigen;
  2. 100ug×purified recombinant antibodies together with QC report, including the information of gene sequencing, concentration, purity and endotoxin content;
  3. WB positive guarantee for antigen;
  4. Other applications(IHC, IF, IP, FC, ChIP) could be available upon customers’ requests.
8-16 weeks
Recombinant Protein Production 12-16 weeks
Native Protein Selection 12-16 weeks

Successful Showcase (partial)

Western Blot
Positive WB detected in:A549 whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysate
primary antibody: Phospho-RSK1 antibody at 1.75µg/ml
Secondary antibody:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 90 KDa
Observed band size: 90 KDa

Western Blot
Positive WB detected in:Hela whole cell lysate, A549 whole cell lysate,293 whole cell lysate
primary antibody:Phospho-POLR2A antibody at 1.02µg/ml
Secondary antibody:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 270 KDa
Observed band size: 270 KDa

Western Blot
Positive WB detected in:Jurkat whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysate(treated with EGF or Pervanadate)
primary antibody:Phospho-LAT antibody at 2.9µg/ml
Secondary antibody:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 KDa
Observed band size: 38 KDa

Western Blot
Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not)
primary antibody: Phospho-SHP2 antibody at 0.65µg/ml
Secondary antibody:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 KDa
Observed band size: 68 KDa

Quote

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ANTIBODY QUOTE #1

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