Stable Cell Line Development Service

In contrast to transient transfection, the construction of stable cell line refers to the entry of foreign genes into recipient cells and integration into the chromosomes of the cells, allowing the target antigen to be stably expressed on the surface of the host cell membrane for a long time. Overexpression stable cell lines are prepared through lentivirus transduction and undergo standard drug screening steps, which can be used for gene function research, immune/killing/proliferation, antibody drug activity screening (cell level binding and blocking), and CAR molecule killing activity evaluation.

CUSABIO has been engaged in protein expression research for many years and has extensive experience in developing stable cell lines, which can effectively enhance the production capacity of stable cell lines, improve experimental results, and provide customers with a one-stop service from gene sequence to stable cell line delivery.

Service Advantages:

Mature Stable Cell Line Development Platform
Equipped with comprehensive service platforms for cells, antibodies, proteins, etc., it can provide one-stop service from stable cell line construction to recombinant protein production.

Strict Quality Control System
Double detection of microorganisms such as bacteria and mycoplasma, ensuring 100% contamination-free, and cell viability testing is conducted to guarantee delivery quality.

Stable Cell Passage
High quality delivery standards ensure stable passage of cells for more than 20 generations.

Professional Technical Support
Professional technical personnel regularly report on project progress to ensure smooth progress of the project.

Our Commitment:No charges if it doesn't pass the flow cytometry detection.

Service Processes:

Stable Cell Line Development Service Processes

Service content and cycle:

Step Service Content Service Details Duration
1 Customer provides host cells, and CUSABIO conducts cell testing. Cell viability, cell density, and mycoplasma testing. 1-2 weeks
2 Construction of Lentiviral Expression Plasmid Codon optimization, gene synthesis, and construction of lentiviral expression plasmid. 2-3 weeks
3 Lentivirus Packaging Co-transfer of expression plasmid and auxiliary plasmid into host cells for lentivirus packaging. 1-2 weeks
4 Cell Pool Screening and Verification Use Flag tagged antibodies or positive antibodies to screen positive cells through FACS experiments and detect cell pool expression levels. 1-2 weeks
5 Single-Clone Screening and Verification Single cell planking and verification of the expression of single clones. 2-3 weeks
6 Cell Line Quality Control Single clones are expanded, cryopreserved, and tested for post-thaw viability, bacterial, fungal, and mycoplasma contamination. 1-2 weeks
Total Time 8-14 weeks

Customer provides:

Information on the target and host cells for the required cell line.

Final deliverables:

① Pool cells or monoclonal cells;

② Quality Inspection Report (COA) and Project Completion Report.


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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
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