Human Apolipoprotein D(APOD) ELISA kit

Code CSB-EL001935HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P05090
Lead Time 5-7 working days
Abbreviation APOD
Protein Biological Process 1 Transport
Target Name apolipoprotein D
Species Homo sapiens (Human)
Protein Biological Process 3 Transport
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism.
HGNC 612
RGD 2137
MGI 88056
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human APOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1000Average %104
Range %100-108
1:2000Average %98
Range %94-102
1:4000Average %84
Range %80-88
1:8000Average %85
Range %81-90
The recovery of human APOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-96
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
4002.502 2.568 2.535 2.422
2002.228 2.268 2.248 2.135
1001.647 1.668 1.658 1.545
501.035 1.060 1.048 0.935
250.589 0.571 0.580 0.467
12.50.344 0.322 0.333 0.220
6.250.211 0.213 0.212 0.099
00.111 0.115 0.113
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Function APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
Subcellular Location Secreted
Protein Families Calycin superfamily, Lipocalin family
Tissue Specificity Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.
Database Links

HGNC: 612

OMIM: 107740

KEGG: hsa:347

STRING: 9606.ENSP00000345179

UniGene: Hs.522555

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