Human hepatitis D virus(HDV) antibody(IgG) ELISA Kit

Instructions
Code CSB-E04809h
Size
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The Human hepatitis D virus (HDV) IgG ELISA kit is used for qualitative identification of IgG antibodies to HDV in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HDV antigen. Samples or standards are pipetted into the wells with anti-human IgG conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human HDV IgG antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. The valence of human HDV IgG antibody in the samples is determined by referring to the negative control. It indicates the presence of HDV IgG antibody if the O.D. (optical density) of sample is greater than or equal to the cutoff value (average Negative Control value plus 0.10). There is no HDV IgG antibody present in the sample if the O.D. is less than the cutoff value.

This assay has high sensitivity and excellent specificity for detection of human HDV IgG antibody. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.

HDV is a defective RNA virus and is comprised of a delta antigen (viral core) and a hepatitis B surface antigen (HBsAg) (viral protein coat). HBV is required to initiate and maintain the replication of HDV in the infected liver cells. HDV is also known as a "satellite virus" because HDV-infected patients are usually infected with HBV. The diagnosis of HDV can be done by detecting the HDV antigen, HDV-specific IgM, or HDV-specific total antibodies (IgM and IgG) in the serum of patients with clinically obvious acute or chronic hepatitis B infection. Anti-HDV IgM usually appears in serum at 2 to 3 weeks after onset of symptoms and disappears by 2 months after acute HDV infection. IgM antibodies may persist for 9 months in HDV reinfection. HDV IgG and HDV total antibodies last in serum after the resolution of acute HDV infection and in chronic coinfection. IgG antibodies usually appear in the recovery period after a few weeks.

Target Name hepatitis D virus(HDV) antibody(IgG)
Alternative Names N/A
Abbreviation HDV Ab (IgG)
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range Request Information
Sensitivity Request Information
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Microbiology
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Typical Data
             
             
Test parameter specification test result  
Positive control >0.6 0.842  
Negative control <0.1 0.051  
Positive rate 10,Positive 100%  
Negative rate 10,Negative 100%  
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with HDV antigen.
  • Negative Control (1 x 1ml) --Eliminate false positive
  • Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
  • HRP-conjugated IgG antibody(1 x 12ml) --Bind to the anti-HDV IgG antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
  • Sample Diluent (1x 12 ml) --Dilute the sample to an appropriate concentration.
  • Wash Buffer (20x concentrate) (1 x 50 ml) --Wash away unbound or free substances.
  • Substrate A (1 x 6 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
  • Substrate B (1 x 6 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
  • Stop Solution (1 x 6ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 630 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1