Human alpha-amylase(AMY1) ELISA Kit

Code CSB-E14075h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
alpha-amylase
Alternative Names
amylase; salivary; B ELISA Kit; 1 4 alpha D glucan glucanohydrolase ELISA Kit; 1 ELISA Kit; 1,4 alpha D glucan glucanohydrolase 2B ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; 4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; Alpha amylase ELISA Kit; Alpha-amylase 1 ELISA Kit; AMY1 ELISA Kit; AMY1_HUMAN ELISA Kit; AMY1C ELISA Kit; AMY2 ELISA Kit; AMY2B ELISA Kit; AMY3 ELISA Kit; amylase; alpha 1A (salivary) ELISA Kit; Amylase; alpha 1B (salivary) ELISA Kit; amylase; alpha 1C (salivary) ELISA Kit; amylase; salivary; alpha-1A ELISA Kit; amylase; salivary; alpha-1B ELISA Kit; amylase; salivary; alpha-1C ELISA Kit; amylase; salivary; C ELISA Kit; glycogenase ELISA Kit; HXA ELISA Kit; PA ELISA Kit; Salivary alpha-amylase ELISA Kit; salivary amylase alpha 1A ELISA Kit; salivary amylase alpha 1B ELISA Kit; salivary amylase alpha 1C ELISA Kit
Abbreviation
AMY1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, saliva, tissue homogenates
Detection Range
7.8 IU/mL-500 IU/mL
Sensitivity
1.95 IU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AMY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %81-93
1:2Average %92
Range %89-96
1:4Average %90
Range %84-96
1:8Average %93
Range %86-98
Recovery
The recovery of human AMY1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10096-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
IU/mlOD1OD2AverageCorrected
5002.991 2.789 2.890 2.788
2502.607 2.604 2.606 2.504
1252.254 2.213 2.234 2.132
62.51.682 1.569 1.626 1.524
31.20.931 0.924 0.928 0.826
15.60.522 0.513 0.518 0.416
7.80.291 0.288 0.290 0.188
00.106 0.098 0.102  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human AMY1 ELISA Kit was designed for the quantitative measurement of Human AMY1 protein in serum, saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 IU/mL-500 IU/mL and the sensitivity is 1.95 IU/mL.

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