Custom Nanobody Service
Nanobodies, also known as VHH antibodies or single-domain
antibodies, are small antibody fragments derived from camelid species, primarily llamas and
camels. They are a type of antibody naturally occurring in these animals and are much smaller
than conventional antibodies found in humans (which have four protein chains).
The nanobodies screened from alpaca
libraries have many advantages, including small molecular weight, strong tissue
permeability, high stability, low immunogenicity and so on. CUSABIO has both alpaca
immunized library (VHH library) and alpaca naive library available, we can offer one-stop
solution from antigen production to qualified recombinant antibody to our customers. CUSABIO
nanobodies are favored by many customers, especially widely used for drug discovery.
Small molecular weight
Their small size (~15 kDa) allows them have strong tissue permeability, can
directly penetrate the blood-brain barrier, conducive to the diagnosis and targeted
therapy of tumors.
High stability
The high stability of the nanobody comes from the multiple disulfide bonds in
its interior, which can withstand the denaturating conditions such as high
temperature, strong acid and strong base, and can still be regenerated and regain
biological activity after treatment at 90℃.
Low immunogenicity
Nanobody does not contain Fc fragment, avoiding the immune reaction caused by Fc
fragment, and has a homology with human VH as high as 67%~87%, so nanobody also has
good biocompatibility with the biological samples of human species.
Short leading time of large scale production
Nanobody is hydrophilic and monopeptidyl, as well as lack of glycosylation
modification, so it can be produced in prokaryotic expression system with high cost
effectiveness. At the same time, nanobody is not easy to lose biological activity,
so it is easy for storage.
Alpaca Immune Molecule Screening
Alpaca Naive Library (30 business
days)
Service Process:
Requirements for antigens:
- 1mg, purity >90% (SDS-PAGE), concentration >10.5mg/mL and PBS formulation
(if not, please
inquire first).
Guaranteed deliverables
- Phage titer during the screening process
- Raw data from ELISA testing
- Sequencing and sequence analysis files of positive clones
- DNA sequences and amino acid sequences of no less than 10 positive
monoclonal clones
Guaranteed deliverables
- Antibody expression plasmid
- All affinity purified recombinant antibodies from 200 mL cell culture
supernatant will be
offered to customer, at least 100μg per one
- Antibody purity will be higher than 95%, tested by SDS-PAGE
Guaranteed deliverables
- Screened nanobodies can be guaranteed to be successfully validated through
ELISA with
antigen, and FACS with endogenous cell samples.
Note:
① Clients can provide us with an antigen or use our antigen production service.
② Requirements for client-supplied antigens: 1mg, purity >90% (SDS-PAGE), concentration
>10.5mg/mL and PBS
formulation (if not, please inquire first).
