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Product Name:

Rat glutamic acid decarboxylase(GAD) autoantibody(IgG) ELISA Kit

Product Type : ELISA Kit
Code : CSB-E08654r
Size : 96T,5×96T,10×96T
Abbreviation : GAD-Ab-IgG
Species : Rat
Sample Types : serum, plasma
Detect Range : 7.8 pg/ml-500 pg/ml
Sensitivity : 1.95 pg/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Research Area : Others
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of rat GAD autoantibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 86
Range % 81-97
1:10 Average % 89
Range % 85-100
1:20 Average % 95
Range % 90-100
1:40 Average % 93
Range % 86-99
Recovery :
The recovery of rat GAD autoantibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 82-98
EDTA plasma (n=4) 93 87-99
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 1.856 1.895 1.876 1.706
250 1.268 1.255 1.262 1.092
125 0.884 0.902 0.893 0.723
62.5 0.569 0.584 0.577 0.407
31.2 0.392 0.403 0.398 0.228
15.6 0.287 0.292 0.290 0.120
7.8 0.238 0.236 0.237 0.067
0 0.167 0.172 0.170  
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