Monkey Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit

Monkey Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit

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Monkey Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit

Protocol

Code	CSB-EL017647RH
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Alternative Names

PCSK9 ELISA Kit; Proprotein convertase subtilisin/kexin type 9 ELISA Kit; EC 3.4.21.- ELISA Kit; Proprotein convertase 9 ELISA Kit; PC9 ELISA Kit; Subtilisin/kexin-like protease PC9 ELISA Kit

Abbreviation

PCSK9

Uniprot No.

A8T666

Species

Monkey

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.47 ng/mL-15 ng/mL

Sensitivity

0.235 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of monkey PCSK9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:20	Average %	95
	Range %	89-103
1:40	Average %	92
	Range %	88-105
1:80	Average %	97
	Range %	92-108
1:160	Average %	101
	Range %	96-110

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
15	3.212	3.171	3.191	3.088
7.5	2.220	2.249	2.235	2.131
3.75	1.341	1.402	1.371	1.267
1.88	0.885	0.878	0.881	0.777
0.94	0.457	0.449	0.453	0.349
0.47	0.264	0.275	0.270	0.166
0	0.107	0.101	0.104

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Monkey PCSK9 ELISA Kit was designed for the quantitative measurement of Monkey PCSK9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.1 pg/mL-800 pg/mL and the sensitivity is 1.1 pg/mL.

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Function
(From Uniprot)

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.

Subcellular Location

Cytoplasm. Secreted. Endosome. Lysosome. Cell surface. Endoplasmic reticulum. Golgi apparatus.

Protein Families

Peptidase S8 family

Database Links

KEGG: mcc:717147

UniGene: Mmu.17333

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

Cholesterol metabolism

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.