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The construction of stable cell lines involves the introduction of exogenous genes into recipient cells, where they integrate into the cellular chromosome, enabling the sustained and stable expression of the target antigen on the host cell membrane. Stable cell lines play a pivotal role in biological research, widely employed in applications such as the production of recombinant proteins and monoclonal antibodies, drug screening, and gene function studies.
With years of expertise in protein expression research, CUSABIO possesses extensive experience in the development of stable cell lines. We are proficient in enhancing the production capacity of stable cell lines, thereby improving experimental outcomes. CUSABIO provides a comprehensive, one-stop service from gene sequencing to the delivery of stable overexpression cell lines and reporter gene cell lines, emphasizing our commitment to delivering high-quality solutions for our clients.
Overexpression stable cell lines refer to cell lines generated from a specific cellular lineage with the capability for sustained overexpression or interference of a particular gene. Their defining characteristic lies in their ability to continuously express specific genes, in contrast to transient transfection, which allows only transient expression.
Applications:
Reporter gene cell line construction involves the molecular biology cloning technique, where a specific reporter gene (e.g., luciferase) is inserted into the chromosome of the target cells. Through selection and cultivation processes, a cell line capable of stably expressing the reporter gene is obtained.
Applications:
Mature Stable Cell Line Development Platform
Equipped with comprehensive service platforms for cells, antibodies, proteins, etc., it can provide one-stop service from stable cell line construction to recombinant protein production.
Strict Quality Control System
Double detection of microorganisms such as bacteria and mycoplasma, ensuring 100% contamination-free, and cell viability testing is conducted to guarantee delivery quality.
Stable Cell Passage
High quality delivery standards ensure stable passage of cells for more than 20 generations.
Professional Technical Support
Professional technical personnel regularly report on project progress to ensure smooth progress of the project.
Our Commitment：No charges if it doesn't pass the flow cytometry detection.
Host Cell Detection (1-2 Weeks)
Lentiviral Expression Plasmid Construction (2-3 Weeks)
Lentivirus Packaging (1-2 Weeks)
Cell Pool Screening and Validation (1-2 Weeks)
Monoclonal Screening and Validation (2-3 Weeks)
Cell Line Quality Control (1-2 Weeks)
Host Cell Detection (1-2 Weeks)
Reporter Gene Expression Plasmid Construction (2-3 Weeks)
Transient Transfection of Cells (1-2 Weeks)
Cell Pool Screening and Functional Activity Validation (1-2 Weeks)
Monoclonal Screening and Validation (7-10 Weeks)
Cell Line Quality Control (1-2 Weeks)
Information on the target and host cells for the required cell line.
① Pool cells or monoclonal cells;
② Quality Inspection Report (COA) and Project Completion Report.
Specialized CRO Services
