What are monoclonal antibodies?
A Monoclonal antibody, by contrast, comes from a single antibody producing B cell and therefore only binds with one unique epitope. Each individual antibody in a polyclonal mixture is technically a monoclonal antibody; however, this term generally refers to a process by which the actual B-cell is isolated and fused to an immortal hybridoma cell line so that large quantities of identical antibody can be generated.
CUSABIO's monoclonal antibodies
CUSABIO specializes in antibody production and customization service. Centering on antibody, we have established a series of technology platform and animal breeding base. The monoclonal antibodies are produced by professionals who work on antibody for many years. Our mature technical route and reliable quality will save your produce time and energy. We can provide customers with high-quality monoclonal antibody products by screening and verifying every production step strictly.
Combined with our kit development platform and diagnosis reagent R & D team, we can guarantee the application of monoclonal antibody products in the field of detection. So far, we have produced a variety of monoclonal antibody materials in diagnosis reagent level, which are praised by customers deeply. Moreover, CUSABIO also offers Custom Monoclonal Antibody Production service to meet your specific requirements.
Here, we enumerate several images of validated applications for your reference.
Monoclonal antibodies vs Polyclonal antibodies
How to choose an antibody for your research? Monoclonal antibodies or polyconal antibodies, that is a problem. You need to consider their characteristics and your experiment requirements before making the decision.
The following are the advantages and disadvantages of monoclonal antibodies when they are compared with polyclonal antibodies.
- Monoclonal antibodies have a high degree of homogeneity from batch to batch, which means that if experimental conditions are kept constant, results from monoclonal antibodies can be very reproducible between experiments and batches.
- Monoclonal antibodies specifically detect a particular epitope on the antigen, and reduce probability of cross reactivity with other protein.
- Monoclonal antibodies are more suitable for experiments requiring quantification of the protein levels.
- Monoclonal antibodies production is significantly more expensive and requires more time to produce and develop the hybridized clone.
- Small changes in the epitope's structure often render the monoclonal antibody unable to detect the target protein and share across a range of species, limiting their flexibility.
- Monoclonal antibodies are more sensitive to changes in experimental conditions (ie pH and buffer composition) than polyclonal antibodies.
- Monoclonal antibodies are more vulnerable to the loss of epitope through chemical treatment of the antigen than polyclonal antibodies (this can be offset by pooling two or more monoclonal antibodies to the same antigen), - epitope changes can greatly impact binding.
- Cell culture and purification capabilities are required in the process of production.
- Monoclonal antibodies are less suitable for detecting denatured proteins than polyclonal antibodies or for use in assays that require antigen cross-linking.