Transmembrane Protein Expression Service


Transmembrane Proteins Overview

Among the numerous genes in the genome, approximately 20% to 30% encode membrane proteins. These membrane proteins participate in almost every cellular activity, including ligand-receptor binding, signal transduction, molecular transport, cell recognition, and enzyme catalysis, rendering them of great significance in biological research and drug development. Therefore, membrane proteins represent crucial targets for numerous drug development and clinical therapeutic studies, with ion channels, transporters, GPCRs, and kinases being the largest class of drug targets. Approximately 40% of approved antibody drugs currently exert their pharmacological effects by targeting GPCR complexes.

However, due to their complex structures and challenging expression, research on multi-pass transmembrane proteins has always been a formidable task. CUSABIO has established three major transmembrane expression technology platforms, providing multi-pass transmembrane protein expression services aimed at resolving the difficulties encountered by researchers in studying these proteins. We are dedicated to delivering high-quality recombinant transmembrane proteins to scientists, facilitating advancements in the field of biomedical research.


How to Choose a Transmembrane Protein Technology Platform?

Different membrane protein technology platforms have their unique advantages and disadvantages. The selection should be based on the specific protein expression needs and application scenarios. You can consult with technical personnel from CUSABIO for specific guidance. Below is a comparison of different platforms:

Technology Platforms VLPs Detergent Micelle Platform Nanodisc Platform
Coating for ELISA Direct coating Direct coating Direct coating
Activity Great Good Good
Stability Most stable Relatively stable Relatively stable
Purity Mixture Relatively pure (only target protein) Mixture
SPR/BLI Detectable Detectable (solution adjustment required) Detectable
Factors to consider Total protein concentration; More costly. Relatively short preparation period, More affordable. Relatively short preparation period.

Why choose CUSABIO Transmembrane Protein Expression Service?

  • Efficient Expression Strategies: CUSABIO adopts three major transmembrane protein expression platforms, coupled with optimized vectors, to ensure efficient expression of multi-pass transmembrane proteins.
  • Rich Experience in Transmembrane Protein Expression: CUSABIO has over ten years of experience in transmembrane protein expression, with a highly experienced technical team having expressed over 360 full-length transmembrane proteins.
  • High-Quality Expressed Proteins: We implement strict quality control processes to provide expressed proteins with high purity and activity, suitable for further experimental research.
  • High Yield: The yield of cell-free expression system can reach up to 5mg/mL, and the yield of mammalian cell expression system can reach up to 100mg/mL.
  • Guarantee:
    CUSABIO Protein service Guarantee
  • Risk-free: We do NOT charge if we cannot deliver the protein.

Note: This risk-free custom protein service is only suitable for proteins within 800aa. We will charge according to different steps if you need to express proteins of more than 800aa.


Service Process

Mammalian Cell Expression System & Virus-like Particle (VLPs) Platform for Transmembrane Protein Expression

30-41 business days
Plasmid construction
Plasmid construction
13-16 business days
Small scale expression
Small scale expression
7-10 business days
Scale up expression and purification
Scale up expression and purification
7-10 business days
Quality Control
Quality Control
3-5 business days

In vitro E.coli Expression System & Detergent Micelle/Nanodisc Platform for Transmembrane Protein Expression

24-38 business days
Plasmid construction and preparation
Plasmid construction and preparation
13-16 business days
Small-scale expression and optimization
Small-scale expression and optimization
3-7 business days
Target protein expression and purification
Target protein expression and purification
5-10 business days
Quality Control
Quality Control
3-5 business days

Case Studies

Click to view all transmembrane protein products from CUSABIO

● Virus Like Particles (VLPs) Platform

Recombinant Human Somatostatin receptor type 2(SSTR2)-VLPs (Active)

Function ELISA
Function ELISA
TEM
TEM
Western Blot-His
Western Blot-His
FACS Blocking
FACS Blocking 01
FACS Blocking
FACS Blocking 02

Recombinant Human Claudin-6(CLDN6)-VLPs (Active)

VLP-CLDN6 ELISA
VLP-CLDN6 ELISA
VLP-CLDN6-GFP ELISA
VLP-CLDN6-GFP ELIS
Western Blot-His
Western Blot-His
VLP-CLDN6 ELISA
VLP-CLDN6 ELISA
LC-MS/MS
LC-MS/MS

● Nanodisc Platform


Customer Reviews

Write Product Reviews
Average Rating:
5.0 - 6 reviews

Review: CUSABIO\'s protein products have excellent activity data and purity, and their protocols are comprehensive. The service is also very good. Our experiments have been very successful, and the antibodies we screened have good specificity and high sensitivity. We will continue to purchase from CUSABIO in the future!

Review: I used the CSB-MP001627RA product to develop a competitive ELISA method, and the results showed that the protein had good binding activity, stable properties, and excellent repeatability, and all parameter indicators met the standards. It is a repeat purchase, and I will continue to purchase this product.

Review: The protein has seven transmembrane domains, making it very difficult for recombinant expression. I have purchased both of CUSABIO\'s mammalian cell and cell-free expressed products, and they have performed well in terms of activity determination, which are sufficient to meet my future application demands. I may continue to purchase in large quantities in the future.

Review: I used the CSB-MP003996HU protein for an ELISA experiment, and the EC50 was measured to be 0.8830-2.771 ng/ml, indicating good protein activity. In the subsequent antibody screening, we employed flow cytometric analysis to determine whether this protein can bind to molecules expressed on the cell surface. The results showed that some antibodies were able to bind to this protein. The protein quality is quite good.

Review: I used the CSB-MP004900HU protein with an anti-CD22 rabbit monoclonal antibody for an ELISA experiment, and the EC50 was measured to be 0.8979-1.508 ng/ml, indicating good protein activity. CUSABIO has rich experience in protein expression, and protein products have good activity and stable properties, providing great convenience and assistance for our research!

Review: CCR8 is a multi-pass transmembrane protein, and expressing only its extracellular domain can disrupt its conformational epitopes, resulting in the loss of some antigenic epitopes. Your company has developed this full-length protein with good biological activity and immunogenicity. It will be very helpful for our company\'s subsequent development of antibody drugs.

CUSABIO Protein Expression Quotation Request Form

I.Your Information

  • Name*

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  • Email*

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  • Country*

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II.Product Information

  • Product Name (including species)*

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  • Uniprot No*

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  • Order Quantity

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  • Expression System

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  • Expression Region

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  • Sequence

    This sequence does not include the Tag sequence. Please fill if you have special demand.

  • Do you need tag ?

    Tag Removal Service

    If customer needs to remove the tag, please remark with tag removal service, otherwise, we won't remove the tag.

    There are extra charges for tag removal service. The cost will be list in the quotation.

    Not all protein tags can be removed as some proteins will be very unstable after tag removal.

    If we fail in removing the tag, we won’t charge for any extra cost, and remark this information in COA as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”

    Generally, the delivery time will be extended for 2-3 days.

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  • Molecular Weight

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III.Customer's Requirement(Click here to show more)

  • Tag Removal Service

  • Endotoxin Level Requirement

  • Aseptic Manufacture Processing

  • Western Blot Service

  • Purity Requirement

  • Concentration Requirement

  • Storage Buffer Requirement

  • Delivery Form Requirement

  • Package Size Requirement

  • Other Requirement

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