Anti-idiotype antibodies are a class of antibodies that can specifically recognize and bind to the unique epitope (idiotype) of another antibody. They have a wide range of applications in the development of therapeutic antibody drugs. Due to the similarity between anti-idiotype antibodies and anti-drug antibodies (ADAs), anti-idiotype antibodies can be used as positive controls in immunogenicity analysis for the quantification of anti-drug antibodies.
Furthermore, in pharmacokinetic (PK) and pharmacodynamic (PD) analyses of antibody drugs, anti-idiotype antibodies can be employed to detect the levels of antibody drugs in the blood (free, bound, and total forms).
Based on their binding patterns and characteristics, anti-idiotype antibodies can be categorized into three types: antigen-neutralizing anti-idiotype antibodies, antigen-non-neutralizing anti-idiotype antibodies, and drug-target complex anti-idiotype antibodies. A comparative analysis of these types is as follows:
| Type | Antigen-Neutralizing | Antigen-Non-Neutralizing | Drug-Target Complex |
| Application Range | PK Testing | Immunogenicity Evaluation | |
| Characteristics | Antibody Binding Site Specificity | Non-Antibody Binding Site Characteristics | Drug-Target Complex Specificity |
| Antigen Binding | Inhibitory and Neutralizing | Non-Inhibitory | Non-Inhibitory |
| Application | Target Blockage, Detection of Free Drug | Target Non-Blockage, Detection of Total Drug | Target Non-Blockage, Detection of Bound Drug |
| Schematic Diagram |
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| Advantages |
1. Single epitope 2. High specificity 3. Good batch-to-batch stability |
1. Can simulate real conditions in blood samples 2. Relatively short preparation period 3. Low cost |
CUSABIO has years of experience in antibody development and can customize anti-idiotype antibody development solutions for clients for pharmacokinetic (PK) and immunogenicity (ADA) analysis of antibody drugs.
In addition, based on five major protein expression platforms and a reagent kit development platform, CUSABIO can provide clients with a full range of services from antigen preparation, anti-idiotype antibody development to the establishment of detection methods and the development of reagent kits.
● Service Process
● Customer Provides
30mg full-length positive antibody
● Delivered by CUSABIO
① 1mL pre-immune serum;
② 2mg rabbit polyclonal antibodies (guaranteed titer 1:64000, purity above 95% as determined by SDS-PAGE);
③ Comprehensive test reports.
● Service Process
● Customer Provides
10mg full-length positive antibody
● Delivered by CUSABIO
① 3-5 hybridoma cell lines;
② 2mg monoclonal antibody (purity above 95% as determined by SDS-PAGE);
③ Comprehensive test reports.
● Service Process
● Customer Provides
scFv Antibody Sequences
● Delivered by CUSABIO
① 3-5 hybridoma cell lines;
② 2mg monoclonal antibody (purity above 95% as determined by SDS-PAGE);
③ Comprehensive test reports.
