Nanodisc Technology Platform
The Nanodisc technology platform is based on artificially synthesized nanoscale lipid bilayer vesicles composed of phospholipids and membrane scaffold proteins such as Apolipoprotein A1. These bilayer vesicles stably encapsulate and immobilize membrane proteins, providing a biologically relevant environment for studying the structure and function of membrane proteins.
In buffers containing detergents, most membrane proteins can maintain stability and activity. However, a small subset of detergent-sensitive membrane proteins is not compatible with detergent-containing buffer conditions, resulting in challenges in purification, poor stability, and loss of activity. To address this, CUSABIO has specifically developed a cell-free expression-based Nanodisc technology platform. By externally adding membrane scaffold proteins (MSP), the phospholipid molecules assemble into Nanodisc structures during the reaction process, preserving the membrane proteins' native structure, functional biology, and water solubility.
Compared to traditional methods, the Nanodisc technology platform offers enhanced stability and repeatability, facilitating more accurate and reliable experimental results. The Nanodisc technology platform has broad applicability across various fields of biological research. It can be employed to investigate the structure, function, interactions, and signaling of membrane proteins. Moreover, Nanodiscs can be utilized to study membrane protein structures and drug-protein interactions during drug screening and development processes.
Read more：Nanodisc in Membrane Protein Research
- Display natural conformation with complete biological activity.
- Detergent-free, suitable for experiments that interfere with detergents.
- Can be used directly for phage screening (provided empty nanodisc reverse screening).
- Can be used for immunization/ELISA/SPR/BLI/cell assays.
CUSABIO has the following advantages in the Nanodisc technology platform:
High biological activity: The Nanodisc platform provides an environment similar to the cell membrane, showcasing the complete conformation and preserving biological activity.
High stability and compatibility: The Nanodisc platform is composed of high-quality lipids and proteins, exhibiting excellent stability and biological compatibility, and providing reliable experimental results.
Advantages in membrane protein expression: The Nanodisc technology platform offered by CUSABIO exhibits outstanding advantages in membrane protein expression. By providing an environment similar to the cell membrane, Nanodiscs can stably encapsulate and express various types of membrane proteins, including G protein-coupled receptors, ion channel proteins, and other challenging-to-express membrane proteins.