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| Code | CSB-E12630r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 90 | ||||
| Range % | 85-100 | |||||
| 1:2000 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:4000 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8000 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-101 | ||||
| EDTA plasma (n=4) | 91 | 85-97 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit is a powerful research tool designed to detect and quantify Pulmonary surfactant-associated protein B in rat serum, plasma, and tissue homogenates. This kit is specifically designed for use in lung-related disease and signal transduction research and can detect levels of SP-B as low as 0.78 pg/mL with a detection range of 3.12 pg/mL to 200 pg/mL.
The assay principle is based on the sandwich technique, in which the capture antibody is pre-coated on a microplate and the detection antibody is labeled with biotin. Upon binding to the target analyte, the complex is captured by streptavidin-HRP conjugate and the substrate TMB is added to produce a colored reaction product that is quantified by measuring the absorbance at 450 nm.
With an assay time of 1-5 hours, a sample volume of 50-100 µl, and a detection wavelength of 450 nm, the SP-B ELISA kit provides accurate and reliable results for your research needs. With its high sensitivity and wide detection range, it can help you gain a better understanding of the role of SP-B in various physiological and pathological conditions.
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