Code | CSB-EL017647HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human PCSK9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 87 | ||||
Range % | 84-90 | |||||
1:200 | Average % | 101 | ||||
Range % | 94-106 | |||||
1:400 | Average % | 92 | ||||
Range % | 88-97 | |||||
1:800 | Average % | 100 | ||||
Range % | 95-105 |
The recovery of human PCSK9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 94-101 | ||||
EDTA plasma (n=4) | 101 | 97-103 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human PCSK9 ELISA kit employs the quantitative competitive enzyme immunoassay technique to measure the levels of human PCSK9 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity negatively correlates with the amount of PCSK9 bound in the initial step.
PCSK9 is a serine protease that acts as a ligand for the low-density lipoprotein (LDL) and is a master participant in cholesterol homeostasis. PCSK9 upregulates LDL-cholesterol levels by binding to the LDLR and promoting its lysosomal degradation. PCSK9/LDLR binding also induces lysosomal degradation inside the cells thus resulting in a reduced LDLRexpression on the cell membrane. It is an important drug target due to its crucial role in lipid metabolism. High levels of PCSK9 are linked to enhanced increased atherosclerosis progress independent of LDL levels. PCSK9 also exerts pleiotropic roles in promoting inflammation in atherosclerosis.
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