Mouse Agouti-related protein(AGRP) ELISA kit

Instructions
Code CSB-EL001462MO
Size 96T,5×96T,10×96T
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Product Details

Target Name agouti related protein homolog (mouse)
Alternative Names Agrp ELISA kit; Agrt ELISA kit; ArtAgouti-related protein ELISA kit
Abbreviation AGRP
Uniprot No. P56473
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-101
1:2Average %95
Range %89-100
1:4Average %93
Range %87-99
1:8Average %94
Range %88-99
Recovery
The recovery of mouse AGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)9285-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.594 2.568 2.581 2.404
5001.925 1.899 1.912 1.735
2501.213 1.178 1.196 1.019
1250.807 0.789 0.798 0.621
62.50.454 0.424 0.439 0.262
31.20.317 0.302 0.310 0.133
15.60.264 0.254 0.259 0.082
00.178 0.176 0.177  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity (By similarity). It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner (By similarity).
Gene References into Functions
  1. nalysis of hypothalamic and neuroendocrine responses to HFS throughout the light-dark cycle suggests uncoupling of hypothalamic responses involving appetite-stimulating fasting-responsive hypothalamic neurons expressing agouti-related peptide (AgRP) and neuropeptide Y (Npy). PMID: 28294152
  2. By using mutant mice with liver-specific ablation of Eralpha, the authors here demonstrate that the hepatic ERalpha is essential for the modulation of the activity of Agouti Related Protein (AgRP) neurons in relation to the reproductive cycle and diet. PMID: 28446774
  3. The results demonstrate a novel role of PDK1 in AgRP neurons to counteract the high salt diet-induced hypertension by preventing hyperactivation of paraventricular nucleus nesfatin-1 neurons. PMID: 29705701
  4. The findings are consistent with the idea that, during metabolic deficiency, AgRP signaling contributes to infertility by inhibiting Kiss1 neurons. PMID: 28196880
  5. POMC and AgRP neurons receive direct steroid- and frequency-dependent glutamatergic synaptic input from Kiss1(ARC) neurons in male mice PMID: 27093227
  6. P2Y6 signaling in AgRP neurons is involved in the onset of obesity-associated hyperphagia. PMID: 28199831
  7. These results identify SK3 as a key intrinsic mediator that coordinates nutritional status with AgRP/NPY neural activities and animals' feeding behavior and energy metabolism. PMID: 27829152
  8. It was concluded that a small population of neuroendocrine cells in the adrenal medulla, and the arcuate nucleus of the hypothalamus, express AgRP and neuropeptide Y and are functionally involved in the systemic response to fasting. PMID: 28531318
  9. define a disynaptic network originating from a subset of Agouti-related peptide neurons in the hypothalamus that project to the medial nucleus of the amygdala and then to the principal bed nucleus of the stria terminalis, which suppresses territorial aggression and reduces contextual fear. PMID: 27019015
  10. These findings indicate that the relevance of AgRP to POMC neuron GABA connectivity depends on the state of AgRP neuron activity and suggest that different types of transmitter release should be considered when circuit mapping. PMID: 28667175
  11. CRFR1, in a subset of AgRP neurons, plays a regulatory role that enables appropriate sympathetic nervous system activation and consequently protects the organism from hypothermia and hypoglycemia. PMID: 27211900
  12. Diet composition, not calorie intake, rapidly alters intrinsic excitability of hypothalamic AgRP/NPY neurons in mice. PMID: 26592769
  13. Data show that agouti-related protein (AgRP)-glucocorticoid receptor knockout (GR-/-) mice are resistant to diet-induced obesity. PMID: 26889940
  14. Data show that chronic caloric restriction (CR) alters hypothalamic agouti-related protein (Agrp) and neuropeptide Y (Npy) gene expression similarly in Ghrelin+/+, Ghrelin-/-, ghrelin receptor Ghsr+/+, and Ghsr-/- mice. PMID: 26812158
  15. Suppression of sympathetic tone in AgRP-Sirt1/(--) mice reversed osteopenia in transgenic animals. PMID: 26411686
  16. Data (including data from studies in transgenic/knockout mice) suggest that expression in adult brain neurons (especially in parabrachial nucleus) of both Dmbx1 (deleted in malignant brain tumors 1) and AgRP is important in appetite regulation. PMID: 26505115
  17. mTORC1 in Agrp neurons controls circadian expression of orexigenic neuropeptides but is dispensable for the regulation of feeding behavior and energy metabolism PMID: 26159924
  18. Absence of Mc3rs (but not Ghsrs) was associated with lower AgRP/Npy expression, suggesting attenuated responses to signals of negative energy balance. PMID: 25211592
  19. These experiments reveal both overlapping and nonredundant effects of JNK- and IKK-dependent signaling in AgRP neurons. PMID: 25456138
  20. Agrp is increased in arcuate hypothalamus during food deprivation. PMID: 25788674
  21. The enhanced leptin levels during pregnancy in mice are associated with retardation of obesity development in obesity prone male offspring and with changes in fetal weight and AgRP gene expression in placenta. PMID: 25464737
  22. Inhibitors of NOS and the cystic fibrosis transmembrane conductance regulator (CFTR) prevented a decrease in AgRP secretion with glucose, suggesting a pivotal role for nNOS and the CFTR in glucose-sensing. PMID: 24145125
  23. Chemogenetic stimulation of paraventricular nucleus neurons expressing TRH and PACAP in sated mice, markedly activates AgRP neurons and induces intense feeding PMID: 24487620
  24. AGRP imposes an inhibitory effect on puberty.Agrp(-/-) Lepr(db/db) females are fertile and sustain adequate nutrition of pups with lactation to weaning age. PMID: 24169048
  25. In the hypothalamus, decreases in mTORC1 activity increase agouti-related protein mRNA expression. PMID: 22456226
  26. ATF3 plays an important role in the control of glucose and energy metabolism by regulating Agrp PMID: 23462798
  27. Data indicate that ablation of agouti-related protein (AgRP) neurons results in enhanced expression of NR2B along with a modest suppression of NR2A. PMID: 23964123
  28. Data from transgenic/knockout mice suggest AgRP (agouti-related peptide) in hypothalamic neurons is involved (via ROCK1 [rho-associated protein kinase 1] activation) in homeostatic regulation of energy expenditure and adiposity/obesity. PMID: 23885017
  29. Agouti related peptide is a common orexigenic target gene of glucocorticoid receptor and Bsx in the mouse hypothalamus. PMID: 23671185
  30. Study finds that transcription factor FoxO1 exerts integrative control of leptin, insulin, and amino acid signaling in AgRP neurons, effectively coordinating food intake with peripheral metabolism; and identifies of a heretofore unrecognized FoxO1 pathway that regulates food intake through the G protein-coupled receptor, Gpr17. PMID: 22682251
  31. comparison of chicken, human, and mouse Agrp PMID: 10771094
  32. heparanase acts as a negative modulator of AgRP signaling at MC4R, through cleavage of heparan sulfate chains presumably linked to syndecan-3 PMID: 22479599
  33. A previously unsuspected role is revealed for hypothalamic hunger-promoting Agrp neurons in setting midbrain dopamine neuronal activity and nonfood-related behaviors. PMID: 22729177
  34. AgRP-expressing neurons play a critical role in mediating the metabolic syndrome and infertility of Lep(ob/ob) mice, whereas MCH-expressing neurons have only a minor role PMID: 22232663
  35. we demonstrate a role for autophagy in hypothalamic agouti-related peptide neurons in the regulation of food intake and energy balance. PMID: 21803288
  36. PDK1 and Foxo1 signaling pathways play important roles in the control of energy homeostasis through AGRP-independent mechanisms PMID: 21694754
  37. Data show that the phenotype of a mouse lacking both POMC and AgRP (DKO) is indistinguishable from that of mice lacking Pomc alone. PMID: 21363936
  38. activating AgRP neurons potently increased motivation for feeding and also drove intense food-seeking behavior, demonstrating that AgRP neurons engage brain sites controlling multiple levels of feeding behavior PMID: 21364278
  39. This study evaluated whether neuropeptide Y or agouti-related protein affect hyperphagia and energy metabolism in leptin receptor-deficient mice. PMID: 21285324
  40. Data indicate that AGRP neurons directly engage feeding circuits and serve a dedicated role coordinating this complex behavior. PMID: 21209617
  41. Kruppel-like factor 4 augments hypothalamic AGRP and stimulates appetite in mice. PMID: 20980461
  42. These data demonstrate that the increased preference for a high fat diet after stimulation of MOR is attenuated in the absence of AgRP, but the increase in food intake (i.e. hyperphagia) is not PMID: 20051234
  43. effects of changing type and amount of dietary fats on AgRP mRNA expression PMID: 12006366
  44. Neither AgRP nor NPY is a critically required orexigenic factor, suggesting that other pathways capable of regulating energy homeostasis can compensate for the loss of both AgRP and NPY. PMID: 12077332
  45. Agrp mRNA expression was decreased relative to wild-type controls in neonates of both sexes of Prader-Willi syndrome mice PMID: 12443978
  46. Data support the hypothesis that syndecan-3 modulates melanocortin activity via facilitation of agouti-related protein. PMID: 12851299
  47. Although deletion of either NPY or AgRP caused only a modest or nondetectable effect, ablation of both ligands completely abolished the orexigenic action of ghrelin. PMID: 14962995
  48. identification of a novel feedback loop and reciprocal transcriptional regulation between agouti-related protein and leptin centrally and peripherally PMID: 15178437
  49. Data show that agouti-related protein mRNA levels are significantly lower in obese tub/tub mice as compared to tub/+ mice. PMID: 15193430
  50. AGRP signaling to thyroid releasing hormone hypophysiotropic neurons in the paraventricular nucleus is primarily mediated by the MC4-R PMID: 15256492
  51. a 42.5-kb region upstream of Agrp, containing three distinct regions that are evolutionarily conserved between mouse and human PMID: 15345681
  52. Ectopic Agouti protein overexpression increased stimulated corticosterone production and intracellular steroidogenic enzyme reactivity to cAMP without an effect on adenylate cyclase activity. PMID: 15538940
  53. leptin-dependent regulation of the intrinsic action potential frequency of orexigenic arcuate nucleus neuropeptide Y/Agouti-related protein neurons is induced by fasting PMID: 15591135
  54. Levels of expression altered in fetuses from pregnant dams on a protein-restricted diet. PMID: 16099070
  55. postembryonic partial loss of AgRP/NPY neurons leads to a lean, hypophagic phenotype PMID: 16099943
  56. differential uptake of AgRP by peripheral organs could be a regulated process that is modulated by food deprivation PMID: 16141394
  57. Agouti-related protein has a role in body composition PMID: 16330327
  58. Data show that delivery of a constitutively active mutant FoxO1 to the arcuate nucleus results in a loss of the ability of leptin to curtail food intake and suppress expression of agouti-related protein. PMID: 16604086
  59. Signal transducer and activator of transcription (stat) binding sites but not stat3 are required for fasting-induced transcription of agouti-related protein messenger ribonucleic acid. PMID: 16709597
  60. It is concluded that although AgRP(-/-) mice become morbidly obese consuming a high fat diet, they seem to overcome obesity- and age-related pathologies and live significantly longer than their metabolically similar wild type littermates. PMID: 17097059
  61. Dmbx1 is essential for various actions of agouti-related protein and plays a role in normal regulation of energy homeostasis and behavior PMID: 17873059
  62. ablation of AgRP neurons after lactation commences results in rapid starvation, indicating that both basal feeding and lactation-induced hyperphagia become dependent on AgRP neurons in adulthood PMID: 17974621
  63. cytoplasmic or nuclear NADH, uniquely produced by glucose metabolism, mediates effects of glucose on AgRP expression PMID: 17974626
  64. the aberrant appearance of the AgRP system in the anorexia mouse in the early postnatal development could involve a microglia-associated process and the innate immune system PMID: 18098136
  65. AgRP/NPY and POMC neurons each play mandatory roles in aspects of leptin-regulated energy homeostasis. PMID: 18162515
  66. both the appetitive and consummatory aspects of feeding become impaired in a melanocortin-independent manner after AgRP neuron ablation PMID: 18272480
  67. Demonstrate that Stat3-dependent signaling in AgRP neurons in the arcuate nucleus controls locomotor activity independently of AgRP regulation. PMID: 18316029
  68. AgRP neurons may participate with arcuate nucleus proopiomelanocortin neurons in mediating the anorexic and metabolic responses to acute and chronic disease processes PMID: 18583425
  69. GABA release from AgRP neurons is important in regulating energy balance. PMID: 19160495
  70. The mechanisms involved in the regulation of the NPY/AgRP neuron by insulin, were studied. PMID: 19524132
  71. endogenous AgRP signaling modulates the reinforcing properties of ethanol and binge-like ethanol drinking PMID: 19566712
  72. although ERalpha is the mediator of estrogen's anorexigenic effects, expression of ERalpha is completely excluded from AgRP and NPY hypothalamic neurons, suggesting that estrogen may regulate indirectly via presynaptic neurons that express ERalpha PMID: 19805233

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Subcellular Location Secreted, Golgi apparatus lumen
Tissue Specificity Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.
Database Links

KEGG: mmu:11604

STRING: 10090.ENSMUSP00000005849

UniGene: Mm.441696

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