Recombinant antibody, also known as genetic engineering antibody, refers to the usage of recombinant DNA and protein engineering technology to modify or recombine antibody genes according to various needs, then expressed after transfection via appropriate receptor cell.
Cusabio could offer one-stop service of recombinant antibody production to customers upon your various needs.
The production process of Cusabio custom recombinant antibody is as follows.
Process & Deliverables:
Antigen Preparation | Process | Deliverables | Production Time |
---|---|---|---|
Peptide Synthesis |
|
|
8-16 weeks |
Recombinant Protein Production | 12-16 weeks | ||
Native Protein Selection | 12-16 weeks |
Successful Showcase (partial)
Western Blot
Positive WB detected in:A549 whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysate
primary antibody: Phospho-RSK1 antibody at 1.75µg/ml
Secondary antibody:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 90 KDa
Observed band size: 90 KDa
Western Blot
Positive WB detected in:Hela whole cell lysate, A549 whole cell lysate,293 whole cell lysate
primary antibody:Phospho-POLR2A antibody at 1.02µg/ml
Secondary antibody:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 270 KDa
Observed band size: 270 KDa
Western Blot
Positive WB detected in:Jurkat whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysate(treated with EGF or Pervanadate)
primary antibody:Phospho-LAT antibody at 2.9µg/ml
Secondary antibody:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 KDa
Observed band size: 38 KDa
Western Blot
Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not)
primary antibody: Phospho-SHP2 antibody at 0.65µg/ml
Secondary antibody:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 KDa
Observed band size: 68 KDa
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