Your Good Partner in Biology Research
Tel : 301-363-4651
CCR8｜C5AR1｜CD24｜CLDN18.2｜CD20｜Proteinase K｜EGFR｜PD-L1｜CD276｜Exosome Isolation Kits
Your Good Partner in Biology Research
CCR8｜C5AR1｜CD24｜CLDN18.2｜CD20｜Proteinase K｜EGFR｜PD-L1｜CD276｜Exosome Isolation Kits
Transmembrane protein (TP), also known as intact protein, is a type of membrane protein exists in the whole biofilm. Transmembrane protein plays an important role in basic physiological processes, including molecule transport, signal transduction, energy utilization, etc. On one hand, they are useful for signaling to the cell what the external environment contains. On the other hand, they serve as a way for the cell to gather information about the external environment.
Almost 30% of genes in genomic sequence encode transmembrane proteins, of which 50% are targets for currently known drugs. However, expression and purification of transmembrane protein are very difficult. And CUSABIO will help you to overcome the difficulties.
● Multiple membrane protein expression technology platforms.
Virus Like Particles (VLPs) are nanometer-scale particles formed by the automatic assembly of one or more capsid proteins of multiple viruses. VLPs do not contain viral nucleic acid, cannot replicate autonomously, have high safety, and an overall structure similar to virus particles.
Detergents are bipolar molecules, which can disintegrate lipid membranes to release membrane proteins, and provide a hydrophobic environment for membrane proteins in the state of membrane removal in solution to maintain the hydrophobic transmembrane structure of membrane proteins. This characteristic is used to solve the problem of natural conformation in TPs expression.
A few TPs that are sensitive to detergents are not suitable for buffer containing detergents, which are specifically characterized as being unable to be purified, easily degraded and inactive. For this type of membrane protein, we suggest to try the nanodiscs technology.
CUSABIO, as an experienced protein manufacturer, provides the above three technology platforms to fuel the development of transmembrane proteins. Since establishment of these platforms, 200 proteins have been successfully produced with a yield of mg/ml, which contains 99 transmembrane proteins (TPs) with 1-15 transmembrane domains and toxic proteins that are difficult to express in traditional E.coli expression systems. We have also produced high molecular weight proteins (130 kDa -140 kDa) that contain multiple transmembrane domains.
● Nearly 200 full-length membrane proteins have been successfully developed.
CLDN18.2
(4 times)
CD20
(4 times)
ACKR1
(7 times)
ADIPOR1
(7 times)
RHD
(11 times)
SLC7A11
(12 times)
● High purity determined by SDS-PAGE.
The purity of HTR1B (CSB-CF010882HUb2) (7 times) is greater than 90% as determined by SDS-PAGE with 5% enrichment gel and 15% separation gel.
-SDS.jpg)
The purity of CLCN3 (CSB-CF005482HU(A4) (15 times) is greater than 85% as determined by SDS-PAGE with 5% enrichment gel and 15% separation gel.
● High Specificity Validated by Western blot (WB).
-WB.jpg)
CLDN18.2 (CSB-MP005498HU(A5)) (4 times) is detected by Mouse anti-6*His monoclonal antibody. The three bands respectively correspond to monomer, Homodimer, Homotrimer.
CD20 (CSB-MP015007HU) (4 times) is detected by Mouse anti-6*His monoclonal antibody.
● Good bioactivity validated by Functional ELISA.
Immobilized ABCD1 (CSB-CF001068HU) (5 times) at 5 μg/ml can bind human PEX19, the EC50 of human PEX19 is 22.96-33.00 μg/ml.
Immobilized ACKR1 (CSB-CF624105HU) (7 times) at 1 μg/ml can bind human CCL2, the EC50 of human CCL2 protein is 48.64-60.24 μg/ml.
● Correct structure validated by SEM.
-TEM1.jpg)
The VLP-like structures of CLDN18.2 (CSB-MP005498HU(A5)) (4 times) has been confirmed by TEM.
The VLP-like structures of CD20 (CSB-MP015007HU) (4 times) has been confirmed by TEM.
Here is the catalog of all transmembrane proteins. You can find the right ones for your research, or search them in the search box on the header part of this website.
If you can’t find the protein you need, please check CUSABIO protein custom service.
* Browse products by selecting the initials of target names.
Transmembrane proteins are located at the interface between cells and the outside world, mediating the signal transduction between cells and the outside world, and performing many important cellular biological functions. For example, it is a receptor for various signaling molecules, hormones and other substrates; it is involved in the exchange of substances, energy and signal between the inside and outside of the cell membrane; it constitutes a channel for various ion transmembranes, which inputs nutrients and some inorganic electrolytes into cells, and discharges toxic or useless metabolites into cells.
Transmembrane protein products expressed by cell-free expression systems can be used to study the functions of transmembrane proteins. The integration of transmembrane proteins into vesicles for structural and functional studies is a hot topic in membrane protein research.
According to its structure, transmembrane proteins can be classified into alpha helix and β - barrel membrane proteins. For details, please refer to the article: A Resume for Transmembrane Proteins.
