Bovine α Lactalbumin,α-La ELISA Kit

Instructions
Code CSB-E13200B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name lactalbumin, alpha-
Alternative Names LALBA ELISA Kit; ALACTA ELISA Kit; Alpha-lactalbumin ELISA Kit; Lactose synthase B protein ELISA Kit; allergen Bos d 4 ELISA Kit
Abbreviation LALBA
Uniprot No. P00711
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 0.054 ng/ml - 40 ng/ml
Sensitivity 0.054 ng/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine α-La in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 85    
Range % 80-90    
1:2 Average % 93    
Range % 89-97    
1:4 Average % 95    
Range % 90-100    
1:8 Average % 90    
Range % 85-97    
Recovery
The recovery of bovine α-La spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 84-93    
EDTA plasma (n=4) 103 99-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
µg/ml OD1 OD2 Average      
10 0.186 0.180 0.183      
5 0.302 0.291 0.2965      
2.5 0.397 0.389 0.393      
1.25 0.523 0.495 0.509      
0.625 0.724 0.747 0.7355      
0.312 1.013 1.024 1.0185      
0.156 1.322 1.293 1.3075      
0 2.163 2.052 2.1075      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
Gene References into Functions
  1. This study shows, for the first time, that alpha-lactalbumin isolated in a rare 28kDa dimeric form induces cell death, while 14kDa monomeric alpha-lactalbumin is inactive. PMID: 28193539
  2. Macromolecular crowding reduces the calcium binding affinity of holo-lactalbumin alpha resulting in the formation of apo-lactalbumin alpha (the calcium-depleted form of holo-LA) leading to aggregate formation. PMID: 25437004
  3. Data suggest that stabilization of apo-alpha-lactalbumin by mono- and oligo-saccharides can be accounted for by simplified statistical-thermodynamic model considering volume exclusion deriving from steric repulsion between protein and saccharides. PMID: 26000826
  4. These findings suggest that the SNP alpha-LA2516 in the alpha-LA gene likely does not have potential as a molecular marker for milk production traits in Chinese Holstein cows. PMID: 24065678
  5. alphaLA forms a misfolded disulfide bond shuffled isomer, X-alphaLA which represents a different low energy fold for the protein; binding calcium plays an important role in both maintaining the native structure of alphaLA and providing a mechanism for distinguishing between folded and misfolded species. PMID: 22189830
  6. Structural features of the alphaB-crystallin oligomer when complexed with target proteins under mild stress conditions, i.e., reduction of alpha-lactalbumin at 37 degrees C and malate dehydrogenase when heated at 42 degrees C, were investigated. PMID: 21152271
  7. the organization and dynamics of the functionally important tryptophan residues of BLA in native, molten globule and denatured states utilizing the wavelength-selective fluorescence approach were explored. PMID: 20346348
  8. The complexes formed by partially folded alpha-lactalbumin with oleic acid display selective apoptotic activity against tumor cells. PMID: 19968717
  9. a functional Ca(2+)-binding site is not required for conversion of alpha-lactalbumin to the active complex or to cause cell death PMID: 14627739
  10. Negatively charged alpha-lactalbumin (LA) interaction with basic histone stabilizes apo-alpha-LA and destabilizes the Ca2+-bound protein due to compensation for excess negative charge of alpha-LA's Ca2+-binding loop by positively charged histone residues. PMID: 15134431
  11. interaction of alphaB-cyrstallin with, and promoted unfolding of, reduced alpha-lactalbumin, but showed limited chaperone activity for other target proteins PMID: 15670152
  12. Data indicate that deletion of the beta-subdomain in alpha-lactalbumin does not alter the ability of the protein to assemble into well-ordered fibrils, implying that this chain region is not essential for the amyloid formation. PMID: 15853802
  13. Fold and topology of alpha-lactalbumin may be formed from degenerate groups of side chains. PMID: 15956205
  14. residual long-range interactions may act as nucleation sites for protein folding, while this property of residual structure is replaced by the calcium-binding site between the domains in alpha-lactalbumin. PMID: 16731974
  15. characterized the acid induced molten globule state (A-state) of alpha-LA both qualitatively and quantitatively. PMID: 16741984
  16. Results describe the reaction mechanism and formation of CIDNP (chemically induced dynamic nuclear polarization) in the photoreactions of the dye 2,2'-dipyridyl with non-native states of bovine and human alpha-lactalbumins in aqueous solution. PMID: 16851644
  17. interaction between calcium-depleted alpha-lactalbumin and lysozyme leads to the formation of different supramolecular structures depending on the temperature PMID: 17260954
  18. stopped-flow CD, fluorescence, and time-resolved NMR studies that the Ca(2+)-induced refolding of bovine alpha-lactalbumin (BLA) at constant denaturant concentration (4 M urea) exhibits triple-exponential kinetics. PMID: 18377934
  19. Allelic frequencies and RFLP patterns of alpha-lactalbumin gene vary between two species of cattle. PMID: 18607792
  20. Disulfide bond shuffling in bovine alpha-lactalbumin: MD simulation confirms experiment PMID: 18942855
  21. Serotonin suppressed alpha-lactalbumin mRNA expression in lactogen-treated primary bovine mammary epithelial cell cultures. PMID: 19654143

Show More

Hide All

Subcellular Location Secreted
Protein Families Glycosyl hydrolase 22 family
Tissue Specificity Mammary gland specific. Secreted in milk.
Database Links

KEGG: bta:281894

STRING: 9913.ENSBTAP00000007701

UniGene: Bt.87412

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1