| Expression System | System Benefits | Application | Features of Cusabio |
|---|---|---|---|
| In vitro E.coli Expression System | Simple, take short time, high expression quantity, open and flexible, easy to express specific proteins, prepare protein complexes, parallel to synthesize a variety of different proteins, etc. | Toxic proteins, membrane proteins | Small amount expression conditions fumble, solve relative problems professionally, greatly reducing the experimental period, increase the expression quantity |
| E. coli Expression System | High target gene expression quantity, low cost, simple culture conditions, product rapidly, strong scalability, simple conversion operation, easy to form disulfide bond | Prokaryotic proteins, simple eukaryotic proteins | Expression includes soluble protein, inclusion body, fusion proteins, etc., with wealthy experience and expertise, we can solve a variety of bottlenecks during the protein expression process |
| Yeast Expression System | Cost-effective, low-cost for amplifying medium, simple culture conditions, production rapidly, strong scalability, good choice for secretory protein or intracellular protein expression, secrete proteins efficiently and allow simple purification, extensive post-translational modifications, no endotoxin | Industrial strain improvement, amplification | The combination of self-transformed efficient secretion vector and host can achieve the highest quality protein expression to the maximum extent; Patented Biobrick technology can be successfully used to the improvement and optimization of industrial strain |
| Insect baculovirus expression system | Large gene capacity, high efficiency of exogenous gene expression, effective cell fold, moderate scalability, extensive post-translational modifications, glycosylation similar to mammalian cells, is relatively easy enzymatic deglycosylation, no endotoxin | Virus vaccines, signal proteins, cytokines, kinases, etc. | Adopt AcNPV-sf9 cells and high5 cells two expression systems, the selectivity of multiple expression systems, multiple hosts, multi-carrier greatly improve the success rate of protein expression |
| Mammalian Cell Expression System | Higher expression levels, moderate scalability, cell suspension culture characteristic can do mass production, effective protein fold, suitable for protein secretion, full post-translational modifications, no endotoxin | Complex higher eukaryotes proteins | Adopt the specific combined methods of mammalian cell expression vector and a variety of transfection, optimize expression conditions, improve transfection efficiency, greatly shorten the experimental period, significantly increase the expression quantity |
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