Anti-idiotype Antibody Production

Technical Introduction

Anti-idiotype antibodies are a class of antibodies that can specifically recognize and bind to the unique epitope (idiotype) of another antibody. They have a wide range of applications in the development of therapeutic antibody drugs. Due to the similarity between anti-idiotype antibodies and anti-drug antibodies (ADAs), anti-idiotype antibodies can be used as positive controls in immunogenicity analysis for the quantification of anti-drug antibodies.

Furthermore, in pharmacokinetic (PK) and pharmacodynamic (PD) analyses of antibody drugs, anti-idiotype antibodies can be employed to detect the levels of antibody drugs in the blood (free, bound, and total forms).

Based on their binding patterns and characteristics, anti-idiotype antibodies can be categorized into three types: antigen-neutralizing anti-idiotype antibodies, antigen-non-neutralizing anti-idiotype antibodies, and drug-target complex anti-idiotype antibodies. A comparative analysis of these types is as follows:

Type Antigen-Neutralizing Antigen-Non-Neutralizing Drug-Target Complex
Application Range PK Testing Immunogenicity Evaluation
Characteristics Antibody Binding Site Specificity Non-Antibody Binding Site Characteristics Drug-Target Complex Specificity
Antigen Binding Inhibitory and Neutralizing Non-Inhibitory Non-Inhibitory
Application Target Blockage, Detection of Free Drug Target Non-Blockage, Detection of Total Drug Target Non-Blockage, Detection of Bound Drug
Schematic Diagram
Advantages

1. Single epitope

2. High specificity

3. Good batch-to-batch stability

1. Can simulate real conditions in blood samples

2. Relatively short preparation period

3. Low cost

CUSABIO has years of experience in antibody development and can customize anti-idiotype antibody development solutions for clients for pharmacokinetic (PK) and immunogenicity (ADA) analysis of antibody drugs.

In addition, based on five major protein expression platforms and a reagent kit development platform, CUSABIO can provide clients with a full range of services from antigen preparation, anti-idiotype antibody development to the establishment of detection methods and the development of reagent kits.

Anti-idiotype Rabbit Polyclonal Antibodies Customization

● Service Process

45-55 Business Days
Fab Preparation
Fab Preparation
2-3 Business Days
Animal Immunization
Animal Immunization
30-35 Business Days (6-7 weeks)
Potency Testing
Potency Testing
5-7 Business Days
Antibody Purification
Antibody Purification
5-8 Business Days
Antibody Verification
Antibody Verification
2-3 Business Days

● Customer Provides

30mg full-length positive antibody

● Delivered by CUSABIO

① 1mL pre-immune serum;

② 2mg rabbit polyclonal antibodies (guaranteed titer 1:64000, purity above 95% as determined by SDS-PAGE);

③ Comprehensive test reports.


Anti-idiotype Mouse Monoclonal Antibodies Customization

——Anti-Fab Mouse Monoclonal Antibodies

● Service Process

80-100 Business Days
Fab preparation
Fab preparation
2-3 Business Days
Animal immunization and titer testing
Animal immunization and titer testing
35-45 Business Days
Cell fusion
Cell fusion
1 Business Days
Subcloning and cell expansion
Subcloning and cell expansion
30-40 Business Days
Isotype identification and ascites purification
Isotype identification and ascites purification
15-20 Business Days
Antibody validation
Antibody validation
2-3 Business Days

● Customer Provides

10mg full-length positive antibody

● Delivered by CUSABIO

① 3-5 hybridoma cell lines;

② 2mg monoclonal antibody (purity above 95% as determined by SDS-PAGE);

③ Comprehensive test reports.


——Anti-Scfv Mouse Monoclonal Antibodies

● Service Process

110-130 Business Days
Recombinant Expression of scFv in the Mammalian System
Recombinant Expression of scFv in the Mammalian System
15-20 Business Days
Animal immunization and titer testing
Animal immunization and titer testing
35-45 Business Days
Cell fusion
Cell fusion
1 Business Days
Subcloning and cell expansion
Subcloning and cell expansion
30-40 Business Days
Isotype identification and ascites purification
Isotype identification and ascites purification
15-20 Business Days
Antibody validation
Antibody validation
2-3 Business Days
VH/VL Sequencing
VH/VL Sequencing
10-15 Business Days

● Customer Provides

scFv Antibody Sequences

● Delivered by CUSABIO

① 3-5 hybridoma cell lines;

② 2mg monoclonal antibody (purity above 95% as determined by SDS-PAGE);

③ Comprehensive test reports.

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7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
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