Human alpha-amylase(AMY1) ELISA Kit

Human alpha-amylase(AMY1) ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Human alpha-amylase(AMY1) ELISA Kit

Name: Human alpha-amylase(AMY1) ELISA Kit
Brand: CUSABIO
SKU: CSB-E14075h

Citations (4) Protocol MSDS

Code	CSB-E14075h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (4)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

alpha-amylase

Alternative Names

amylase; salivary; B ELISA Kit; 1 4 alpha D glucan glucanohydrolase ELISA Kit; 1 ELISA Kit; 1,4 alpha D glucan glucanohydrolase 2B ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; 4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; Alpha amylase ELISA Kit; Alpha-amylase 1 ELISA Kit; AMY1 ELISA Kit; AMY1_HUMAN ELISA Kit; AMY1C ELISA Kit; AMY2 ELISA Kit; AMY2B ELISA Kit; AMY3 ELISA Kit; amylase; alpha 1A (salivary) ELISA Kit; Amylase; alpha 1B (salivary) ELISA Kit; amylase; alpha 1C (salivary) ELISA Kit; amylase; salivary; alpha-1A ELISA Kit; amylase; salivary; alpha-1B ELISA Kit; amylase; salivary; alpha-1C ELISA Kit; amylase; salivary; C ELISA Kit; glycogenase ELISA Kit; HXA ELISA Kit; PA ELISA Kit; Salivary alpha-amylase ELISA Kit; salivary amylase alpha 1A ELISA Kit; salivary amylase alpha 1B ELISA Kit; salivary amylase alpha 1C ELISA Kit

Abbreviation

AMY1

Uniprot No.

P04745

Species

Homo sapiens (Human)

Sample Types

serum, saliva, tissue homogenates

Detection Range

7.8 IU/mL-500 IU/mL

Sensitivity

1.95 IU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AMY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-93
1:2	Average %	92
	Range %	89-96
1:4	Average %	90
	Range %	84-96
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

IU/ml	OD1	OD2	Average	Corrected
500	2.991	2.789	2.890	2.788
250	2.607	2.604	2.606	2.504
125	2.254	2.213	2.234	2.132
62.5	1.682	1.569	1.626	1.524
31.2	0.931	0.924	0.928	0.826
15.6	0.522	0.513	0.518	0.416
7.8	0.291	0.288	0.290	0.188
0	0.106	0.098	0.102

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human AMY1 ELISA Kit was designed for the quantitative measurement of Human AMY1 protein in serum, saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 IU/mL-500 IU/mL and the sensitivity is 1.95 IU/mL.

Entry Into Boarding Preschool Is Associated With Increased Stress and School Refusal N Xiao, X Zhang, CLW Chan,Stress and Health,2025
Utility of Veillonella parvula polymerase chain reaction in diagnosing aspiration pneumonia: a prospective observational study YK Wu, MC Yang, HW Chung, CC Lan,ERJ Open Research,2025
Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada M Abdel-Tawwab,Scientific reports,2023
Effect of salivary antimicrobial factors on microbial composition of tongue coating in patients with coronary heart disease with phlegm-stasis syndrome J Ye,World Federation of Chinese Medicine Societies,2023

■ Customer Reviews

Average Rating:

5.0 - 4 reviews

Submit a Review here

Sample type: Tissue homogenate(saliva)

Sample species: Human

Sample dilution: 1:50

Review: I used cSB-E14075h to detect human salivary amylase index. The OD values of the samples were 0.065, 0.102, 0.209, 0.324, 0.524, 0.924, 1.578, 2.14.

By Anonymous

Sample type: Tissue homogenate(saliva)

Sample species: Human

Sample dilution: No dilution

Review: I used cSB-E14075h to detect the concentration of salivary amylase in infertile people. The OD values of the standard substance were 0.082, 0.191, 0.348, 0.553, 0.966, 1.645, 2.134, 2.838, and the sample concentrations were all within the normal range

By Anonymous

Sample type: Tissue homogenate(saliva)

Sample species: Human

Sample dilution: No dilution

Review: I used cSB-E14075h to detect salivary amylase in infertile people. The OD value of standard substance was 0.085, 0.22, 0.321, 0.409, 0.637, 1.053, 1.53, 2.129. Several of the sample OD values were outside the upper limit

By Anonymous

Sample type: Tissue homogenate(saliva)

Sample species: Human

Sample dilution: No dilution

Review: cSB-E14075h was used to detect the concentration of salivary amylase in infertile people. The OD values of standard samples were 0.07, 0.23, 0.316, 0.605, 0.907, 1.325, 1.914, 2.51, and the OD values of some samples exceeded the upper limit

By Anonymous

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.