Human Leukocyte elastase inhibitor(SERPINB1) ELISA kit

Code CSB-EL021065HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
serpin peptidase inhibitor, clade B (ovalbumin), member 1
Alternative Names
Anti elastase ELISA Kit; EI ELISA Kit; ELANH2 ELISA Kit; Epididymis luminal protein 57 ELISA Kit; HEL57 ELISA Kit; ILEU_HUMAN ELISA Kit; LEI ELISA Kit; Leukocyte elastase inhibitor ELISA Kit; M/NEI ELISA Kit; MNEI ELISA Kit; Monocyte/neutrophil elastase inhibitor ELISA Kit; Peptidase inhibitor 2 ELISA Kit; Pi-2 ELISA Kit; PI2 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 ELISA Kit; Serpin B1 ELISA Kit; Serpin peptidase inhibitor clade B (ovalbumin) member 1 ELISA Kit; serpinb1 ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.625 ng/mL-40 ng/mL
0.156 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %97
Range %88-101
1:2Average %93
Range %84-97
1:4Average %91
Range %85-95
1:8Average %98
Range %90-101
The recovery of human SERPINB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10294-107
EDTA plasma (n=4)9890-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
401.768 1.700 1.734 1.606
201.409 1.434 1.422 1.294
100.886 0.944 0.915 0.787
50.565 0.551 0.558 0.430
2.50.379 0.363 0.371 0.243
1.250.245 0.252 0.249 0.121
0.6250.201 0.197 0.199 0.071
00.130 0.126 0.128
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human SERPINB1 ELISA Kit was designed for the quantitative measurement of Human SERPINB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

(From Uniprot)
Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity. During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, promotes the proliferation of beta-cells via its protease inhibitory function.
Gene References into Functions
  1. The expression of SERPINB1 was approximately 2-fold higher in apical periodontitis. SERPINB1 expression was noted in neutrophils and epithelial cells. PMID: 28673495
  2. SERPINB1 decreased inflammation, ameliorated oxidative stress in the lung, and attenuated acute lung injury in rats with orthotopic autologous liver transplantation by activating HO-1 and it does so through STAT3 PMID: 28427999
  3. a relatively low Serpinb1a protein threshold in neutrophils that is required for sustained survival PMID: 27107834
  4. SERPINB1 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  5. Data show that high serpin B1 protein (SERPINB1) gene expression was associated with favorable tumor response and prolonged survival under cisplatin-based chemotherapy. PMID: 26799424
  6. Data show that oropharyngeal squamous cell carcinomas (OPSCCs) express granzyme inhibitors SERPINB1, SERPINB4 and SERPINB9 for cytotoxicity and the expression was not different between human papillomavirus (HPV)-positive and HPV-negative tumors. PMID: 26993499
  7. Pediatric CNS-PNETs evade immune recognition by downregulating cell surface MHC-I and CD1d expression. Intriguingly, expression of SERPINB9, SERPINB1, and SERPINB4 is acquired during tumorigenesis in 29%, 29%, and 57% of the tumors PMID: 26963506
  8. is not expressed in neutrophils of both sulfur mustard-exposed and chronic obstructive pulmonary disease patients PMID: 24852194
  9. Data suggest that serine protease inhibitor (SERPIN) B1 negatively regulates glioma cell migration and invasion probably by abrogating the expression of matrix metalloproteinase-2 and the activation of focal adhesion kinase. PMID: 24968089
  10. Decreased expression of SERPINB1 correlates with tumor invasion and poor prognosis in hepatocellular carcinoma. PMID: 24105272
  11. Apoptosis-inducing factor and leukocyte elastase inhibitor derived DNase II interact and can cooperate to induce cell death. PMID: 23673989
  12. Upon reactive center loop cleavage at Phe-343,SERPINB1 covalently complexes with GzmH. SERPINB1 overexpression suppresses GzmH- or LAK cell-mediated cytotoxicity. Crystal structures show possible conformational changes in GzmH for the suicide inhibition. PMID: 23269243
  13. In the resting state during human neutrophil extracellar trap generation, SerpinB1 is exclusively in the cytoplasm, consistent with the current understanding of clade B serpins, and it may migrate and regulate events in the cell nucleus. PMID: 23002442
  14. These results suggest that serpin B1 may be a novel marker of active ulcerative colitis and may play an important role in the pathogenesis of inflammatory bowel disease. PMID: 22421620
  15. serpinB1 sustains a healthy neutrophil reserve that is required in acute immune responses. PMID: 21248149
  16. The findings define an innate role for SerpinB1 in cystic fibrosis airways. PMID: 20817705
  17. M/NEI is a dual specificity inhibitor with two adjacent reactive sites that support rapid efficient inhibitory reactions with cellular proteases, including the three neutrophil granule proteases. PMID: 11747453

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Subcellular Location
Secreted. Cytoplasm. Cytolytic granule. Early endosome.
Protein Families
Serpin family, Ov-serpin subfamily
Tissue Specificity
In human bone marrow, present in all CD45+ populations. Expression levels are highest in the neutrophil lineage, intermediate in monocytic, and lowest in lymphocytic lineage. Within the neutrophil lineage, expression is highest in promyelocytes.
Database Links

HGNC: 3311

OMIM: 130135

KEGG: hsa:1992

STRING: 9606.ENSP00000370115

UniGene: Hs.381167

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