Stable cell lines represent a cornerstone of modern biotechnology, offering researchers a powerful and reliable platform for gene expression studies. These specialized cell lines are engineered to consistently and durably express specific genes, achieved through the integration of the target gene into the host cell genome using methods such as viral transduction or plasmid-based transfection followed by selection and maintenance of clonal cell populations. The resulting cell lines serve as invaluable laboratory tools, enabling scientists to delve into a multitude of critical applications across various scientific domains.
The versatility of stable cell lines extends far beyond the confines of the lab, finding applications in a broad spectrum of research areas. Their impact spans vital disciplines, including bio-therapeutic protein production, drug screening, and gene function research, among others. In the realm of antibody development, stable cell lines play a pivotal role as immunogens, eliciting targeted immune responses in animals, particularly for transmembrane protein targets. Moreover, these cell lines are indispensable for antibody binding and functional screening experiments, facilitating the discovery of novel treatments and therapies.