Anti-idiotype Antibody Production
Technical Introduction
Anti-idiotype antibodies are a class of antibodies that can specifically recognize and bind to the unique epitope (idiotype) of another antibody. They have a wide range of applications in the development of therapeutic antibody drugs. Due to the similarity between anti-idiotype antibodies and anti-drug antibodies (ADAs), anti-idiotype antibodies can be used as positive controls in immunogenicity analysis for the quantification of anti-drug antibodies.
Furthermore, in pharmacokinetic (PK) and pharmacodynamic (PD) analyses of antibody drugs, anti-idiotype antibodies can be employed to detect the levels of antibody drugs in the blood (free, bound, and total forms).
Based on their binding patterns and characteristics, anti-idiotype antibodies can be categorized into three types: antigen-neutralizing anti-idiotype antibodies, antigen-non-neutralizing anti-idiotype antibodies, and drug-target complex anti-idiotype antibodies. A comparative analysis of these types is as follows:
| Type |
Antigen-Neutralizing |
Antigen-Non-Neutralizing |
Drug-Target Complex |
| Application Range |
PK Testing |
Immunogenicity Evaluation |
|
| Characteristics |
Antibody Binding Site Specificity |
Non-Antibody Binding Site Characteristics |
Drug-Target Complex Specificity |
| Antigen Binding |
Inhibitory and Neutralizing |
Non-Inhibitory |
Non-Inhibitory |
| Application |
Target Blockage, Detection of Free Drug |
Target Non-Blockage, Detection of Total Drug |
Target Non-Blockage, Detection of Bound Drug |
| Schematic Diagram |
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| Advantages |
1. Single epitope
2. High specificity
3. Good batch-to-batch stability
|
1. Can simulate real conditions in blood samples
2. Relatively short preparation period
3. Low cost
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CUSABIO has years of experience in antibody development and can customize anti-idiotype antibody development solutions for clients for pharmacokinetic (PK) and immunogenicity (ADA) analysis of antibody drugs.
In addition, based on five major protein expression platforms and a reagent kit development platform, CUSABIO can provide clients with a full range of services from antigen preparation, anti-idiotype antibody development to the establishment of detection methods and the development of reagent kits.
Anti-idiotype Rabbit Polyclonal Antibodies Customization
● Service Process
45-55 Business Days
Fab Preparation
2-3 Business Days
Animal Immunization
30-35 Business Days (6-7 weeks)
Potency Testing
5-7 Business Days
Antibody Purification
5-8 Business Days
Antibody Verification
2-3 Business Days
● Customer Provides
30mg full-length positive antibody
● Delivered by CUSABIO
① 1mL pre-immune serum;
② 2mg rabbit polyclonal antibodies (guaranteed titer 1:64000, purity above 95% as determined by SDS-PAGE);
③ Comprehensive test reports.
Anti-idiotype Mouse Monoclonal Antibodies Customization
——Anti-Fab Mouse Monoclonal Antibodies
● Service Process
80-100 Business Days
Fab preparation
2-3 Business Days
Animal immunization and titer testing
35-45 Business Days
Cell fusion
1 Business Days
Subcloning and cell expansion
30-40 Business Days
Isotype identification and ascites purification
15-20 Business Days
Antibody validation
2-3 Business Days
● Customer Provides
10mg full-length positive antibody
● Delivered by CUSABIO
① 3-5 hybridoma cell lines;
② 2mg monoclonal antibody (purity above 95% as determined by SDS-PAGE);
③ Comprehensive test reports.
——Anti-Scfv Mouse Monoclonal Antibodies
● Service Process
110-130 Business Days
Recombinant Expression of scFv in the Mammalian System
15-20 Business Days
Animal immunization and titer testing
35-45 Business Days
Cell fusion
1 Business Days
Subcloning and cell expansion
30-40 Business Days
Isotype identification and ascites purification
15-20 Business Days
Antibody validation
2-3 Business Days
VH/VL Sequencing
10-15 Business Days
● Customer Provides
scFv Antibody Sequences
● Delivered by CUSABIO
① 3-5 hybridoma cell lines;
② 2mg monoclonal antibody (purity above 95% as determined by SDS-PAGE);
③ Comprehensive test reports.
