Human alpha-amylase(AMY1) ELISA Kit

Instructions
Code CSB-E14075h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name alpha-amylase
Alternative Names amylase; salivary; B ELISA Kit; 1 4 alpha D glucan glucanohydrolase ELISA Kit; 1 ELISA Kit; 1,4 alpha D glucan glucanohydrolase 2B ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; 4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; Alpha amylase ELISA Kit; Alpha-amylase 1 ELISA Kit; AMY1 ELISA Kit; AMY1_HUMAN ELISA Kit; AMY1C ELISA Kit; AMY2 ELISA Kit; AMY2B ELISA Kit; AMY3 ELISA Kit; amylase; alpha 1A (salivary) ELISA Kit; Amylase; alpha 1B (salivary) ELISA Kit; amylase; alpha 1C (salivary) ELISA Kit; amylase; salivary; alpha-1A ELISA Kit; amylase; salivary; alpha-1B ELISA Kit; amylase; salivary; alpha-1C ELISA Kit; amylase; salivary; C ELISA Kit; glycogenase ELISA Kit; HXA ELISA Kit; PA ELISA Kit; Salivary alpha-amylase ELISA Kit; salivary amylase alpha 1A ELISA Kit; salivary amylase alpha 1B ELISA Kit; salivary amylase alpha 1C ELISA Kit
Abbreviation AMY1
Uniprot No. P04745
Species Homo sapiens (Human)
Sample Types serum, saliva, tissue homogenates
Detection Range 7.8 IU/mL-500 IU/mL
Sensitivity 1.95 IU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AMY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %81-93
1:2Average %92
Range %89-96
1:4Average %90
Range %84-96
1:8Average %93
Range %86-98
Recovery
The recovery of human AMY1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10096-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
IU/mlOD1OD2AverageCorrected
5002.991 2.789 2.890 2.788
2502.607 2.604 2.606 2.504
1252.254 2.213 2.234 2.132
62.51.682 1.569 1.626 1.524
31.20.931 0.924 0.928 0.826
15.60.522 0.513 0.518 0.416
7.80.291 0.288 0.290 0.188
00.106 0.098 0.102  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Gene References into Functions
  1. 66 proteins that interact with amylase in whole saliva were identified. PMID: 29662892
  2. AMY1 copy number was significantly correlated with the variation observed in salivary amylase production and enzyme activity but did not explain the majority of observed variation between individuals. AMY1-odd and AMY1-even haplotypes showed a different relationship between copy number and expression levels, but the difference was not statistically significant. PMID: 28219410
  3. Alcohol dependent patients showed significantly lower stress-related salivary alpha-amylase activity than healthy controls. PMID: 28314952
  4. Results from 2 case-control cohorts of Chinese and Malays, show no previously reported association between AMY1 and obesity or body mass index. PMID: 27068483
  5. It was concluded that the genetic variant determining starch metabolism influences the response to weight-loss dietary intervention. Overweight and obese individuals carrying the AMY1-AMY2 rs11185098 genotype associated with higher amylase activity may have greater loss of adiposity during low-calorie diet interventions. PMID: 28659346
  6. higher levels of sAA in EHS participants PMID: 28466664
  7. Serum amylase levels in the normal range are positively associated with integrated islet beta cell function in patients with early type 2 diabetes. PMID: 27606813
  8. Our findings suggest an effect of the interaction between starch intake and AMY1 copy number on obesity. Individuals with high starch intake but low genetic capacity to digest starch had the lowest BMI, potentially because larger amounts of undigested starch are transported through the gastrointestinal tract, contributing to fewer calories extracted from ingested starch. PMID: 28539377
  9. Data show that carcinoembryonic antigen (CEA) modestly differentiated between mucinous and nonmucinous lesions, and amylase did not distinguish intraductal papillary mucinous neoplasms (IPMNs) from mucinous cystadenomas (MCAs). PMID: 26646270
  10. genetic association studies in a population of men in Republic of Korea: Data suggest that low AMY1A gene copy number is associated with high insulin resistance and thus with genetic predisposition to diabetes type 2 and metabolic syndrome. PMID: 25996848
  11. The purpose of this study was to a) determine the heart rate variability (HRV) and saliva markers of immunity (salivary immunoglobulin A; sIgA) and stress (salivary alpha-amylase; sAA) responses to chronic training in elite swimmers with a disability. PMID: 26043224
  12. Alpha-amylase in blood increases after pharmacological activation of the adrenergic pathways suggesting that sympathetic receptors are responsible for these changes. Psychological stress, however, does not seem to have an impact on alpha-amylase in blood PMID: 26110636
  13. low serum amylase level is significantly associated with increased risk of gestational diabetes mellitus PMID: 26642703
  14. Diurnal and stress-response salivary alpha amylase patterns were related to child adiposity: decreased morning alpha amylase was associated with increased BMI. PMID: 25588701
  15. Our current study suggests putative benefits of high number of AMY1 copies (and related production of salivary amylase) on energy metabolism in Mexican children. PMID: 25394825
  16. Splenic asthenia children had positive correlations between AMY1 copy number and sAA activity before or after citric acid stimulation. PMID: 26237829
  17. Results suggest that AMY1 copy number and salivary alpha-amylase (sAA) amount played crucial roles in sAA activity; however, the roles were attenuated after stimulation due to fortified release of glycosylated sAA PMID: 25446200
  18. Elevation in AMY1a following a second bout of downhill running may indicate an adaptive stress response which reduces the inflammatory events resulting from this type of exercise or an exercise-induced enhancement in innate secretory immunity. PMID: 25145588
  19. findings suggest that both affective arousal andvalence should be accounted for when examining differences in salivary alpha Amylase reactivity and diurnal patterns. PMID: 25076484
  20. genetic association study in population in China: Data suggest that copy number variation of AMY1A gene is not associated with down-regulation of salivary response in thin children as compared to salivary response in normal weight children. PMID: 25784372
  21. Salivary alpha amylase increased during wound care procedures of pediatric burn patients, and correlated with self-report of pain. PMID: 24433940
  22. results show a strong association between acute physical stressor-induced elevations in sAA levels and patients with MDD. PMID: 22063648
  23. These findings confirm that sAA is sensitive to the effects of potentially stressful stimuli (state variance) and at the same time demonstrate its relative robustness and stability across time and conditions (trait variance). PMID: 21827821
  24. Low copy number of the salivary amylase gene predisposes to obesity. PMID: 24686848
  25. AMY1A can be of great diagnostic utility when trying to differentiate Chromophobe renal cell carcinoma and oncocytoma PMID: 24225843
  26. Excercise significantly elevated salivary alpha-amylase in patients with mild cognitive impairment and normal aging controls. PMID: 22914593
  27. A review of the possible impact of copy number polymorphism of the AMY1 gene on nutritional issues such as starch digestion, effect on glycemic response after starch consumption, effect on taste perception and satiety, influence on psychosocial stress and relation to oral health. PMID: 22965187
  28. Adults who indicated that they would respond in a harsh way to the crying infant were significantly less likely to show a decrease in salivary alpha amylase. PMID: 23144191
  29. Salivary alpha-amylkase levels in the responder group were significantly elevated compared with the non-responder group and controls both before and after electrical stimulation in panic disorder patients. PMID: 22391145
  30. High amylase individuals may be better adapted to ingest starches, whereas low amylase individuals may be at greater risk for insulin resistance and diabetes if chronically ingesting starch-rich diets. PMID: 22492122
  31. Regression analyses demonstrated significant curvilinear relations and interactions between cortisol and sAA in the prediction of child functioning. PMID: 22100627
  32. salivary alpha-amylase increases may reflect levels of emotional arousal, including high arousal positive emotions PMID: 21840370
  33. show that AMY1 copy number relates to salivary amylase concentration and enzymatic activity level, which, in turn, account for individual variation in the oral perception of starch viscosity PMID: 20967220
  34. the binding region of human salivary alpha-amylase is composed of four glycone and three aglycone-binding sites, while that of mutant Tyr151Met is composed of four glycone and two aglycone-binding sites PMID: 12782315
  35. This study reports on the effectiveness and specificity of glucopyranosylidene-spiro-thiohydantoin (G-TH) inhibitor on the 2-chloro-4-nitrophenyl-4-O-beta-D-galactopyranosyl-maltoside (GalG(2)CNP) hydrolysis catalysed by human salivary alpha-amylase. PMID: 14637141
  36. These results suggest that the residue Trp58 plays a critical role in substrate binding and hydrolytic activity of human salivary alpha-amylase. PMID: 15182367
  37. Data show that tannin is an effective inhibitor of human salivary alpha-amylase and indicate a higher stability for the enzyme-inhibitor complex than the enzyme-substrate-inhibitor complex. PMID: 15194503
  38. A marked increasse in salivary alpha-amylase activity due to psychosocial stress and a reaction significantly different from a nonstress condition is observed. PMID: 15708646
  39. The addition of salivary alpha-amylase to culture supernatants of S. gordonii precipitated a protein complex containing amylase, AbpA, amylase-binding protein B (AbpB), and the glucosyltransferase produced by S. gordonii (Gtf-G). PMID: 17593303
  40. copy number of the salivary amylase gene (AMY1) is correlated positively with salivary amylase protein level and that individuals from populations with high-starch diets have, on average, more AMY1 copies than those with traditionally low-starch diets PMID: 17828263
  41. Social evaluation-responders allocated significantly more money than controls; while there was no significant correlation between social evaluation-induced salivary amylase elevation and the allocated money. PMID: 17984929
  42. The crystal structure of HSAmy-ar (multiple mutant) as an acarbose complex was determined at a resolution of 1.5 A and compared with the existing wild-type acarbose complex. PMID: 18951906

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Subcellular Location Secreted
Protein Families Glycosyl hydrolase 13 family
Database Links

HGNC: 474

OMIM: 104700

KEGG: hsa:276

STRING: 9606.ENSP00000359100

UniGene: Hs.599274

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